cells

African green monkey kidney cell line; derived from CV-1 by transformation with origin defective SV-40 mutant

Cell Type:

Fibroblast

Tissue Origin:

kidney

Species:

monkey

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	CM-130	2e5	90%	83% ±4	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	CM-130	1e6	89% ±1	86% ±1	Plasmid (general)	2 µg	100 µl	4D X-Unit
R	W-001	1e6	99.3% ±	93.9% ±4	Plasmid (general)	5 µg	100 µl	I/II/2b

Citations (22)

The Role of Phospholipase D (PLD) and Grb2 in Chemotaxis

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Knapek KJ

In:

Other (2008) 0:0: Thesis

Identification of a novel sn-glycerol-3-phosphate acyltransferase isoform, GPAT4 as the enzyme deficient in Agpat6-/- mice

Categories:

Transfection

Authors:

Nagle CA, Vergnes L, Dejong H, Wang S, Lewin TM, Reue K, Coleman RA

In:

J Lipid Res (2008) 49(4): 823-31

The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability

Categories:

Transfection

Authors:

Wang C, Kovanen V, Raudasoja P, Eskelinen S, Pospiech H, Myllylä R

In:

J Cell Mol Med (2008) epub: online

Acid-sensing ion channel-1 contributes to axonal degeneration in autoimmune inflammation of the central nervous system

Categories:

Transfection

Authors:

Friese MA, Craner MJ, Etzensperger R, Vergo S, Wemmie JA, Welsh MJ, Vincent A, Fugger L

In:

Nat Med (2007) 13(12): 1483-9

JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions

Categories:

Transfection

Authors:

Rider L, Shatrova A, Feener EP, Webb L, Diakonova M

In:

J Biol Chem (2007) 282(42): 30985-96

Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy

Categories:

Transfection

Authors:

Liu P, Sudhaharan T, Koh RM, Hwang LC, Ahmed S, Maruyama IN, Wohland T

In:

Biophys J (2007) 93(2): 684-98

Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes

Categories:

Transfection

Authors:

Li F, Papworth M, Minczuk M, Rohde C, Zhang Y, Ragozin S, Jeltsch A

In:

Nucleic Acids Res (2007) 35(1): 100-112

Mitogen- and stress-activated protein kinase 1 is critical for interleukin-1-induced, CREB-mediated, c-fos gene expression in keratinocytes

Categories:

Transfection

Authors:

Schiller M, Bohm M, Dennler S, Ehrchen JM, Mauviel A

In:

Oncogene (2006) 25(32): 4449-57

The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction

Categories:

Transfection

Authors:

Yoshimi R, Yamaji S, Suzuki A, Mishima W, Okamura M, Obana T, Matsuda C, Miwa Y, Ohno S, Ishigatsubo Y

In:

J Immunol (2006) 176(6): 3611-24

Rac1 Leads to Phosphorylation-dependent Increase in Stability of the p66shc Adaptor Protein: Role in Rac1-induced Oxidative Stress

Categories:

Transfection

Authors:

Khanday FA, Yamamori T, Mattagajasingh I, Zhang Z, Bugayenko A, Naqvi A, Santhanam L, Nabi N, Kasuno K, Day BW and Irani K

In:

Mol Biol Cell (2006) 17(1): 122-129

RanBPM is an L1-interacting protein that regulates L1-mediated mitogen-activated protein kinase activation

Categories:

Transfection

Authors:

Cheng L, Lemmon S and Lemmon V

In:

J Neurochem (2005) 94(4): 1102-1110

In vivo and in vitro studies support that a new splicing isoform of OLR1 gene is protective against acute myocardial infarction

Categories:

Transfection

Authors:

Mango R, Biocca S, Del Vecchio F, Clementi F, Sangiuolo F, Amati F, Filareto A, Grelli S, Spitalieri P, Filesi I, Favalli C, Lauro R, Mehta JL, Romeo F and Novelli G

In:

Circ Res (2005) 97(2): 152-158

CD229 (Ly9) Lymphocyte Cell Surface Receptor Interacts Homophilically through Its N-Terminal Domain and Relocalizes to the Immunological Synapse

Categories:

Transfection

Authors:

Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P

In:

J Immunol (2005) 174(11): 7033-7042

Two CD1 genes map to the chicken MHC, indicating that CD1 genes are ancient and likely to have been present in the primordial MHC

Categories:

Transfection

Authors:

Salomonsen J, Sorensen MR, Marston DA, Rogers SL, Collen T, van Hateren A, Smith AL, Beal RK, Skjodt K, Kaufman J

In:

Proc Natl Acad Sci USA (2005) 102(24): 8668-8673

The molecular basis of ferroportin-linked hemochromatosis

Categories:

Transfection

Authors:

De Domenico I, Ward DM, Nemeth E, Vaughn MB, Musci G, Ganz T and Kaplan J

In:

Proc Natl Acad Sci USA (2005) 102(25): 8955-8960

Vertebrate nonmuscle myosin II isoforms rescue small interfering RNA-induced defects in COS-7 cell cytokinesis

Categories:

Transfection

Authors:

Bao J, Jana SS and Adelstein RS

In:

J Biol Chem (2005) 280(20): 19594-19599

Identification of Grb2 As a Novel Binding Partner of the Signaling Lymphocytic Activation Molecule-Associated Protein Binding Receptor CD229

Categories:

Transfection

Authors:

Martin M, Del Valle JM, Saborit I and Engel P

In:

J Immunol (2005) 174(10): 5977-5986

Human FK506 binding protein 65 is associated with colorectal cancer

Categories:

Transfection

Authors:

Olesen SH, Christensen LL, Sorensen FB, Cabezon T, Laurberg S, Orntoft TF and Birkenkamp-Demtroder K

In:

Mol Cell Proteomics (2005) 4(4): 534-544

The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration

Categories:

Transfection

Authors:

Pratt SJ, Epple H, Ward M, Feng Y, Braga VM and Longmore GD

In:

J Cell Biol (2005) 168(5): 813-824

Bruton's tyrosine kinase: cell biology, sequence conservation, mutation spectrum, siRNA modifications, and expression profiling

Categories:

Transfection

Authors:

Lindvall JM, Blomberg KE, Valiaho J, Vargas L, Heinonen JE, Berglof A, Mohamed AJ, Nore BF, Vihinen M and Smith CI

In:

Immunol Rev (2005) 203: 200-215

Interleukin-2-induced survival of natural killer (NK) cells involving phosphatidylinositol-3 kinase-dependent reduction of ceramide through acid sphingomyelinase, sphingomyelin synthase and glucosylceramide synthase

Categories:

Transfection

Authors:

Taguchi Y, Kondo T, Watanabe M, Miyaji M, Umehara H, Kotutumi Y, Okazaki T

In:

Blood (2004) 104(10): 3285-3293

Molecular cloning and expression regulation of PRG-3, a new member of the plasticity-related gene family

Categories:

Transfection

Authors:

Savaskan NE, Brauer AU and Nitsch R

In:

Eur J Neurosci (2004) 19(1): 212-220

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