NCI-H929 [H929]

multiple myeloma

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T A-023 5e6 12% 78% Plasmid (general) 1 µg 100 µl I/II/2b
T T-016 5e6 28% 49% Plasmid (general) 1 µg 100 µl I/II/2b
T O-017 5e6 18% 68% Plasmid (general) 1 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Carew JS, Nawrocki ST, Reddy VK, Bush D, Rehg JE, Goodwin A, Houghton JA, Casero RA Jr, Marton LJ, Cleveland JL 
In:
Cancer Res (2008) 68(12): 4783-90 
Categories:
Transfection 
Authors:
Peacock CD, Wang Q, Gesell GS, Corcoran-Schwartz IM, Jones E, Kim J, Devereux WL, Rhodes JT, Huff CA, Beachy PA, Watkins DN, Matsui W 
In:
Proc Natl Acad Sci USA (2007) 104(10): 4048-53 
Categories:
Transfection 
Authors:
Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio J, Bergsagel L, Stewart AK 
In:
Blood (2006) 108(10): 3465-71 
Categories:
Transfection 
Authors:
Derksen PWB, Tjin E, Meijer HP, Klok MD, Mac Gillavry HD, van Oers MHJ, Lokhorst HM, Bloem AC, Clevers H, Nusse R, van der Neut R, Spaargaren M and Pals ST 
In:
Proc Natl Acad Sci USA (2004) 101(16): 6122-6127 
Categories:
Transfection 
Authors:
Hurt EM, Wiestner A, Rosenwald A, Shaffer AL, Campo E, Grogan T, Bergsagel PL, Kuehl WM and Staudt LM 
In:
Cancer Cell (2004) 5: 191-199