T-47D

Mammary gland ductal carcinoma. Isolated by I. Keydar from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast.

Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V X-005 1e6 48-54% 93-95% Plasmid (general) 2 µg 100 µl I/II/2b
SE 96-FF-150 2e5 80% Plasmid (general) 20 µl Shuttle
SE EN-104 2e5 72-74% 64-74% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE EN-104 1e6 81-87% 56-76% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Gu W, Wells AL, Pan F, Singer RH 
In:
Mol Cell Biol (2008) 28(16): 4963-74 
Categories:
Transfection 
Authors:
Fox EM, Bernaciak TM, Wen J, Weaver AM, Shupnik MA, Silva CM 
In:
Mol Endocrinol (2008) 22(8): 1781-96 
Categories:
Transfection 
Authors:
Bicaku E, Marchion DC, Schmitt ML, Münster PN 
In:
Cancer Res (2008) 68(5): 1513-9 
Categories:
Transfection 
Authors:
Schrecengost RS, Riggins RB, Thomas KS, Guerrero MS, Bouton AH 
In:
Cancer Res (2007) 67(13): 6174-82 
Categories:
Transfection 
Authors:
Shatnawi A, Tran T, Ratnam M 
In:
Mol Endocrinol (2007) 21(3): 635-50 
Categories:
Transfection 
Authors:
Nagahata T, Sato T, Tomura A, Onda M, Nishikawa K and Emi M 
In:
Endocr Relat Cancer (2005) 12(1): 65-73