cells

Human hepatocellular carcinoma cell line

Cell Type:

Liver Epith.

Tissue Origin:

liver

Species:

human

Research Area:

Cancer Research/Cell Biology

Gastroenterology

Toxicology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	EH-100	2e5	72.1% ±5	92%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	EH-100	1e6	68.9% ±2	92%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SF	96-EH-100	2e5	95.5% ±2	92.7% ±11	Plasmid (general)	0.4 µg	20 µl	96-well
V	T-028	1e6	34% ±14	83% ±10	Plasmid (general)	5 µg	100 µl	I/II/2b
V	T-028	1e6	64% ±7	86% ±7	Plasmid (general)	5 µg	100 µl	I/II/2b

Citations (21)

Hepatitis Delta Virus Acts as an Immunogenic Adjuvant in Hepatitis B Virus-Infected Hepatocytes

Categories:

Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Christine Y.L. Tham, Janine Kah, Anthony T. Tan, ..., Marc L€utgehetmann, Maura Dandri, Antonio Bertoletti

In:

Cell Rep (2020) 1 (4): 100060

Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Cell Lines and Primary Cancer Cells

Authors:

Shin-Wei C hen, Misao Himeno, Yuta Koui, Masaya Sugiyama, Hironori Nishitsuji, Masashi Mizokami, Kunitada Shimotohno, Atsushi Miyajima & Taketomo Kido

In:

Scientific Reports (2020) 10: 14349

TGF-ß inhibition via CRISPR promotes the long-term efficacy of CAR T cells against solid tumors

Categories:

Transfection

Authors:

Tang N, Cheng C, Zhang X, Qiao M, Li N, Mu W, Wei XF, Han W, Wang H.

In:

(2020) 27;5(4): e133977

Generation of hepatobiliary organoids from human induced pluripotent stem cells

Categories:

Authors:

Fenfang Wu , Di Wu , Yong Ren , Yuhua Huang , Bo Feng , Nan Zhao , Taotao Zhang , Xiaoni Chen , Shangwu Chen , Anlong Xu

In:

J Clin Transl Hepatol (2019) 70: 1145

A Plasmodium phospholipase is involved in disruption of the liver stage parasitophorous vacuole membrane.

Categories:

Transfection

Authors:

Burda PC, Roelli MA, Schaffner M, Khan SM, Janse CJ, Heussler VT

In:

PLoS Pathog (2015) 11(3): e1004760

Identification of Putative Target Genes of the Transcription Factor RUNX2

Categories:

Transfection

Authors:

M Kuhlwilm, A Davierwala, S Pääbo

In:

PLoS ONE (2013) 8 (12): e83218

Snail depletes the tumorigenic potential of glioblastoma

Categories:

Primary Cells and Media

Authors:

Savary K, Caglayan D, Caja L, Tzavlaki K, Bin Nayeem S, Bergström T, Jiang Y, Uhrbom L, Forsberg-Nilsson K, Westermark B, Heldin CH, Ferletta M, Moustakas

In:

Oncogene (2012) 32(47): 5409-5420

The hereditary hemochromatosis protein, HFE, inhibits iron uptake via downregulation of Zip14 in HEPG2 cells

Categories:

Transfection

Authors:

Gao J, Zhao N, Knutson MD, Enns CA

In:

J Biol Chem (2008) 283(31): 21462-8

Selective Partial Agonism of Liver X Receptor alpha Is Related to Differential Corepressor Recruitment

Categories:

Transfection

Authors:

Phelan CA, Weaver JM, Steger DJ, Joshi S, Maslany JT, Collins JL, Zuercher WJ, Willson TM, Walker M, Jaye M, Lazar MA

In:

Mol Endocrinol (2008) epub: online

Neogenin-mediated hemojuvelin shedding occurs after hemojuvelin traffics to the plamsa membrane

Categories:

Transfection

Authors:

Zhang AS, Yang F, Meyer K, Hernandez C, Chapman-Arvedson T, Bjorkman PJ, Enns CA

In:

J Biol Chem (2008) 283(25): 17494-502

Identification of a novel sn-glycerol-3-phosphate acyltransferase isoform, GPAT4 as the enzyme deficient in Agpat6-/- mice

Categories:

Transfection

Authors:

Nagle CA, Vergnes L, Dejong H, Wang S, Lewin TM, Reue K, Coleman RA

In:

J Lipid Res (2008) 49(4): 823-31

A mouse model for nonsense mutation bypass therapy shows a dramatic multiday response to geneticin

Categories:

Transfection

Authors:

Yang C, Feng J, Song W, Wang J, Tsai B, Zhang Y, Scaringe WA, Hill KA, Margaritis P, High KA, Sommer SS

In:

Proc Natl Acad Sci USA (2007) 104(39): 15394-9

The PPARdelta agonist GW501516 suppresses interleukin-6-mediated hepatocyte acute phase reaction via STAT3 inhibition

Categories:

Transfection

Authors:

Kino T, Rice KC, Chrousos GP

In:

Eur J Clin Invest (2007) 37(5): 425-33

Evidence that inhibition of hemojuvelin shedding in response to iron is mediated through neogenin

Categories:

Transfection

Authors:

Zhang AS, Anderson SA, Meyers KR, Hernandez C, Eisenstein RS, Enns CA

In:

J Biol Chem (2007) 282(17): 12547-56

A cell-penetrating ARF peptide inhibitor of FoxM1 in mouse hepatocellular carcinoma treatment

Categories:

Transfection

Authors:

Gusarova GA, Wang IC, Major ML, Kalinichenko VV, Ackerson T, Petrovic V, Costa RH

In:

J Clin Invest (2007) 117(1): 99-111

Phosphatidylinositol-3-Kinase Regulates Scavenger Receptor Class B Type I Subcellular Localization and Selective Lipid Uptake in Hepatocytes

Categories:

Transfection

Authors:

Shetty S, Eckhardt ER, Post SR, van der Westhuyzen DR

In:

Arterioscler Thromb Vasc Biol (2006) 26(9): 2125-31

The N-CoR complex enables chromatin remodeler SNF2H to enhance repression by thyroid hormone receptor

Categories:

Transfection

Authors:

Alenghat T, Yu J, Lazar MA

In:

EMBO J (2006) 25(17): 3966-74

Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei

Categories:

Transfection

Authors:

Citterio C, Jones HD, Pacheco-Rodriguez G, Islam A, Moss J, Vaughan M

In:

Proc Natl Acad Sci USA (2006) 103(8): 2683-8

Probucol enhances the expression of human hepatic scavenger receptor class B type I, possibly through a species-specific mechanism

Categories:

Transfection

Authors:

Hirano KI, Ikegami C, Tsujii KI, Zhang Z, Matsuura F, Nakagawa-Toyama Y, Koseki M, Masuda D, Maruyama T, Shimomura I, Ueda Y and Yamashita S

In:

Arterioscler Thromb Vasc Biol (2005) 25(11): 2422-2427

Erk Associates with and Primes GSK-3beta for Its Inactivation Resulting in Upregulation of beta-Catenin

Categories:

Transfection

Authors:

Ding Q, Xia W, Liu JC, Yang JY, Lee DF, Xia J, Bartholomeusz G, Li Y, Pan Y, Li Z, Bargou RC, Qin J, Lai CC, Tsai FJ, Tsai CH and Hung MC

In:

Mol Cell (2005) 19(2): 159-170

New promoter mutations in the low-density lipoprotein receptor gene which induce familial hypercholesterolaemia phenotype: molecular and functional analysis

Categories:

Transfection

Authors:

Francova H, Trbusek M, Zapletalova P, Kuhrova V

In:

J Inherit Metab Dis (2004) 27(4): 523-8

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Hep G2

Transfection Information

Citations (21)