WEHI-231
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B lymphoma cell line, immature, can form multicell aggregates
Cell Type:
B cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
L
C-005
2e6
66-88%
57-67%
Plasmid (general)
2 µg
100 µl
I/II/2b
SG
CP-100
4e5
76-82%
66-78%
Plasmid (general)
0.8 µg
20 µl
4D X-Unit
SG
CP-100
2e6
82-86%
66-74%
Plasmid (general)
4 µg
100 µl
4D X-Unit
Citations
The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis
Categories:
Transfection
Authors:
Avramidou A, Kroczek C, Lang C, Schuh W, Jäck HM, Mielenz D
In:
Cell Death Differ (2007) 14(11): 1936-47
Mechanosensitive activation of K+ channel via phospholipase C-induced depletion of phosphatidylinositol 4,5-bisphosphate in B lymphocytes
Categories:
Transfection
Authors:
Nam JH, Lee HS, Nguyen YH, Kang TM, Lee SW, Kim SJ, Kim HY, Earm YE, Kim SJ
In:
J Physiol (2007) 582(Pt 3): 977-90