Epithelial, renal, human (HRE)

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Also called Human kidney epithelial cells (HKEC)Primary epithelial cells isolated from human kidney.

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
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Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Renal Epithelial Cell Growth Medium

REGM BulletKit is formulated to maintain and expand most renal epithelial cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic T-013 5e5 40% 65% Plasmid (general) 3 µg 100 µl I/II/2b
Epithelial, basic T-020 5e5 40% 65% Plasmid (general) 3 µg 100 µl I/II/2b

Citations

Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lednicky JA1, Waltzek TB, McGeehan E, Loeb JC, Hamilton SB, Luetke MC 
In:
Virol J (2013) 10(2013): 10.1186 
Early de novo DNA methylation and prolonged demethylation in the muscle lineage
Categories:
Primary Cells and Media 
Authors:
Tsumagari K1, Baribault C, Terragni J, Varley KE, Gertz J, Pradhan S, Badoo M, Crain CM, Song L, Crawford GE, Myers RM, Lacey M, Ehrlich M. 
In:
Epigenetics (2013) 8(3): 317-32 
Activities of different classes of acyclic nucleoside phosphonates against BK virus in primary human renal cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Topalis D1, Lebeau I, Krecmerová M, Andrei G, Snoeck R 
In:
Antimicrob Agents Chemother (2011) 55(5): 1961-7 
EP2 receptor mediates PGE2-induced cystogenesis of human renal epithelial cells
Categories:
Transfection 
Authors:
Elberg G, Elberg D, Lewis TV, Guruswamy S, Chen L, Logan CJ, Chan MD, Turman MA 
In:
Am J Physiol Renal Physiol (2007) 293(5): F1622-32 

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