293T

Human embryonic kidney (HEK) cells that express SV40 large T antigen

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Pro293 Chemically Defined, Serum-free Media were optimized to support high-density growth and recombinant protein production in 293 human kidney cells. They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal-origin components.

Pro293s Medium - for 293 cells growing in suspension culture
Pro293a Medium - for 293 cells growing in adherent culture


Storage = 2°C to 8°C

12-765 - Pro293s Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)

12-764 - Pro293a Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)
BE02-025Q - Pro293a Medium (without L-glutamine, galaktose or phenol red, with 0.1% Pluronic F-68)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V Q-001 2e6 90% 90% Plasmid (general) 5 µg 100 µl I/II/2b
SF CM-130 2e5 40-50% Plasmid (general) 400 ng 20 µl Shuttle
SF DG-130 2e5 95-100% Plasmid (general) 400 ng 20 µl Shuttle
SF DS-150 2e5 95-100% Plasmid (general) 400 ng 20 µl Shuttle

Citations (24)

Categories:
Transfection 
Authors:
Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganière J, Doyon Y. 
In:
Nat Methods (2017) 14(6): 615-620 
Categories:
Transfection 
Authors:
Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. 
In:
Cell (2016) 167(1): 219-232 
Categories:
Primary Cells and Media 
Authors:
Wichmann C, Quagliano-Lo Coco I, Yildiz Ö, Chen-Wichmann L, Weber H, Syzonenko T, Döring C, Brendel C, Ponnusamy K, Kinner A, Brandts C, Henschler R, Grez M 
In:
Leukemia (2015) 29(2): 279-89 
Categories:
Transfection 
Authors:
Lin S, Staahl BT, Alla RK, Doudna JA. 
In:
eLife (2014) 3: e04766 
Categories:
Transfection 
Authors:
Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS, Stortchevoi A, Bryder D, Musunuru K, Brand H, Tager AM, Allen TM, Talkowski ME, Rossi DJ, Cowan CA 
In:
Cell Stem Cell (2014) 15(5): 643-652 
Categories:
Primary Cells and Media 
Authors:
Suzuki T, Nakano-Ikegaya M, Yabukami-Okuda H, de Hoon M, Severin J, Saga-Hatano S, Shin JW, Kubosaki A, Simon C, Hasegawa Y, Hayashizaki Y, Suzuki H. 
In:
PLoS ONE (2012) 7(3): ePub 
Categories:
Primary Cells and Media 
Authors:
Wahl-Jensen V, Kurz S, Feldmann F, Buehler LK, Kindrachuk J, DeFilippis V, da Silva Correia J, Früh K, Kuhn JH, Burton DR, Feldmann H. 
In:
Other (2011) 5(10): ePub 
Categories:
Primary Cells and Media 
Authors:
Sadvakassova G, Dobocan MC, Congote LF 
In:
Brain Res (2009) 2(215): 1-7 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizo A, Olthof S, Han L, Vellenga E, de Haan G, Schuringa JJ 
In:
Blood (2009) 114(8): 1498-505 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizo A, Dontje B, Vellenga E, de Haan G, Schuringa JJ 
In:
Blood (2008) 111(5): 2621-30 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schepers H, van Gosliga D, Wierenga AT, Eggen BJ, Schuringa JJ, Vellenga E 
In:
Blood (2007) 110(8): 2880-8 
Categories:
Transfection 
Authors:
Appukuttan B, McFarland TJ, Davies MH, Atchaneeyasakul LO, Zhang Y, Babra B, Pan Y, Rosenbaum JT, Acott T, Powers MR, Stout JT 
In:
Invest Ophthalmol Vis Sci (2007) 48(8): 3775-82 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schepers H, Wierenga AT, van Gosliga D, Eggen BJ, Vellenga E, Schuringa JJ 
In:
Blood (2007) 110(4): 1317-25 
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, Langelier C, Vaughn MB, Nemeth E, Sundquist WI, Ganz T, Musci G, Kaplan J 
In:
Mol Biol Cell (2007) 18(7): 2569-78 
Categories:
Transfection 
Authors:
Zohn IE, De Domenico I, Pollock A, Ward DM, Goodman JF, Liang X, Sanchez AJ, Niswander L, Kaplan J 
In:
Blood (2007) 109(10): 4174-80 
Categories:
Transfection 
Authors:
Schupp M, Curtin JC, Kim RJ, Billin AN, Lazar MA 
In:
Mol Pharmacol (2007) 71(5): 1251-7 
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, Musci G, Kaplan J 
In:
Blood (2007) 109(5): 2205-9 
Categories:
Transfection 
Authors:
Wang X, Trotman LC, Koppie T, Alimonti A, Chen Z, Gao Z, Wang J, Erdjument-Bromage H, Tempst P, Cordon-Cardo C, Pandolfi PP, Jiang X 
In:
Cell (2007) 128(1): 129-39 
Categories:
Transfection 
Authors:
Razi M, Futter CE 
In:
Mol Biol Cell (2006) 17(8): 3469-83 
Categories:
Transfection 
Authors:
Michaux G, Pullen TJ, Haberichter SL and Cutler DF 
In:
Blood (2006) 107(10): 3922-4 
Categories:
Transfection 
Authors:
Trakul N, Menard RE, Schade GR, Qian Z and Rosner MR 
In:
J Biol Chem (2005) 280(26): 24931-24940 
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, Nemeth E, Vaughn MB, Musci G, Ganz T and Kaplan J 
In:
Proc Natl Acad Sci USA (2005) 102(25): 8955-8960 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F. 
In:
Mol Ther (2005) 11(1): 120-131 
Categories:
Transfection 
Authors:
Mukhopadhyay S, Clark DR, Watson NB, Zacharias W and McGregor WGx 
In:
Nucleic Acids Res (2004) 32(19): 5820-5826 
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