cells

myeloma cell line

Cell Type:

B cell

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	D-016	5e6	75%	40%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	G-016	5e6	61%	55%	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	DN-100	3e5	30.3% ±2	60.5% ±4	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	DN-100	1e6	47.9% ±8	51% ±13	Plasmid (general)	2 µg	100 µl	4D X-Unit

Citations (11)

Bortezomib-Resistant Nuclear Factor-kappaB Activity in Multiple Myeloma Cells

Categories:

Transfection

Authors:

Markovina S, Callander NS, O'Connor SL, Kim J, Werndli JE, Raschko M, Leith CP, Kahl BS, Kim K, Miyamoto S

In:

Mol Cancer Res (2008) 6(8): 1356-64

Bone marrow stroma confers resistance to Apo2 ligand/TRAIL in multiple myeloma in part by regulating c-FLIP

Categories:

Transfection

Authors:

Perez LE, Parquet N, Shain K, Nimmanapalli R, Alsina M, Anasetti C, Dalton W

In:

J Immunol (2008) 180(3): 1545-55

MEK1/2 inhibitors potentiate UCN-01 lethality in human multiple myeloma cells through a Bim-dependent mechanism

Categories:

Transfection

Authors:

Pei XY, Dai Y, Tenorio S, Lu J, Harada H, Dent P, Grant S

In:

Blood (2007) 110(6): 2092-101

Small interfering RNA of cyclooxygenase-2 induces growth inhibition and apoptosis independently of Bcl-2 in human myeloma RPMI8226 cells

Categories:

Transfection

Authors:

Li QB, Chen ZC, You Y, Zou P

In:

Acta Pharmacol Sin (2007) 28(7): 1031-6

Nur77 converts phenotype of Bcl-B, an anti-apoptotic protein expressed in plasma cells and myeloma

Categories:

Transfection

Authors:

Luciano F, Krajewska M, Ortiz-Rubio P, Krajewski S, Zhai D, Faustin B, Bruey JM, Bailly-Maitre B, Lichtenstein A, Kumar Kolluri S, Satterthwait AC, Zhang XK, Reed JC

In:

Blood (2007) 109(9): 3849-55

Hedgehog signaling maintains a tumor stem cell compartment in multiple myeloma

Categories:

Transfection

Authors:

Peacock CD, Wang Q, Gesell GS, Corcoran-Schwartz IM, Jones E, Kim J, Devereux WL, Rhodes JT, Huff CA, Beachy PA, Watkins DN, Matsui W

In:

Proc Natl Acad Sci USA (2007) 104(10): 4048-53

Dissecting the Roles of Checkpoint Kinase 1/CDC2 and Mitogen-Activated Protein Kinase Kinase 1/2/Extracellular Signal-Regulated Kinase 1/2 in Relation to 7-Hydroxystaurosporine-Induced Apoptosis in Human Multiple Myeloma Cells

Categories:

Transfection

Authors:

Pei XY, Li W, Dai Y, Dent P, Grant S

In:

Mol Pharmacol (2006) 70(6): 1965-1973

Caspase-12 and caspase-4 are not required for caspase-dependent endoplasmic reticulum stress-induced apoptosis

Categories:

Transfection

Authors:

Obeng EA and Boise LH

In:

J Biol Chem (2005) 280(33): 29578-29587

The FA/BRCA pathway is involved in melphalan-induced DNA interstrand cross-link repair and accounts for melphalan resistance in multiple myeloma cells

Categories:

Transfection

Authors:

Chen Q, Van der Sluis PC, Boulware D, Hazlehurst LA and Dalton WS

In:

Blood (2005) 106(2): 698-705

PML mediates IFN-alpha-induced apoptosis in myeloma by regulating TRAIL induction

Categories:

Transfection

Authors:

Crowder C, Dahle O, Davis RE, Gabrielsen OS and Rudikoff S

In:

Blood (2005) 105(3): 1280-1287

HSP27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance

Categories:

Transfection

Authors:

Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T, Shringharpure R, Burger R, Munshi N, Ohtake Y, Saxena S and Anderson KC

In:

Blood (2003) 102(9): 3379-3386

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