HT-29

Export

colorectal adenocarcinoma

Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R W-017 5e5 40-60% 47-67% Plasmid (general) 2 µg 100 µl I/II/2b
R W-017 5e5 33-49% 40-74% Plasmid (general) 2 µg 100 µl I/II/2b
SF FF-137 1e6 50-52% 60% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF FF-137 2e5 66-68% 60% Plasmid (general) 400 ng 20 µl 4D X-Unit

Citations (10)

Cancer invasion regulates vascular complexity in a three-dimensional biomimetic model.
Categories:
3D Cell Culture  
Authors:
Pape J1, Magdeldin T1, Ali M2, Walsh C2, Lythgoe M2, Emberton M3, Cheema U4. 
In:
Eur J Cell Biol (2019) 119: 193 
Commensal gut bacteria modulate phosphorylation-dependent PPAR? transcriptional activity in human intestinal epithelial cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nepelska M, de Wouters T, Jacouton E, Béguet-Crespel F, Lapaque N, Doré J, Arulampalam V, Blottière HM 
In:
Scientific Reports (2017) 7: doi: 10.1038/srep43199 
BUB1 mediation of caspase-independent mitotic death determines cell fate
Categories:
Transfection 
Authors:
Niikura Y, Dixit A, Scott R, Perkins G, Kitagawa K 
In:
J Cell Biol (2007) 178(2): 283-96 
Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer
Categories:
Transfection 
Authors:
Vignjevic D, Schoumacher M, Gavert N, Janssen KP, Jih G, Laé M, Louvard D, Ben-Ze'ev A, Robine S 
In:
Cancer Res (2007) 67(14): 6844-53 
Full-length, glycosylated NSP4 is localized to plasma membrane caveolae by a novel raft isolation technique
Categories:
Transfection 
Authors:
Storey SM, Gibbons TF, Williams CV, Parr RD, Schroeder F, Ball JM 
In:
J Virol (2007) 81(11): 5472-83 
TRAIL induces receptor-interacting protein 1-dependent and caspase-dependent necrosis-like cell death under acidic extracellular conditions
Categories:
Transfection 
Authors:
Meurette O, Rebillard A, Huc L, Le Moigne G, Merino D, Micheau O, Lagadic-Gossmann D, Dimanche-Boitrel MT 
In:
Cancer Res (2007) 67(1): 218-26 
Evi1 is a survival factor which conveys resistance to both TGFbeta- and taxol-mediated cell death via PI3K/AKT
Categories:
Transfection 
Authors:
Liu Y, Chen L, Ko TC, Fields AP, Thompson EA 
In:
Oncogene (2006) 25(25): 3565-75 
Silencing of SH-PTP2 defines a crucial role in the inactivation of epidermal growth factor receptor by 5-aminosalicylic acid in colon cancer cells
Categories:
Transfection 
Authors:
Monteleone G, Franchi L, Fina D, Caruso R, Vavassori P, Monteleone I, Calabrese E, Naccari GC, Bellinvia S, Testi R and Pallone F 
In:
Cell Death Differ (2006) 13(2): 202-211 
Integrin alpha5/beta1 expression mediates HER-2 down-regulation in colon cancer cells
Categories:
Transfection 
Authors:
Kuwada SK, Kuang J and Li X 
In:
J Biol Chem (2005) 280(19): 19027-19035 
In vitro and ex vivo activation of the TLR5 signaling pathway in intestinal epithelial cells by a commensal Escherichia coli strain
Categories:
Transfection 
Authors:
Bambou JC, Giraud A, Menard S, Begue B, Rakotobe S, Heyman M, Taddei F, Cerf-Bensussan N and Gaboriau-Routhiau V 
In:
J Biol Chem (2004) 279(41): 42984-42992 
+ Show All

Privacy | Legal | About Lonza

© Lonza. All rights reserved.