cells

Human lung carcinoma established from an explanted lung tumor which was removed from a 58-year-old Caucasian man (Giard DJ , et al. J. Natl. Cancer Inst. (1973) 51: 1417-1423).

Cell Type:

Respiratory Tract Epith.

Tissue Origin:

lung

Species:

human

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CM-130	2e5	79-81%	56-64%	Plasmid (general)	200 ng	20 µl	4D X-Unit
SF	CM-130	1e6	79-83%	60-66%	Plasmid (general)	1 µg	100 µl	4D X-Unit
T	X-001	1e6	69-75%	74-88%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (13)

Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses

Categories:

Transfection

Authors:

Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano KA, Hayashi JM, Carlson-Stevermer J, Zengel JR, Richards CM, Fozouni P, Oki J, Rodriguez L, Joehnk B, Walcott K, Holden K, Sil A, Carette JE, Krogan NJ, Ott M, Puschnik AS

In:

Cell (2021) Jan 7;184(1): 106-119.e14.

Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Powell JD, Hutchison JR, Hess BM, Straub TM

In:

Other (2015) 119(3): 711-23

The role of matrix metalloproteinase in the intimal sarcoma-like cells derived from endarterectomized tissues from a chronic thromboembolic pulmonary hypertension patient.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Jujo T, Sakao S, Tsukahara M, Kantake S, Maruoka M, Tanabe N, Masuda M, Tatsumi K.

In:

PLoS ONE (2014) 9(1): 1371

Efficient gene delivery to primary alveolar epithelial cells by nucleofection.

Categories:

Transfection

Authors:

Grzesik BA, Vohwinkel CU, Morty RE, Mayer K, Herold S, Seeger W, Vadász I.

In:

Am J Physiol Lung Cell Mol Physiol (2013) epub: 103(9)

Histone acetylation and flagellin are essential for Legionella pneumophila-induced cytokine expression

Categories:

Transfection

Authors:

Schmeck B, Lorenz J, N'guessan PD, Opitz B, van Laak V, Zahlten J, Slevogt H, Witzenrath M, Flieger A, Suttorp N, Hippenstiel S

In:

J Immunol (2008) 181(2): 940-7

Ubiquitin-proteasome-mediated degradation of keratin intermediate filaments in mechanically stimulated A549 cells

Categories:

Transfection

Authors:

Jaitovich A, Mehta S, Na N, Ciechanover A, Goldman RD, Ridge KM

In:

J Biol Chem (2008) epub: online

NAIP and Ipaf control Legionella pneumophila replication in human cells

Categories:

Transfection

Authors:

Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B

In:

J Immunol (2008) 180(10): 6808-15

APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks

Categories:

Transfection

Authors:

Iles N, Rulten S, El-Khamisy SF, Caldecott KW

In:

Mol Cell Biol (2007) 27(10): 3793-803

Legionella pneumophila induced IFNbeta in lung epithelial cells via IPS-1 and IRF3 which also control bacterial replication

Categories:

Transfection

Authors:

Opitz B, Vinzing M, van Laak V, Schmeck B, Heine G, Gunther S, Preissner R, Slevogt H, N Guessan PD, Eitel J, Goldmann T, Flieger A, Suttorp N, Hippenstiel S

In:

J Biol Chem (2006) 281(47): 36173-9

Inhibition of the type I interferon response by the nucleoprotein of the prototypic arenavirus lymphocytic choriomeningitis virus

Categories:

Transfection

Authors:

Martinez-Sobrido L, Zuniga EI, Rosario D, Garcia-Sastre A, de la Torre JC

In:

J Virol (2006) 80(18): 9192-9

Sp1/Sp3-dependent regulation of human telomerase reverse transcriptase (hTERT) promoter activity by the bioactive sphingolipid ceramide

Categories:

Transfection

Authors:

Wooten LG and Ogretmen B

In:

J Biol Chem (2005) 280(32): 28867-28876

Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-Cys peroxiredoxins

Categories:

Transfection

Authors:

Woo HA, Jeong W, Chang TS, Park KJ, Park SJ, Yang JS and Rhee SG

In:

J Biol Chem (2005) 280(5): 3125-3128

Characterization of Mammalian Sulfiredoxin and Its Reactivation of Hyperoxidized Peroxiredoxin through Reduction of Cysteine Sulfinic Acid in the Active Site to Cysteine

Categories:

Transfection

Authors:

Chang TS, Jeong W, Woo HA, Lee SM, Park S and Rhee SG

In:

J Biol Chem (2004) 279(49): 50994-51001

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