Raji

Burkitt's lymphoma, B lymphocyte

Cell Type:
B cell
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SG DS-104 4e5 54-76% 66-76% Plasmid (general) 400 ng 20 µl 4D X-Unit
SG DS-104 2e6 63-75% 53-89% Plasmid (general) 2 µg 100 µl 4D X-Unit
V M-013 2e6 52-64% 61-73% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (14)

Categories:
Transfection 
Authors:
Zah E, Lin MY, Silva-Benedict A, Jensen MC, Chen YY 
In:
Other (2016) 4(6): 498-508 
Categories:
Transfection 
Authors:
Wang J, Quake SR 
In:
Proc Natl Acad Sci USA (2014) 111(36): 13157-62 
Categories:
Transfection 
Authors:
Ikeda O, Sekine Y, Yasui T, Oritani K, Sugiyma K, Muromoto R, Ohbayashi N, Yoshimura A, Matsuda T 
In:
Mol Cell Biol (2008) 28(16): 5027-42 
Categories:
Transfection 
Authors:
Majumder P, Gomez JA, Chadwick BP, Boss JM 
In:
J Exp Med (2008) 205(4): 785-98 
Categories:
Transfection 
Authors:
Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM 
In:
J Virol (2007) 81(17): 9346-56 
Categories:
Transfection 
Authors:
De Falco G, Leucci E, Lenze D, Piccaluga PP, Claudio PP, Onnis A, Cerino G, Nyagol J, Mwanda W, Bellan C, Hummel M, Pileri S, Tosi P, Stein H, Giordano A, Leoncini L 
In:
Blood (2007) 110(4): 1301-7 
Categories:
Transfection 
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA 
In:
Oncogene (2007) 26(26): 3797-810 
Categories:
Transfection 
Authors:
Hussain SR, Cheney CM, Johnson AJ, Lin TS, Grever MR, Caligiuri MA, Lucas DM, Byrd JC 
In:
Clin Cancer Res (2007) 13(7): 2144-50 
Categories:
Transfection 
Authors:
Kwon MJ, Soh JW, Chang CH 
In:
J Immunol (2006) 177(2): 950-6 
Categories:
Transfection 
Authors:
Ding Y, Lee JF, Lu H, Lee MH, Yan DH 
In:
Mol Cell Biol (2006) 26(5): 1979-96 
Categories:
Transfection 
Authors:
Hong M, Murai Y, Kutsuna T, Takahashi H, Nomoto K, Cheng CM, Ishizawa S, Zhao QL, Ogawa R, Harmon BV, Tsuneyama K, Takano Y 
In:
J Cancer Res Clin Oncol (2006) 132(1): 1-8 
Categories:
Transfection 
Authors:
Schelcher C, Valencia S, Delecluse HJ, Hicks M and Sinclair AJ 
In:
J Virol (2005) 79(21): 13822-13828 
Categories:
Transfection 
Authors:
Negrete OA, Levroney EL, Aguilar HC, Bertolotti-Ciarlet A, Nazarian R, Tajyar S and Lee B 
In:
Nature (2005) 436(7049): 401-405 
Categories:
Transfection 
Authors:
Kawaida R, Yamada R, Kobayashi K, Tokuhiro S, Suzuki A, Kochi Y, Chang X, Sekine A, Tsunoda T, Sawada T, Furukawa H, Nakamura Y and Yamamoto K 
In:
Genes Immun (2005) 6(3): 194-202 
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