BEAS-2B

bronchial epithelial

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Airway cell media are serum-free media that have been optimized for the proliferation of airway cells.

BEGM is optimized to provide the best growth for Normal Human Bronchial/Tracheal Epithelial Cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T G-016 1e6 30% Plasmid (general) 2 µg 100 µl I/II/2b
T G-016 1e6 80% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (13)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xia YC, Radwan A, Keenan CR, Langenbach SY, Li M, Radojicic D, Londrigan SL, Gualano RC, Stewart AG 
In:
PLoS Pathog (2017) 13(1): doi: 10.1371/journal.ppat.1006138 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Scheraga RG, Thompson C, Tulapurkar ME, Nagarsekar AC, Cowan M, Potla R, Sun J, Cai R, Logun C, Shelhamer J, Todd NW, Singh IS, Luzina IG, Atamas SP, Hasday JD 
In:
Am J Physiol Lung Cell Mol Physiol (2016) 311(5): L941-L955 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xu H, Sun H, Zhang H, Liu J, Fan F, Li Y, Ning X, Sun Y, Dai S, Liu B, Gao M, Fu S, Zhou C 
In:
PLoS ONE (2015) 10(5): e0124033 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Itoigawa Y, Harada N, Harada S, Katsura Y, Makino F, Ito J, Nurwidya F, Kato M, Takahashi F, Atsuta R, Takahashi K 
In:
Respir Res (2015) 16: 48 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
El Najjar F, Lampe L, Baker ML, Wang LF, Dutch RE 
In:
PLoS ONE (2015) 10(2): e0115736 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Furukawa M, Wheeler S, Clark AM, Wells A 
In:
PLoS ONE (2015) 10 (1): e0118060 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rosa I. Rodríguez-Cotto, Mario G. Ortiz-Martínez, Evasomary Rivera-Ramírez, Vinicius L. Mateus d, Beatriz S. Amaral d, Braulio D. Jimenez-Velez, Adriana Gioda 
In:
Environ Pollut (2014) 194: 112-20 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shoaib Majeed, Stefan Frentzel, Sandra Wagner, Diana Kuehn, Patrice Leroy, Philippe A Guy, Arno Knorr, Julia Hoeng and Manuel C Peitsch 
In:
Chemistry Central (2014) 8(1): 62 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tacon CE, Newton R, Proud D, Leigh R. 
In:
J Immunol (2012) 88(9): 4621-30 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xiaomin Wang, Mirek Mychajlowycz, Christine Lau, Carlos Gutierrez, Jeremy A. Scott, and Chung-Wai Chow 
In:
J Pharmacol Exp Ther (2012) 240(2): 277-85 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
M.H. Hudy, S.L. Traves, S. Wiehler and D. Proud 
In:
Eur Respir J (2010) 35(6): 1256-63 
Categories:
Transfection 
Authors:
Lau C, Wang X, Song L, North M, Wiehler S, Proud D, Chow CW 
In:
J Immunol (2008) 180(2): 870-80 
Categories:
Transfection 
Authors:
Cheng G, Shao Z, Chaudhari BR, Agrawal DK 
In:
Am J Physiol Lung Cell Mol Physiol (2007) 293(5): L1339-47 
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