cells

bone marrow; established from a long-term bone marrow culture of BDF1 mice; FDCP = factor dependent cell-Paterson

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

bone marrow

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
R	T-001	1e6	40%	63%	Plasmid (general)	2 µg	100 µl	I/II/2b
L	T-020	2e6	81%	91%	Plasmid (general)	0.5 µg	100 µl	I/II/2b
L	D-017	2e6	93%	64%	Plasmid (general)	0.5 µg	100 µl	I/II/2b

FDCP-Mix

Recommended Media

Transfection Information