SMC, airway (HASM), human

Export

Primary smooth muscle cells isolated from human airway

Cell Type:
Smooth muscle cell
Tissue Origin:
lung
Species:
human
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic A-033 7e5 63% 52% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (10)

A search for novel cancer/testis antigens in lung cancer identifies VCX/Y genes, expanding the repertoire of potential immunotherapeutic targets
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Taguchi A1, Taylor AD2, Rodriguez J3, Celiktas M4, Liu H, Ma X, Zhang Q, Wong CH, Chin A, Girard L, Behrens C, Lam WL, Lam S , Minna JD, Wistuba II, Gazdar AF, Hanash SM. 
In:
Cancer Res (2014) 74(17): 4694-705 
High throughput RNAi assay optimization using adherent cell cytometry
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nabzdyk CS, Chun M, Pradhan L, Logerfo FW 
In:
J Transl Med (2011) 9(48): 1-9 
Airway epithelium stimulates smooth muscle proliferation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Malavia NK, Raub CB, Mahon SB, Brenner M, Panettieri RA Jr, George SC. 
In:
Am J Respir Cell Mol Biol (2009) 41(3): 297-304 
Cytokines induce an early steroid resistance in airway smooth muscle cells: novel role of interferon regulatory factor-1
Categories:
Transfection 
Authors:
Tliba O, Damera G, Banerjee A, Gu S, Baidouri H, Keslacy S, Amrani Y 
In:
Am J Respir Cell Mol Biol (2008) 38(4): 463-72 
Nox4 mediates TGF-beta1-induced retinoblastoma protein phosphorylation, proliferation, and hypertrophy in human airway smooth muscle cells
Categories:
Transfection 
Authors:
Sturrock A, Huecksteadt TP, Norman K, Sanders KA, Murphy TM, Chitano P, Wilson K, Hoidal JR, Kennedy TP 
In:
Am J Physiol Lung Cell Mol Physiol (2007) 292(6): L1543-55 
Corticosteroid Inhibition of Growth-Related Oncogene Protein- via Mitogen-Activated Kinase Phosphatase-1 in Airway Smooth Muscle Cells
Categories:
Transfection 
Authors:
Issa R, Xie S, Khorasani N, Sukkar M, Adcock IM, Lee KY, Chung KF 
In:
J Immunol (2007) 178(11): 7366-75 
Inhibition of Tumor Necrosis Factor-alpha-Inducible Inflammatory Genes by Interferon-gamma Is Associated with Altered Nuclear Factor-B Transactivation and Enhanced Histone Deacetylase Activity
Categories:
Transfection 
Authors:
Keslacy S, Tliba O, Baidouri H, Amrani Y 
In:
Mol Pharmacol (2007) 71(2): 609-618 
Sub-cellular distribution of the TSC-2 gene product tuberin is driven by multiple localisation sequences and is cell cycle dependent
Categories:
Transfection 
Authors:
Clements D, Mayer RJ, Johnson S 
In:
Am J Physiol Lung Cell Mol Physiol (2007) 292(1): L258-66 
Transcriptional regulation of CD38 expression by tumor necrosis factor- in human airway smooth muscle cells: role of NF-B and sensitivity to glucocorticoids
Categories:
Transfection 
Authors:
Kang BN, Tirumurugaan KG, Deshpande DA, Amrani Y, Panettieri RA, Walseth TF, Kannan MS 
In:
FASEB J (2006) 20(7): 1000-2 
CD38 Expression Is Insensitive to Steroid Action in Cells Treated with Tumor Necrosis Factor- and Interferon- by a Mechanism Involving the Up-Regulation of the Glucocorticoid Receptor beta Isoform
Categories:
Transfection 
Authors:
Tliba O, Cidlowski J and Amrani Y 
In:
Mol Pharmacol (2006) 69(2): 588-596 
+ Show All

Privacy | Legal | About Lonza

© Lonza. All rights reserved.