cells

B cell lymphoma cell line derived from a spontaneous reticulum cell neoplasm in BALB/c mouse.

Cell Type:

B cell

Tissue Origin:

blood

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	FF-113	4e5	98%	70-76%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	FF-113	2e6	98%	65-67%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	L-013	2e6	63-85%	72-90%	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	FF-150	4e6	80%	good	Plasmid (general)	2 µg	20 µl	96-well
SF	FF-120	4e6	80%	good	Plasmid (general)	2 µg	20 µl	96-well

Citations

Actin-binding protein 1 regulates B cell receptor-mediated antigen processing and presentation in response to B cell receptor activation

Categories:

Transfection

Authors:

Onabajo OO, Seeley MK, Kale A, Qualmann B, Kessels M, Han J, Tan TH, Song W

In:

J Immunol (2008) 180(10): 6685-95

The MHV68 M2 protein drives IL-10 dependent B cell proliferation and differentiation

Categories:

Transfection

Authors:

Siegel AM, Herskowitz JH, Speck SH

In:

PLoS Pathog (2008) 4(4): e1000039

The rap GTPases regulate B cell morphology, immune-synapse formation, and signaling by particulate B cell receptor ligands

Categories:

Transfection

Authors:

Lin KB, Freeman SA, Zabetian S, Brugger H, Weber M, Lei V, Dang-Lawson M, Tse KW, Santamaria R, Batista FD, Gold MR

In:

Immunity (2008) 28(1): 75-87

A20

Transfection Information

Citations