HL-60

human acute myeloid leukemia

Cell Type:
Leukocyte (unspec.)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-019 2e6 43% ±3 65% Plasmid (general) 1 µg 100 µl I/II/2b
V T-019 2e6 50% ±1 65% Plasmid (general) 1 µg 100 µl I/II/2b
SF 96-EN-138 4e5 58% ±5 61% ±7 Plasmid (general) 0.4 µg 20 µl Shuttle
SF 96-EN-138 4e5 siRNA 8 ng 20 µl Shuttle
SF 96-EN-138 4e5 siRNA 80 ng 20 µl Shuttle
SF

EN-138

4e5 44.7% ±2 61.4% ±5 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF

EN-138

2e6 48.2% ±1 49.3% ±1 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (33)

Categories:
Transfection 
Authors:
Cooper KM, Bennin DA, Huttenlocher A 
In:
Mol Biol Cell (2008) 19(8): 3180-91 
Categories:
Transfection 
Authors:
Wang J, Lian H, Zhao Y, Kauss MA, Spindel S 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Pivot-Pajot C, Varoqueaux F, de Saint Basile G, Bourgoin SG 
In:
J Immunol (2008) 180(10): 6786-6797 
Categories:
Transfection 
Authors:
Konopleva M, Watt J, Contractor R, Tsao T, Harris D, Estrov Z, Bornmann W, Kantarjian H, Viallet J, Samudio I, Andreeff M 
In:
Cancer Res (2008) 68(9): 3413-20 
Categories:
Transfection 
Authors:
Hackanson B, Bennett KL, Brena RM, Jiang J, Claus R, Chen SS, Blagitko-Dorfs N, Maharry K, Whitman SP, Schmittgen TD, Lübbert M, Marcucci G, Bloomfield CD, Plass C 
In:
Cancer Res (2008) 68(9): 3142-51 
Categories:
Transfection 
Authors:
Youlyouz-Marfak I, Gachard N, Le Clorennec C, Najjar I, Baran-Marszak F, Reminieras L, May E, Bornkamm GW, Fagard R, Feuillard J 
In:
Cell Death Differ (2008) 15(2): 376-85 
Categories:
Transfection 
Authors:
Secchiero P, Zerbinati C, Melloni E, Milani D, Campioni D, Fadda R, Tiribelli M, Zauli G 
In:
Neoplasia (2007) 9(10): 853-61 
Categories:
Transfection 
Authors:
Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A 
In:
Blood (2007) 110(6): 2027-33 
Categories:
Transfection 
Authors:
de la Fuente J, Manzano-Roman R, Blouin EF, Naranjo V, Kocan KM 
In:
BMC Infect Dis (2007) 7(1): 110 
Categories:
Transfection 
Authors:
Chen R, Yang L, McIntyre TM 
In:
J Biol Chem (2007) 282(34): 24842-50 
Categories:
Transfection 
Authors:
von Vietinghoff S, Tunnemann G, Eulenberg C, Wellner M, Cardoso MC, Luft FC, Kettritz R 
In:
Blood (2007) 109(10): 4487-93 
Categories:
Transfection 
Authors:
Pacquelet S, Johnson JL, Ellis BA, Brzezinska AA, Lane WS, Munafo DB, Catz SD 
In:
Biochem J (2007) 403(3): 451-61 
Categories:
Transfection 
Authors:
Bernhagen J, Krohn R, Lue H, Gregory JL, Zernecke A, Koenen RR, Dewor M, Georgiev I, Schober A, Leng L, Kooistra T, Fingerle-Rowson G, Ghezzi P, Kleemann R, McColl SR, Bucala R, Hickey MJ, Weber C 
In:
Nat Med (2007) 13(5): 587-596 
Categories:
Transfection 
Authors:
Li L, Han W, Gu Y, Qiu S, Lu Q, Jin J, Luo J, Hu X 
In:
Cancer Res (2007) 67(10): 4894-903 
Categories:
Primary Cells and Media 
Authors:
Alcantara O, Boldt DH 
In:
Br J of Haematol (2007) 137(3): 252-61 
Categories:
Transfection 
Authors:
Raponi M, Harousseau JL, Lancet JE, Lowenberg B, Stone R, Zhang Y, Rackoff W, Wang Y, Atkins D 
In:
Clin Cancer Res (2007) 13(7): 2254-60 
Categories:
Transfection 
Authors:
Munafo DB, Johnson JL, Ellis BA, Rutschmann S, Beutler B, Catz SD 
In:
Biochem J (2007) 402(2): 229-39 
Categories:
Transfection 
Authors:
Carter JH, Tourtellotte WG 
In:
J Immunol (2007) 178(5): 3038-47 
Categories:
Transfection 
Authors:
Ohnuma-Ishikawa K, Morio T, Yamada T, Sugawara Y, Ono M, Nagasawa M, Yasuda A, Morimoto C, Ohnuma K, Dang NH, Hosoi H, Verdin E, Mizutani S 
In:
Cancer Res (2007) 67(3): 1019-29 
Categories:
Transfection 
Authors:
Seedhouse CH, Hunter HM, Lloyd-Lewis B, Massip AM, Pallis M, Carter GI, Grundy M, Shang S, Russell NH 
In:
Leukemia (2006) 20(12): 2130-2136 
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K 
In:
Mol Cancer Ther (2006) 5(12): 3096-104 
Categories:
Transfection 
Authors:
Weidemann W, Stelzl U, Lisewski U, Bork K, Wanker EE, Hinderlich S, Horstkorte R 
In:
FEBS Lett (2006) 580(28-29): 6649-54 
Categories:
Transfection 
Authors:
Lehman N, Di Fulvio M, McCray N, Campos I, Tabatabaian F, Gomez-Cambronero J 
In:
Blood (2006) 108(10): 3564-72 
Categories:
Transfection 
Authors:
Konopleva M, Contractor R, Tsao T, Samudio I, Ruvolo PP, Kitada S, Deng X, Zhai D, Shi YX, Sneed T, Verhaegen M, Soengas M, Ruvolo VR, McQueen T, Schober WD, Watt JC, Jiffar T, Ling X, Marini FC, Harris D, Dietrich M, Estrov Z, McCubrey J, May WS, Reed JC, Andreeff M 
In:
Cancer Cell (2006) 10(5): 375-88 
Categories:
Transfection 
Authors:
Nakata Y, Tomkowicz B, Gewirtz AM, Ptasznik A 
In:
Blood (2006) 107(11): 4234-9 
Categories:
Transfection 
Authors:
Urahama N, Ito M, Sada A, Yakushijin K, Yamamoto K, Okamura A, Minagawa K, Hato A, Chihara K, Roeder RG and Matsui T 
In:
Genes Cells (2005) 10(12): 1127-1137 
Categories:
Transfection 
Authors:
Balint BL, Szanto A, Madi A, Bauer UM, Gabor P, Benko S, Puskas LG, Davies PJ and Nagy L 
In:
Mol Cell Biol (2005) 25(13): 5648-5663 
Categories:
Transfection 
Authors:
Gruber J, Lampe T, Osborn M and Weber K 
In:
J Cell Sci (2005) 118(Pt 4): 689-696 
Categories:
Transfection 
Authors:
Nagata E, Luo HR, Saiardi A, Bae BI, Suzuki N and Snyder SH 
In:
J Biol Chem (2005) 280(2): 1634-1640 
Categories:
Transfection 
Authors:
Chung MC and Kawamoto S 
In:
J Biol Chem (2004) 279(53): 56042-56052 
Categories:
Transfection 
Authors:
Lacalle RA, Gomez-Mouton C, Barber DF, Jimenez-Baranda S, Mira E, Martinez-A C, Carrera AC, Manes S 
In:
J Cell Sci (2004) 117(Pt 25): 6207-6215 
Categories:
Transfection 
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM 
In:
Nat Med (2004) 10(11): 1187-1189 
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