293

Also called HEK293. Embryonic kidney cells transformed by adenovirus type 5

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Pro293 Chemically Defined, Serum-free Media were optimized to support high-density growth and recombinant protein production in 293 human kidney cells. They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal-origin components.

Pro293s Medium - for 293 cells growing in suspension culture
Pro293a Medium - for 293 cells growing in adherent culture


Storage = 2°C to 8°C

12-765 - Pro293s Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)

12-764 - Pro293a Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)
BE02-025Q - Pro293a Medium (without L-glutamine, galaktose or phenol red, with 0.1% Pluronic F-68)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V

A-023

1e6 36% ±12


Plasmid (general) 1 µg 100 µl I/II/2b
V A-023 1e6 57% ±16 Plasmid (general) 1 µg 100 µl I/II/2b
SF

CM-130

2e5 92.7% ±1 88.7% ±5 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF

CM-130

1e6 88.6% ±5 72.6% ±5 Plasmid (general) 2 µg 100 µl 4D X-Unit
V

Q-001

1e6 81% ±5


Plasmid (general) 5 µg 100 µl I/II/2b
V Q-001 1e6 84% ±1 Plasmid (general) 5 µg 100 µl I/II/2b
SF 96-CM-130 2e5 83% ±9 93% ±4 Plasmid (general) 0.4 µg 20 µl Shuttle

Citations (43)

Categories:
Primary Cells and Media, Transfection 
Authors:
Vakulskas CA, Dever DP, Rettig GR, Turk R, Jacobi AM, Collingwood MA, Bode NM, McNeill MS, Yan S, Camarena J, Lee CM, Park SH, Wiebking V, Bak RO, Gomez-Ospina N, Pavel-Dinu M, Sun W6, Bao G, Porteus MH, Behlke MA 
In:
Nat Med (2018) 24(8): 1216-1224 
Categories:
Transfection 
Authors:
Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE. 
In:
Nat Biotechnol (2016) 1(1): 1-7 
Categories:
Transfection 
Authors:
Semaan M, Ivanusic D, Denner J. 
In:
PLoS ONE (2015) 10(4): 1-18 
Categories:
Primary Cells and Media, Classical Media and Reagents 
Authors:
Barr TP, Kornberg D, Montmayeur JP, Long M, Reichheld S, Strichartz GR. 
In:
Anal Biochem (2014) ePub: ePub 
Categories:
Transfection 
Authors:
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F 
In:
Nat Protocols (2013) 8(11): 2281–2308 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Categories:
Primary Cells and Media 
Authors:
Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K 
In:
Cell Adh Migr (2013) 7(5): 426-33 
Categories:
Transfection 
Authors:
Lui-Roberts WW, Ferraro F, Nightingale TD, Cutler DF 
In:
Mol Biol Cell (2008) epub: online 
Categories:
Transfection 
Authors:
Lai JP, Lai S, Tuluc F, Tansky MF, Kilpatrick LE, Leeman SE, Douglas SD 
In:
Proc Natl Acad Sci USA (2008) 105(34): 12605-10 
Categories:
Transfection 
Authors:
Saliba RS, Pangalos M, Moss SJ 
In:
J Biol Chem (2008) 283(27): 18538-44 
Categories:
Transfection 
Authors:
Zhang SL, Kozak JA, Jiang W, Yeromin AV, Chen J, Yu Y, Penna A, Shen W, Chi V, Cahalan MD 
In:
J Biol Chem (2008) 283(25): 17662-71 
Categories:
Transfection 
Authors:
Padmanabhan A, Liu S, Song Z 
In:
Biotechnol Lett (2008) 30(5): 819-27 
Categories:
Transfection 
Authors:
Chanrion B, Mannoury la Cour C, Gavarini S, Seimandi M, Vincent L, Pujol JF, Bockaert J, Marin P, Millan MJ 
In:
Mol Pharmacol (2008) 73(3): 748-57 
Categories:
Transfection 
Authors:
Mignen O, Thompson JL, Shuttleworth TJ 
In:
J Physiol (2008) 586(1): 185-95 
Categories:
Transfection 
Authors:
Kreis S, Philippidou D, Margue C, Rolvering C, Haan C, Dumoutier L, Renauld JC, Behrmann I 
In:
PLoS ONE (2007) 2(12): e1300 
Categories:
Transfection 
Authors:
Lombardi F, Gullotta F, Columbaro M, Filareto A, D'Adamo M, Vielle A, Guglielmi V, Nardone AM, Azzolini V, Grosso E, Lattanzi G, D'Apice MR, Masala S, Maraldi NM, Sbraccia P, Novelli G 
In:
J Clin Endocrinol Metab (2007) 92(11): 4467-71 
Categories:
Transfection 
Authors:
van den Biggelaar M, Bierings R, Storm G, Voorberg J, Mertens K 
In:
J Thromb Haemost (2007) 5(11): 2235-42 
Categories:
Transfection 
Authors:
Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM 
In:
J Virol (2007) 81(17): 9346-56 
Categories:
Transfection 
Authors:
Kaether C, Scheuermann J, Fassler M, Zilow S, Shirotani K, Valkova C, Novak B, Kacmar S, Steiner H, Haass C 
In:
EMBO Rep (2007) 8(8): 743-748 
Categories:
Transfection 
Authors:
Kim D, Rath O, Kolch W, Cho KH 
In:
Oncogene (2007) 26(31): 4571-9 
Categories:
Transfection 
Authors:
Oshiro N, Takahashi R, Yoshino KI, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K 
In:
J Biol Chem (2007) 282(28): 20329-39 
Categories:
Transfection 
Authors:
Bush CF, Jones SV, Lyle AN, Minneman KP, Ressler KJ, Hall RA 
In:
J Biol Chem (2007) 282(26): 19042-51 
Categories:
Transfection 
Authors:
Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, DÜ­az-Gonzalez F 
In:
J Immunol (2007) 178(12): 8053-63 
Categories:
Transfection 
Authors:
Kluiver J, van den Berg A, de Jong D, Blokzijl T, Harms G, Bouwman E, Jacobs S, Poppema S, Kroesen BJ 
In:
Oncogene (2007) 26(26): 3769-76 
Categories:
Transfection 
Authors:
Chanrion B, Mannoury la Cour C, Bertaso F, Lerner-Natoli M, Freissmuth M, Millan MJ, Bockaert J, Marin P 
In:
Proc Natl Acad Sci USA (2007) 104(19): 8119-24 
Categories:
Transfection 
Authors:
Mignen O, Thompson JL, Shuttleworth TJ 
In:
J Physiol (2007) 579(Pt 3): 703-15 
Categories:
Transfection 
Authors:
Barfod ET, Moore AL, Roe MW, Lidofsky SD 
In:
J Biol Chem (2007) 282(12): 8984-93 
Categories:
Transfection 
Authors:
Prinster SC, Holmqvist TG, Hall RA 
In:
J Pharmacol Exp Ther (2006) 318(3): 974-81 
Categories:
Transfection 
Authors:
Schieke SM, Phillips D, McCoy JP Jr, Aponte AM, Shen RF, Balaban RS, Finkel T 
In:
J Biol Chem (2006) 281(37): 27643-52 
Categories:
Transfection 
Authors:
Thompson AJ, Padgett CL, Lummis SC 
In:
J Biol Chem (2006) 281(24): 16576-82 
Categories:
Transfection 
Authors:
Michaux G, Pullen TJ, Haberichter SL and Cutler DF 
In:
Blood (2006) 107(10): 3922-4 
Categories:
Transfection 
Authors:
Nofer JR, Remaley AT, Feuerborn R, Wolinska I, Engel T, von Eckardstein A and Assmann G 
In:
J Lipid Res (2006) 47(4): 794-803 
Categories:
Transfection 
Authors:
Haddad D, Maciel J, Kumar N 
In:
Infect Immun (2006) 74(4): 2043-51 
Categories:
Transfection 
Authors:
Michaux G, Abbitt KB, Collinson LM, Haberichter SL, Norman KE, Cutler DF 
In:
Dev Cell (2006) 10(2): 223-32 
Categories:
Transfection 
Authors:
Lui-Roberts WW, Collinson LM, Hewlett LJ, Michaux G and Cutler DF 
In:
J Cell Biol (2005) 170(4): 627-636 
Categories:
Transfection 
Authors:
von Arnim CA, Kinoshita A, Peltan ID, Tangredi MM, Herl L, Lee BM, Spoelgen R, Hshieh TT, Ranganathan S, Battey FD, Liu CX, Bacskai BJ, Sever S, Irizarry MC, Strickland DK and Hyman BT 
In:
J Biol Chem (2005) 280(18): 17777-17785 
Categories:
Transfection 
Authors:
Mead KI, Zheng Y, Manzotti CN, Perry LC, Liu MK, Burke F, Powner DJ, Wakelam MJ and Sansom DM 
In:
J Immunol (2005) 174(8): 4803-4811 
Categories:
Transfection 
Authors:
Muresan Z and Muresan V 
In:
J Neurosci (2005) 25(15): 3741-3751 
Categories:
Transfection 
Authors:
Chen YC, Pohl G, Wang TL, Morin PJ, Risberg B, Kristensen GB, Yu A, Davidson B and Shih IeM 
In:
Cancer Res (2005) 65(1): 331-337 
Categories:
Transfection 
Authors:
Maniura-Weber K, Goffart S, Garstka HL, Montoya J and Wiesner RJ 
In:
Nucleic Acids Res (2004) 32(20): 6015-6027 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Categories:
Transfection 
Authors:
Hayashi M, Kim SW, Imanaka-Yoshida K, Yoshida T, Abel ED, Eliceiri B, Yang Y, Ulevitch RJ and Lee JD 
In:
J Clin Invest (2004) 113(8): 1138-48 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109 
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