HeLa

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cervical carcinoma (diagnosis later changed to adenocarcinoma) from 31 year old black woman, epitheloid

Cell Type:
Uterine/Vaginal Epith.
Tissue Origin:
cervix
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CN-114 5e4 84.9% ±1 80.9% ±9 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CN-114 3e5 83.6% ±1 83% ±12 Plasmid (general) 2 µg 100 µl 4D X-Unit
SE 96-CN-114 2e5 75% ±6 89% ±2 Plasmid (general) 0.4 µg 20 µl 96-well
R I-013 5e5 20% ±2 85-90% Plasmid (general) 2 µg 100 µl I/II/2b
R I-013 5e5 70% ±3 85-90% Plasmid (general) 2 µg 100 µl I/II/2b
R A-028 1e6 13% ±7 95% Plasmid (general) 5 µg 100 µl I/II/2b
R A-028 1e6 57% ±7 95% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (45)

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
Categories:
Transfection 
Authors:
Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grünewald J, Joung JK 
In:
Nat Biotechnol (2021) Jan;39(1): 41-46 
Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing
Categories:
Transfection 
Authors:
Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS 
In:
Cell Res (2021) 184(9): 2503-2519.e17 
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
Categories:
Transfection 
Authors:
Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR. 
In:
Scientific Reports (2021) 11(1): 19482 
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Categories:
Transfection 
Authors:
Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE 
In:
Nat Commun. (2020) 11(1): 2109 
Search-and-replace genome editing without double-strand breaks or donor DNA.
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Anzalone AV1,2,3, Randolph PB1,2,3, Davis JR1,2,3, Sousa AA1,2,3, Koblan LW1,2,3, Levy JM1,2,3, Chen PJ1,2,3, Wilson C1,2,3, Newby GA1,2,3, Raguram A1,2,3, Liu DR4,5,6. 
In:
Nature (2019) 10: 1038 
NK cells switch from granzyme B to death receptor-mediated cytotoxicity during serial killing.
Categories:
Transfection 
Authors:
Prager I, Liesche C, van Ooijen H, Urlaub D, Verron Q, Sandström N, Fasbender F, Claus M, Eils R, Beaudouin J, Önfelt B, Watzl C. 
In:
J Exp Med (2019) 216(9): 2113-2127 
A platform for rapid prototyping of synthetic gene networks in mammalian cells
Categories:
Transfection 
Authors:
Duportet X, Wroblewska L, Guye P, Li Y, Eyquem J, Rieders J, Rimchala T, Batt G, Weiss R. 
In:
Nucleic Acids Res (2014) 42(21): 13440-51 
Recruitment of folliculin to lysosomes supports the amino acid–dependent activation of Rag GTPases
Categories:
Transfection 
Authors:
Constance S. Petit, Agnes Roczniak-Ferguson, and Shawn M. Ferguson 
In:
J Cell Biol (2013) 202(7): 1107-1122 
Efficient oligonucleotide-mediated degradation of nuclear noncoding RNAs in mammalian cultured cells
Categories:
Transfection 
Authors:
Ideue T, Hino K, Kitao S, Yokoi T, Hirose T 
In:
RNA (2009) 15(8): 1578-1587 
Multiple Regulatory Steps Control Mammalian Nonmuscle Myosin II Assembly in Live Cells
Categories:
Transfection 
Authors:
Breckenridge MT, Dulyaninova NG, Egelhoff TT 
In:
Mol Biol Cell (2008) epub: online 
The mechanism of micro-RNA-mediated translation repression is determined by the promoter of the target gene
Categories:
Transfection 
Authors:
Kong YW, Cannell IG, de Moor CH, Hill K, Garside PG, Hamilton TL, Meijer HA, Dobbyn HC, Stoneley M, Spriggs KA, Willis AE, Bushell M 
In:
Proc Natl Acad Sci USA (2008) 105(26): 8866-8871 
HIV-1 Vpr binds to the N-lobe of Wee1 kinase domain and enhances kinase activity for Cdc2
Categories:
Transfection 
Authors:
Kamata M, Watanabe N, Nagaoka Y, Chen IS 
In:
J Virol (2008) 82(12): 5672-82 
The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment
Categories:
Transfection 
Authors:
Stumpff J, von Dassow G, Wagenbach M, Asbury C, Wordeman L 
In:
Dev Cell (2008) 14(2): 252-62 
The C terminus of the synovial sarcoma-associated SSX proteins interacts with the LIM homeobox protein LHX4
Categories:
Transfection 
Authors:
de Bruijn DR, van Dijk AH, Willemse MP, van Kessel AG 
In:
Oncogene (2008) 27(5): 653-62 
Peptide-Mediated Disruption of NFB/NRF Interaction Inhibits IL-8 Gene Activation by IL-1 or Helicobacter pylori
Categories:
Transfection 
Authors:
Bartels M, Schweda AT, Dreikhausen U, Frank R, Resch K, Beil W, Nourbakhsh M 
In:
J Immunol (2007) 179(11): 7605-13 
Type I phosphatidylinositol-4,5-bisphosphate 4-phosphatase regulates stress-induced apoptosis
Categories:
Transfection 
Authors:
Zou J, Marjanovic J, Kisseleva MV, Wilson M, Majerus PW 
In:
Proc Natl Acad Sci USA (2007) 104(43): 16834-9 
The proline-Rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mTOR complex 1
Categories:
Transfection 
Authors:
Oshiro N, Takahashi R, Yoshino KI, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K 
In:
J Biol Chem (2007) 282(28): 20329-39 
Live-cell imaging reveals sequential oligomerization and local plasma membrane targeting of stromal interaction molecule 1 after Ca2+ store depletion
Categories:
Transfection 
Authors:
Liou J, Fivaz M, Inoue T, Meyer T 
In:
Proc Natl Acad Sci USA (2007) 104(22): 9301-6 
Elevated Expression of the Oncogene c-fms and Its Ligand, the Macrophage Colony-Stimulating Factor-1, in Cervical Cancer and the Role of Transforming Growth Factor-1 in Inducing c-fms Expression
Categories:
Transfection 
Authors:
Kirma N, Hammes LS, Liu YG, Nair HB, Valente PT, Kumar S, Flowers LC, Tekmal RR 
In:
Cancer Res (2007) 67(5): 1918-26 
A Conserved Dileucine Motif Mediates Clathrin and AP-2-dependent Endocytosis of the HIV-1 Envelope Protein
Categories:
Transfection 
Authors:
Byland R, Vance PJ, Hoxie JA, Marsh M 
In:
Mol Biol Cell (2007) 18(2): 414-25 
Characterization and Functional Consequences of Underexpression of Clusterin in Rheumatoid Arthritis
Categories:
Transfection 
Authors:
Devauchelle V, Essabbani A, De Pinieux G, Germain S, Tourneur L, Mistou S, Margottin-Goguet F, Anract P, Migaud H, Le Nen D, Lequerre T, Saraux A, Dougados M, Breban M, Fournier C, Chiocchia G 
In:
J Immunol (2006) 177(9): 6471-6479 
Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins
Categories:
Transfection 
Authors:
Lyst MJ, Nan X, Stancheva I 
In:
EMBO J (2006) 25(22): 5317-5328 
Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules
Categories:
Transfection 
Authors:
Leung AK, Calabrese JM, Sharp PA 
In:
Proc Natl Acad Sci USA (2006) 103(48): 18125-30 
Tetraspanins CD9 and CD81 Modulate HIV-1-Induced Membrane Fusion
Categories:
Transfection 
Authors:
Gordon-Alonso M, Yanez-Mo M, Barreiro O, Alvarez S, Munoz-Fernandez MA, Valenzuela-Fernandez A, Sanchez-Madrid F 
In:
J Immunol (2006) 177(8): 5129-37 
Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia
Categories:
Transfection 
Authors:
Mukhopadhyay SS, Leung KS, Hicks MJ, Hastings PJ, Youssoufian H, Plon SE 
In:
J Cell Biol (2006) 175(2): 225-35 
Nucleophosmin is essential for ribosomal protein L5 nuclear export
Categories:
Transfection 
Authors:
Yu Y, Maggi LB Jr, Brady SN, Apicelli AJ, Dai MS, Lu H, Weber JD 
In:
Mol Cell Biol (2006) 26(10): 3798-809 
Interrelated roles for Mcl-1 and Bim in regulation of TRAIL-mediated mitochondrial apoptosis
Categories:
Transfection 
Authors:
Han J, Goldstein LA, Gastman BR, Rabinowich H 
In:
J Biol Chem (2006) 281(15): 10153-63 
Nanomolar inhibitors of the peptidyl prolyl cis/trans isomerase Pin1 from combinatorial peptide libraries
Categories:
Transfection 
Authors:
Wildemann D, Erdmann F, Alvarez BH, Stoller G, Zhou XZ, Fanghänel J, Schutkowski M, Lu KP, Fischer G 
In:
J Med Chem (2006) 49(7): 2147-50 
Clathrin Interaction and Subcellular Localization of Ce-DAB-1, an Adaptor for Protein Secretion in Caenorhabditis elegans
Categories:
Transfection 
Authors:
Kamikura DM, Cooper JA 
In:
Traffic (2006) 7(3): 324-36 
hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay
Categories:
Transfection 
Authors:
Wittmann J, Hol EM and Jack HM 
In:
Mol Cell Biol (2006) 26(4): 1272-1287 
The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
EGF stimulates annexin 1-dependent inward vesiculation in a multivesicular endosome subpopulation
Categories:
Transfection 
Authors:
White IJ, Bailey LM, Aghakhani MR, Moss SE and Futter CE 
In:
EMBO J (2006) 25(1): 1-12 
A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptake
Categories:
Transfection 
Authors:
Devireddy LR, Gazin C, Zhu X, Green MR 
In:
Cell (2005) 123(7): 1293-305 
Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters
Categories:
Transfection 
Authors:
Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L and Helenius A 
In:
J Cell Biol (2005) 170(5): 769-779 
Bcl-2 regulator FKBP38 is activated by Ca(2+)/calmodulin
Categories:
Transfection 
Authors:
Edlich F, Weiwad M, Erdmann F, Fanghanel J, Jarczowski F, Rahfeld JU und Fischer G 
In:
EMBO J (2005) 24(14): 2688-2699 
Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes
Categories:
Transfection 
Authors:
Vonderheit A, Helenius A 
In:
PLoS Biol (2005) 3(7): e233 
Degradation of Cdt1 during S phase is Skp2-independent and is required for efficient progression of mammalian cells through S phase
Categories:
Transfection 
Authors:
Takeda DY, Parvin JD and Dutta A 
In:
J Biol Chem (2005) 280(24): 23416-23423 
The molecular basis of ferroportin-linked hemochromatosis
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, Nemeth E, Vaughn MB, Musci G, Ganz T and Kaplan J 
In:
Proc Natl Acad Sci USA (2005) 102(25): 8955-8960 
Regulation of monocyte apoptosis by the protein kinase C delta (PKCdelta )-dependent phosphorylation of caspase-3
Categories:
Transfection 
Authors:
Voss OH, Kim S, Wewers MD and Doseff AI 
In:
J Biol Chem (2005) 280(17): 17371-17379 
Fzo1, a protein involved in mitochondrial fusion, inhibits apoptosis
Categories:
Transfection 
Authors:
Sugioka R, Shimizu S and Tsujimoto Y 
In:
J Biol Chem (2004) 279(50): 52726-52734 
Reversible oxidation and inactivation of the tumor suppressor PTEN in cells stimulated with peptide growth factors
Categories:
Transfection 
Authors:
Kwon J, Lee SR, Yang KS, Ahn Y, Kim YJ, Stadtman ER and Rhee SG 
In:
Proc Natl Acad Sci USA (2004) 101(47): 16419-16424 
Peroxiredoxin III, a Mitochondrion-specific Peroxidase, Regulates Apoptotic Signaling by Mitochondria
Categories:
Transfection 
Authors:
Chang TS, Cho CS, Park S, Yu S, Kang SW and Rhee SG 
In:
J Biol Chem (2004) 279(40): 41975-41984 
Role for Rab7 in maturation of late autophagic vacuoles
Categories:
Transfection 
Authors:
Jager S, Bucci C, Tanida I, Ueno T, Kominami E, Saftig P and Eskelinen EL 
In:
J Cell Sci (2004) 117(Pt 20): 4837-4848 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Categories:
Primary Cells and Media 
Authors:
Peng Zhou,#1 Xing-Lou Yang,#1 Xian-Guang Wang,#2 Ben Hu,1 Lei Zhang,1 Wei Zhang,1 Hao-Rui Si,1,3 Yan Zhu,1 Bei Li,1 Chao-Lin Huang,2 Hui-Dong Chen,2 Jing Chen,1,3 Yun Luo,1,3 Hua Guo,1,3 Ren-Di Jiang,1,3 Mei-Qin Liu,1,3 Ying Chen,1,3 Xu-Rui Shen,1,3 Xi Wang,1,3 Xiao-Shuang Zheng,1,3 Kai Zhao,1,3 Quan-Jiao Chen,1 Fei Deng,1 Lin-Lin Liu,4 Bing Yan,1 Fa-Xian Zhan,4 Yan-Yi Wang,1 Geng-Fu Xiao,1 and Zheng-Li Shi1 
In:
Nature () 579: 7798 
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