Stellate cell, hepatic, human

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Quiescent vitamin-A-rich liver cells which undergo activation mediated by reactive oxygen species, growth factors and inflammatory cytokines following liver damage.

Cell Type:
Pericytes
Tissue Origin:
liver
Species:
human
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Research Area:
Cancer Research/Cell Biology
Gastroenterology
Toxicology
Cell Characteristics:
Adherent

Recommended Media

Human Stellate Cell Growth Media

MCST250 is a medium optimized for the culture of human hepatic stellate cells.

Storage = - 20 ºC (shelf life is 6 month from date of manufacture)
Storage = 2ºC to 8ºC (shelf life is 4 weeks after thawing)

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations

Genome-wide CRISPR Screening to Identify Drivers of TGF-ß-Induced Liver Fibrosis in Human Hepatic Stellate Cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Shan Yu,*† Matthew Ericson,† Andrea Fanjul,† Derek M. Erion,‡ Maria Paraskevopoulou,‡ Erin N. Smith,† Banumathi Cole,§ Ryan Feaver,§ Corine Holub,† Narender Gavva,† Shane R. Horman,† and Jie Huang† 
In:
Chem Biol (2022) 17: 4 
Functionality of Primary Hepatic Non-Parenchymal Cells in a 3D Spheroid Model and Contribution to Acetaminophen Hepatotoxicity
Categories:
3D Cell Culture , Bioassay  
Authors:
Catherine C Bell , Bhavik Chouhan , Linda C Andersson , Håkan Andersson , James W Dear , Dominic P Williams , Magnus Söderberg  
In:
Arch Toxicol (2020) 94: 1251 
Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts
Categories:
Primary Cells and Media 
Authors:
Shinichiro Horiuchi, Yukie Kuroda, Ryuya Fujii, Su-ryang Kim, Seiichi Ishida 
In:
AATEX (2018) 23 (2): 53-62 
Hepatic stellate cells in liver development, regeneration, and cancer
Categories:
Primary Cells and Media 
Authors:
Chunyue Yin, Kimberley J. Evason, Kinji Asahina, and Didier Y.R. Stainier 
In:
J Clin Invest (2013) 123 (5): 1902-1910 
RACK1, a new ADAM12 interacting protein; contribution to liver fibrogenesis
Categories:
Transfection 
Authors:
Bourd-Boittin K, Le Pabic H, Bonnier D, L'helgoualc'h A, Theret N 
In:
J Biol Chem (2008) epub: online 
Binding of hepatitis C virus envelope protein E2 to CD81 up-regulates MMP-2 in human hepatic stellate cells
Categories:
Transfection 
Authors:
Mazzocca A, Cappadona Sciammetta S, Carloni V, Cosmi L, Annunziato F, Arada T, Abrignani S and Pinzani M 
In:
J Biol Chem (2005) 280(12): 11329-11339 

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