Keratinocyte, human

primary human keratinocyte

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Our first media specialised for growing keratinocytes.
Now superceded by KGM-Gold

The original medium for growth of keratinocytes but without Calcium.

KGM-CD Chemically Defined Keratinocyte Growth Medium was designed as a serum-free, non-animal origin, chemically defined cell culture medium to support the isolation, growth and proliferation of primary human keratinocytes in culture. It is the only medium of its kind on the market today and can be used for a variety of applications associated with skin research, wound healing and dermal disorders.

Note: Coating plates with fibronectin or other non-animal derived matrix material is necessary for successful keratinocyte isolations with KGM-CD medium.

Note: Antibiotics are not included with the SingleQuots Kit (CC-4456) and must be purchased separately.

Supports proliferation of primary human keratinocytes in culture.
Drives faster proliferation than the original KGM.
Now superceded by KGM-Gold

A serum-free, calcium free keratinocyte growth medium

Optimized for Normal Human Epidermal Keratinocytes in a serum-free environment.
Provides a nutrient rich medium that provides normal physiological growth characteristics and population doubling times.
KGM-Gold supports low, moderate, and high density keratinocyte cultures better than KGM or KGM-2, without nutrient depletion.

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (11)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Categories:
Transfection 
Authors:
Mulder KW, Wang X, Escriu C, Ito Y, Schwarz RF, Gillis J, Sirokmány G, Donati G, Uribe-Lewis S, Pavlidis P, Murrell A, Markowetz F, Watt FM. 
In:
Nat Cell Biol (2012) 14(7): 753-63 
Categories:
Transfection 
Authors:
Allen D, Winters E, Kenna PF, Humphries P, Jane Farrar G 
In:
J Dermatol Sci (2008) 49(3): 217-25 
Categories:
Transfection 
Authors:
Borrelli S, Testoni B, Callari M, Alotto D, Castagnoli C, Romano RA, Sinha S, Vigano AM, Mantovani R 
In:
BMC Mol Biol (2007) 8(1): 85 
Categories:
Transfection 
Authors:
Zhang JY, Adams AE, Ridky TW, Tao S, Khavari PA 
In:
Cancer Res (2007) 67(8): 3827-34 
Categories:
Transfection 
Authors:
Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA 
In:
Genes Dev (2006) 20(22): 3185-97 
Categories:
Transfection 
Authors:
Xu Y, Shao Y, Voorhees JJ, Fisher GJ 
In:
J Biol Chem (2006) 281(37): 27389-97 
Categories:
Transfection 
Authors:
Xu Y, Tan LJ, Grachtchouk V, Voorhees JJ and Fisher GJ 
In:
J Biol Chem (2005) 280(52): 42694-42700 
Categories:
Transfection 
Authors:
Harada K, Truong AB, Cai T and Khavari PA 
In:
Mol Cell Biol (2005) 25(24): 11122-11130 
Categories:
Transfection 
Authors:
Doi H, Shibata MA, Kiyokane K and Otsuki Y 
In:
J Dermatol Sci (2003) 33: 7-16 
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