Macrophage, human

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Monocyte-derived. Macrophages are immune cells found all over the body. They act as scavengers that engulf dead cells, foreign substances, and other debris.

Cell Type:
Macrophage
Tissue Origin:
blood
Species:
human
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Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 DP-148 1e5 40-45% 52-70% Plasmid (general) 0.4 µg 20 µl 384-well
P3 96-DP-148 1e5 40-44% 52-68% Plasmid (general) 0.4 µg 20 µl 96-well
P3

DP-148

 1e5 40-44% 52-68% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
Macrophage, human Y-010 5e5 44-74% 79-97% Plasmid (general) 2 µg 100 µl I/II/2b
Macrophage, human Y-010 5e5 44-66% 81-93% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (38)

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A. 
In:
J Exp Med (2020) 217(7): e20191692 
Fast-track development of an in vitro 3D lung/immune cell model to study Aspergillus infections.
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Chandorkar P, Posch W, Zaderer V, Blatzer M, Steger M, Ammann CG, Binder U, Hermann M, Hörtnagl P, Lass-Flörl C, Wilflingseder D 
In:
Scientific Reports (2017) 7(1): 10.1038/s41598-017-11271-4 
Mincle Activation and the Syk/Card9 Signaling Axis are Central to the Development of the Autoimmune Disease of the Eye
Categories:
Serum-free and Speciality Media 
Authors:
Ellen J. Lee, Brieanna R. Brown, Emily E. Vance, Paige E. Snow, Phyllis B. Silver, David Heinrichs, Xin Lin, Yoichiro Iwakura, Christine A. Wells, Rachel R. Caspi and Holly L. Rosenzweig 
In:
J Immunol (2016) 196: 7 
Gene Regulatory Network Inference of Immunoresponsive Gene 1 (IRG1) Identifies Interferon Regulatory Factor 1 (IRF1) as Its Transcriptional Regulator in Mammalian Macrophages.
Categories:
Transfection 
Authors:
Tallam A, Perumal TM, Antony PM, Jäger C, Fritz JV, Vallar L, Balling R, Del Sol A, Michelucci A. 
In:
PLoS ONE (2016) 11(2): 1-28 
Human macrophage SCN5A activates an innate immune signaling pathway for antiviral host defense.
Categories:
Primary Cells and Media 
Authors:
Jones A, Kainz D, Khan F, Lee C, Carrithers MD 
In:
J Biol Chem (2014) 289(51): 35326-40 
Advanced Glycation End Products (AGEs) Cause Pro-atherogenic Changes in Cholesterol Transport: A Possible Mechanism for Cardiovascular Risk in Diabetes
Categories:
Primary Cells and Media 
Authors:
Gamez, JD, Voloshyna I, Littlefield MJ, Castro-Magana M, Reiss AB 
In:
Other (2014) S11:005: ePub 
Small molecule screen yields inhibitors of pseudomonas homoserine lactone-induced host responses.
Categories:
 
Authors:
Valentine CD, Zhang H, Phuan PW, Nguyen J, Verkman AS, Haggie PM. 
In:
Cell Microbiol (2014) 16(1): 1-14 
Characterization of coagulation factor synthesis in nine human primary cell types.
Categories:
Primary Cells and Media 
Authors:
Dashty M, Akbarkhanzadeh V, Zeebregts CJ, Spek CA, Sijbrands EJ, Peppelenbosch MP, Rezaee F. 
In:
Scientific Reports (2012) 2(787): ePub 
Ebola virion attachment and entry into human macrophages profoundly effects early cellular gene expression.
Categories:
Primary Cells and Media 
Authors:
Wahl-Jensen V, Kurz S, Feldmann F, Buehler LK, Kindrachuk J, DeFilippis V, da Silva Correia J, Früh K, Kuhn JH, Burton DR, Feldmann H. 
In:
Other (2011) 5(10): ePub 
Increased serum and bone matrix levels of transforming growth factor 1 in patients with GH deficiency in response to GH treatment
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Bioassay  
Authors:
Ueland T, Lekva T, Otterdal K, Dahl TB, Olarescu NC, Jørgensen AP, Fougner KJ, Brixen K, Aukrust P, Bollerslev J 
In:
Eur J Endocrinol (2011) 165(3): 393-400 
Overexpression of STARD3 in human monocyte/macrophages induces an anti-atherogenic lipid phenotype.
Categories:
Primary Cells and Media, Transfection 
Authors:
Borthwick F, Allen AM, Taylor JM, Graham A 
In:
Other (2010) 119(7): 265-72 
Efficient non-viral transfection of THP-1 cells
Categories:
Transfection 
Authors:
Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O, Robenek H, Lorkowski S. 
In:
J Immunol Methods (2009) 344(2): 109-15 
The Role of Phospholipase D (PLD) and Grb2 in Chemotaxis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Knapek KJ 
In:
Other (2008) 0:0: Thesis 
Constitutive activation of PI3-kinase signaling pathway downregulates TLR4-mediated TNF-alpha release in alveolar macrophages from asymptomatic HIV+ persons in vitro
Categories:
Transfection 
Authors:
Tachado SD, Li X, Swan K, Patel N, Koziel H 
In:
J Biol Chem (2008) 283(48): 33191-8 
NF-kappaB-mediated activation of the chemokine CCL22 by the product of the human cytomegalovirus gene UL144 escapes regulation by viral IE86
Categories:
Transfection 
Authors:
Poole E, Atkins E, Nakayama T, Yoshie O, Groves I, Alcami A, Sinclair J 
In:
J Virol (2008) 82(9): 4250-6 
CD63 is not Required for the Production of Infectious Human Immunodeficiency Virus Type 1 in Human Macrophages
Categories:
Transfection 
Authors:
Ruiz-Mateos E, Pelchen-Matthews A, Deneka M, Marsh M 
In:
J Virol (2008) 82(10): 4751-61 
NAIP and Ipaf control Legionella pneumophila replication in human cells
Categories:
Transfection 
Authors:
Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B 
In:
J Immunol (2008) 180(10): 6808-15 
Signaling mechanism of HIV-1 gp120 and virion-induced IL-1beta release in primary human macrophages
Categories:
Transfection 
Authors:
Cheung R, Ravyn V, Wang L, Ptasznik A, Collman RG 
In:
J Immunol (2008) 180(10): 6675-84 
CLEC5A is critical for dengue-virus-induced lethal disease
Categories:
Transfection 
Authors:
Chen ST, Lin YL, Huang MT, Wu MF, Cheng SC, Lei HY, Lee CK, Chiou TW, Wong CH, Hsieh SL 
In:
Nature (2008) 453(7195): 672-6 
Cutting Edge: A Transcriptional Repressor and Corepressor Induced by the STAT3-Regulated Anti-Inflammatory Signaling Pathway
Categories:
Transfection 
Authors:
El Kasmi KC, Smith AM, Williams L, Neale G, Panopolous A, Watowich SS, Häcker H, Foxwell BM, Murray PJ 
In:
J Immunol (2007) 179(11): 7215-7219 
Chronic stimulation of Nod2 mediates tolerance to bacterial products
Categories:
Transfection 
Authors:
Hedl M, Li J, Cho JH, Abraham C 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19440-5 
Wiskott-aldrich syndrome protein is a key regulator of the phagocytic cup formation in macrophages
Categories:
Transfection 
Authors:
Tsuboi S, Meerloo J 
In:
J Biol Chem (2007) 282(47): 34194-203 
Localization of HIV-1 Vpr to the nuclear envelope: Impact on Vpr functions and virus replication in macrophages
Categories:
Transfection 
Authors:
Jacquot G, le Rouzic E, David A, Mazzolini J, Bouchet J, Bouaziz S, Niedergang F, Pancino G, Benichou S 
In:
Retrovirology (2007) 4(1): 84 
PDGF Synergistically Enhances IFN--Induced Expression of CXCL10 in Blood-Derived Macrophages: Implications for HIV Dementia
Categories:
Transfection 
Authors:
Dhillon NK, Peng F, Ransohoff RM, Buch S 
In:
J Immunol (2007) 179(5): 2722-30 
Type I IFN Induction in Response to Listeria monocytogenes in Human Macrophages: Evidence for a Differential Activation of IFN Regulatory Factor 3 (IRF3)
Categories:
Transfection 
Authors:
Reimer T, Schweizer M, Jungi TW 
In:
J Immunol (2007) 179(2): 1166-77 
Disparity in IL-12 Release in Dendritic Cells and Macrophages in Response to Mycobacterium tuberculosis Is Due to Use of Distinct TLRs
Categories:
Transfection 
Authors:
Pompei L, Jang S, Zamlynny B, Ravikumar S, McBride A, Hickman SP, Salgame P 
In:
J Immunol (2007) 178(8): 5192-9 
In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53
Categories:
Transfection 
Authors:
Deneka M, Pelchen-Matthews A, Byland R, Ruiz-Mateos E, Marsh M 
In:
J Cell Biol (2007) 177(2): 329-41 
Requirement for a Complex of Wiskott-Aldrich Syndrome Protein (WASP) with WASP Interacting Protein in Podosome Formation in Macrophages
Categories:
Transfection 
Authors:
Tsuboi S 
In:
J Immunol (2007) 178(5): 2987-95 
FcgammaRIII-Dependent Inhibition of Interferon-gamma Responses Mediates Suppressive Effects of Intravenous Immune Globulin
Categories:
Transfection 
Authors:
Park-Min KH, Serbina NV, Yang W, Ma X, Krystal G, Neel BG, Nutt SL, Hu X, Ivashkiv LB 
In:
Immunity (2007) 26(1): 67-78 
Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria
Categories:
Transfection 
Authors:
Onfelt B, Nedvetzki S, Benninger RK, Purbhoo MA, Sowinski S, Hume AN, Seabra MC, Neil MA, French PM, Davis DM 
In:
J Immunol (2006) 177(12): 8476-83 
Plasma membrane is the site of productive HIV-1 particle assembly
Categories:
Transfection 
Authors:
Jouvenet N, Neil SJ, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD 
In:
PLoS Biol (2006) 4(12): e435 
The Kinesin KIF1C and Microtubule Plus Ends Regulate Podosome Dynamics in Macrophages
Categories:
Transfection 
Authors:
Kopp P, Lammers R, Aepfelbacher M, Woehlke G, Rudel T, Machuy N, Steffen W, Linder S 
In:
Mol Biol Cell (2006) 17(6): 2811-2823 
IFN-gamma Suppresses IL-10 Production and Synergizes with TLR2 by Regulating GSK3 and CREB/AP-1 Proteins
Categories:
Transfection 
Authors:
Hu X, Paik PK, Chen J, Yarilina A, Kockeritz L, Lu TT, Woodgett JR, Ivashkiv LB 
In:
Immunity (2006) 24(5): 563-74 
Novel stabilin-1 interacting chitinase-like protein (SI-CLP) is upregulated in alternatively activated macrophages and secreted via lysosomal pathway
Categories:
Transfection 
Authors:
Kzhyshkowska J, Mamidi S, Gratchev A, Kremmer E, Schmuttermaier C, Krusell L, Haus G, Utikal J, Schledzewski K, Scholtze J and Goerdt S 
In:
Blood (2006) 107(8): 3221-8 
Selective regulation of IL-10 signaling and function by zymosan
Categories:
Transfection 
Authors:
Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB 
In:
J Immunol (2006) 176(8): 4785-92 
The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
A Salmonella protein causes macrophage cell death by inducing autophagy
Categories:
Transfection 
Authors:
Hernandez LD, Pypaert M, Flavell RA and Galan JE 
In:
J Cell Biol (2003) 163(5): 1123-1131 
Recognition of a novel stage of betaherpesvirus latency in human herpesvirus 6
Categories:
Transfection 
Authors:
Kondo K, Sashhara J, Shimada K, Takemoto M, Amo K, Miyagawa H and Yamanishi K 
In:
J Virol (2003) 77(3): 2258-2264 
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