PBMC, human

Primary cells isolated from peripheral blood.

Cell Type:
PBMC
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T cell, human U-014 1e6 50% Plasmid (general) µg 100 µl I/II/2b
T cell, human U-017 5e5 50% 50% Plasmid (general) µg 100 µl I/II/2b

Citations (52)

Categories:
Transfection 
Authors:
Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME,  Ashworth A, Marson A.  
In:
Cell (2018) 175(7): 1985-1971 
Categories:
Primary Cells and Media, Transfection 
Authors:
Hamada M1, Nishio N2, Okuno Y3, Suzuki S3, Kawashima N1, Muramatsu H1, Tsubota S4, Wilson MH5, Morita D6, Kataoka S1, Ichikawa D1, Murakami N1, Taniguchi R1, Suzuki K1, Kojima D1, Sekiya Y1, Nishikawa E1, Narita A1, Hama A1, Kojima S1, Nakazawa Y6, Takahashi Y7. 
In:
EBioMedicine (2018) 2352:  
Categories:
Transfection 
Authors:
Hu B, Zou Y, Zhang L, Tang J, Niedermann G, Firat E, Huang X, Zhu X.  
In:
Hum Gene Ther (2018) :  
Categories:
Transfection 
Authors:
Nakazawa Y, Matsuda K, Kurata T, Sueki A, Tanaka M, Sakashita K, Imai C, Wilson MH, Koike K 
In:
J Hematol Oncol (2016) 9 (1): 1-11 
Categories:
Transfection 
Authors:
Xin Huang, Haein Park, Joseph Greene, James Pao, Erin Mulvey, Sophia X. Zhou, Catherine M. Albert, Fred Moy, Deepali Sachdev, Douglas Yee, Christoph Rader, Carl V. Hamby, David M. Loeb, Mitchell S. Cairo, Xianzheng Zhou 
In:
PLoS ONE (2015) 10(7): e0133152 
Categories:
Transfection 
Authors:
Deniger DC, Yu J, Huls MH, Figliola MJ, Mi T, Maiti SN, Widhopf GF 2nd, Hurton LV, Thokala R, Singh H3, Olivares S, Champlin RE, Wierda WG, Kipps TJ, Cooper LJ. 
In:
PLoS Genet (2015) 10(6): e0128151 
Categories:
Primary Cells and Media 
Authors:
Tanimoto A, Ogawa Y, Oki C, Kimoto Y, Nozawa K, Amano W, Noji S, Shiozaki M, Matsuo A, Shinozaki Y, Matsushita M 
In:
Other (2015) 64(1): 41-51 
Categories:
Primary Cells and Media 
Authors:
Mandl M, Schmitz S, Weber C, Hristov M 
In:
PLoS ONE (2014) 9(11): ePub 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Marie Klinge Brimnes, Bjarke Endel Hansen, Leif Kofoed Nielsen, Morten Hanefeld Dziegiel, Claus Henrik Nielsen 
In:
PLoS ONE (2014) 17(9): e113388 
Categories:
Transfection 
Authors:
Chen H1, Fu T, Suh WK, Tsavachidou D, Wen S, Gao J, Ng Tang D, He Q, Sun J, Sharma P. 
In:
Cancer Immunol Immunother (2014) 2(2): 167-76. 
Categories:
Transfection 
Authors:
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In:
PLoS ONE (2013) 8(5): 1-10 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Poole E, Walther A, Raven K, Benedict CA, Mason GM, Sinclair J. 
In:
J Virol (2013) 87(8): 4261-71 
Categories:
Transfection 
Authors:
Torikai H, Reik A, Liu PQ, Zhou Y, Zhang L, Maiti S, Huls H, Miller JC, Kebriaei P, Rabinovitch B, Lee DA, Champlin RE, Bonini C, Naldini L, Rebar EJ, Gregory PD, Holmes MC, Cooper LJ. 
In:
Blood (2012) 119(24): 5697-5705 
Categories:
 
Authors:
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S. 
In:
Stem Cells (2012) 31(3): 458-466 
Categories:
Transfection 
Authors:
Mack AA, Kroboth S, Rajesh D, Wang WB 
In:
PLoS ONE (2011) 6 (11): e27956 
Categories:
Primary Cells and Media 
Authors:
Deuse T, Seifert M, Phillips N, Fire A, Tyan D, Kay M, Tsao PS, Hua X, Velden J, Eiermann T, Volk HD, Reichenspurner H, Robbins RC, Schrepfer S. 
In:
Circulation (2011) 124(11): ePub 
Categories:
Transfection 
Authors:
Lapinski PE, Oliver JA, Kamen LA, Hughes ED, Saunders TL, King PD 
In:
J Immunol (2008) 181(3): 2019-27 
Categories:
Transfection 
Authors:
Paclik D, Danese S, Berndt U, Wiedenmann B, Dignass A, Sturm A 
In:
PLoS ONE (2008) 3(7): e2629 
Categories:
Transfection 
Authors:
Kumar A, Zloza A, Moon RT, Watts J, Tenorio AR, Al-Harthi L 
In:
J Virol (2008) 82(6): 2813-20 
Categories:
Transfection 
Authors:
Betzi S, Restouin A, Opi S, Arold ST, Parrot I, Guerlesquin F, Morelli X, Collette Y 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19256-61 
Categories:
Transfection 
Authors:
Ishaq M, Degray G, Mou K, Aguilera A, Yang J, Lempicki RA, Hazen A, Natarajan V 
In:
J Immunol (2007) 179(6): 3851-8 
Categories:
Transfection 
Authors:
Srivastava S, Banerjee H, Chaudhry A, Khare A, Sarin A, George A, Bal V, Durdik JM, Rath S 
In:
J Immunol (2007) 179(2): 797-803 
Categories:
Transfection 
Authors:
Fiala M, Liu PT, Espinosa-Jeffrey A, Rosenthal MJ, Bernard G, Ringman JM, Sayre J, Zhang L, Zaghi J, Dejbakhsh S, Chiang B, Hui J, Mahanian M, Baghaee A, Hong P, Cashman J 
In:
Proc Natl Acad Sci USA (2007) 104(31): 12849-54 
Categories:
Transfection 
Authors:
Mummidi S, Adams LM, Vancompernolle SE, Kalkonde M, Camargo JF, Kulkarni H, Bellinger AS, Bonello G, Tagoh H, Ahuja SS, Unutmaz D, Ahuja SK 
In:
J Immunol (2007) 178(9): 5668-81 
Categories:
Transfection 
Authors:
Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, Reynes J, Corbeau P, Jeang KT, Benkirane M 
In:
Science (2007) 315(5818): 1579-82 
Categories:
Transfection 
Authors:
Minguet S, Swamy M, Alarcon B, Luescher IF, Schamel WW 
In:
Immunity (2007) 26(1): 43-54 
Categories:
Transfection 
Authors:
Hayashi K, Altman A 
In:
J Immunol (2006) 177(3): 1721-8 
Categories:
Transfection 
Authors:
Anand AR, Ganju RK 
In:
J Biol Chem (2006) 281(18): 12289-99 
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Categories:
Transfection 
Authors:
Yoshida K, Yamamoto K, Kohno T, Hironaka N, Yasui K, Kojima C, Mukae H, Kadota JI, Suzuki S, Honma K, Kohno S and Matsuyama T 
In:
Int Immunol (2005) 17(11): 1463-1471 
Categories:
Transfection 
Authors:
Tenbrock K, Kyttaris VC, Ahlmann M, Ehrchen JM, Tolnay M, Melkonyan H, Mawrin C, Roth J, Sorg C, Juang YT and Tsokos GC 
In:
J Immunol (2005) 175(9): 5975-5980 
Categories:
Transfection 
Authors:
van Baalen CA, Kwa D, Verschuren EJ, Reedijk ML, Boon AC, de Mutsert G, Rimmelzwaan GF, Osterhaus AD and Gruters RA 
In:
J Infect Dis (2005) 192(7): 1183-1190 
Categories:
Transfection 
Authors:
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In:
Proc Natl Acad Sci USA (2005) 102(36): 12861-12866 
Categories:
Transfection 
Authors:
Xu J, Vallejo AN, Jiang Y, Weyand CM and Goronzy JJ 
In:
J Biol Chem (2005) 280(25): 24277-24285 
Categories:
Transfection 
Authors:
Vicente-Manzanares M, Cruz-Adalia A, Martin-Cofreces NB, Cabrero JR, Dosil M, Alvarado-Sanchez B, Bustelo XR, Sanchez-Madrid F 
In:
Blood (2005) 105(8): 3026-3034 
Categories:
Transfection 
Authors:
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In:
Blood (2005) 105(6): 2449-2457 
Categories:
Transfection 
Authors:
Rose JJ, Janvier K, Chandrasekhar S, Sekaly RP, Bonifacino JS and Venkatesan S 
In:
J Biol Chem (2005) 280(9): 7413-7426 
Categories:
Transfection 
Authors:
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In:
Nat Cell Biol (2005) 7(3): 303-310 
Categories:
Transfection 
Authors:
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In:
J Virol (2005) 79(3): 1655-1665 
Categories:
Transfection 
Authors:
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In:
Nat Immunol (2005) 6(1): 80-89 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Mol Ther (2005) 11(1): 120-131 
Categories:
Transfection 
Authors:
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In:
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Categories:
Transfection 
Authors:
Moriuchi M and Moriuchi H 
In:
J Virol (2004) 78(22): 12709-12711 
Categories:
Transfection 
Authors:
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In:
Nat Immunol (2004) 5(10): 1036-1044 
Categories:
Transfection 
Authors:
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In:
Gene Ther (2004) 11(13): 1068-1080 
Categories:
Transfection 
Authors:
Lin B, Kolluri SK, Lin F, Liu W, Han YH, Cao X, Dawson MI, Reed JC and Zhang XK 
In:
Cell (2004) 116(4): 527-540 
Categories:
Transfection 
Authors:
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In:
J Virol (2003) 77(20): 11170-11179 
Categories:
Transfection 
Authors:
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In:
J Virol (2003) 77(19): 10719-10724 
Categories:
Transfection 
Authors:
Aringer M, Hofmann S, Frucht DM, Chen M, Centola M, Morinobu A, Visconti R, Kastner DL, Smolen JS and O'Shea JJ 
In:
J Immunol (2003) 170(12): 6057-6064 
Categories:
Transfection 
Authors:
Moriuchi M and Moriuchi H 
In:
J Biol Chem (2003) 278(15): 13003-13007 
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