IMR-90
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Human lung embryonic fibroblast [Nichols WW , et al. Science 196: 60-63, 1977].
Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SE
CM-120
1e6
63.9-67.9%
71-73%
Plasmid (general)
2 µg
100 µl
4D X-Unit
SE
CM-120
2e5
64% ±2
67% ±1
Plasmid (general)
400 ng
20 µl
4D X-Unit
R
X-001
1e6
51% ±3
70% ±3
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Activating c-KIT mutations confer oncogenic cooperativity and rescue RUNX1/ETO-induced DNA damage and apoptosis in human primary CD34+ hematopoietic progenitors.
Categories:
Primary Cells and Media
Authors:
Wichmann C, Quagliano-Lo Coco I, Yildiz Ö, Chen-Wichmann L, Weber H, Syzonenko T, Döring C, Brendel C, Ponnusamy K, Kinner A, Brandts C, Henschler R, Grez M
In:
Leukemia (2015) 29(2): 279-89
RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection
Categories:
Transfection
Authors:
Wang J, Quake SR
In:
Proc Natl Acad Sci USA (2014) 111(36): 13157-62
Unc119 Regulates Myofibroblast Differentiation through the Activation of Fyn and the p38 MAPK Pathway
Categories:
Transfection
Authors:
Vepachedu R, Gorska MM, Singhania N, Cosgrove GP, Brown KK, Alam R
In:
J Immunol (2007) 179(1): 682-90
Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts
Categories:
Transfection
Authors:
Kuang PP, Zhang XH, Rich CB, Foster JA, Subramanian M, Goldstein RH
In:
Am J Physiol Lung Cell Mol Physiol (2007) 292(4): L944-52
Cell divisions are required for L1 retrotransposition
Categories:
Transfection
Authors:
Shi X, Seluanov A, Gorbunova V
In:
Mol Cell Biol (2007) 27(4): 1264-70
BCL2 is a downstream effector of MIZ-1 essential for blocking c-MYC induced apoptosis
Categories:
Transfection
Authors:
Patel JH, McMahon SB
In:
J Biol Chem (2007) 282(1): 5-13