cells

osteosarcoma with epithelial morphology

Cell Type:

Bone

Tissue Origin:

bone

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

McCoy's 5A Medium

McCoy's 5A Medium was developed in 1956 to be formulated for the nutritional requirements of Walker 256 carcinoma. Its unique components include asparagine, 3X normal glucose, amino acids (all Lisomers) and increased folic acid. It is a general purpose medium for primary and established cell lines.

12-168 With L-glutamine and 25 mM HEPES
12-688 With L-glutamine

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	EN-137	2e5	76-84%	80-84%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	EN-137	1e6	94%	68-74%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	D-024	1e6	78-86%	74-84%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Identification of Putative Target Genes of the Transcription Factor RUNX2

Categories:

Transfection

Authors:

M Kuhlwilm, A Davierwala, S Pääbo

In:

PLoS ONE (2013) 8 (12): e83218

Saos-2

Recommended Media

Transfection Information

Citations