RBL-1
Export
basophilic leukemia cells
Cell Type:
Granulocyte
Tissue Origin:
Species:
rat
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
T-030
1e6
78-88%
54-80%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
DA-104
2e5
65-79%
47-56%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
DA-104
1e6
78-74%
54-60%
Plasmid (general)
2 µg
100 µl
4D X-Unit
Citations
Sustained activation of the tyrosine kinase Syk by antigen in mast cells requires local Ca2+ influx through Ca2+ release-activated Ca2+ (CRAC) channels
Categories:
Transfection
Authors:
Ng SW, Dicapite JL, Singaravelu K, Parekh AB
In:
J Biol Chem (2008) epub: online
Ca2+-calmodulin dependent facilitation and Ca2+ inactivation of Ca2+ release activated Ca2+ channels
Categories:
Transfection
Authors:
Moreau B, Straube S, Fisher RJ, Putney JW, Parekh AB
In:
J Biol Chem (2005) 280(10): 8776-8783