Calu-3

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adenocarcinoma

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE EO-120 1e6 79-81% 54-56% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE EO-120 2e5 80-82% 52-54% Plasmid (general) 400 ng 20 µl 4D X-Unit
V T-020 1e6 35% Plasmid (general) 2 µg 100 µl I/II/2b
V T-024 1e6 40% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Powell JD, Hutchison JR, Hess BM, Straub TM 
In:
Other (2015) 119(3): 711-23 
PKCdelta acts upstream of SPAK in the activation of NKCC1 by hyperosmomtic stress in airway epithelial cells
Categories:
Transfection 
Authors:
Smith L, Smallwood N, Altman A, Liedtke CM 
In:
J Biol Chem (2008) 283(32): 22147-56 
Role of the scaffold protein RACK1 in apical expression of the cystic fibrosis transmembrane regulator (CFTR)
Categories:
Transfection 
Authors:
Auerbach M, Liedtke CM 
In:
Am J Physiol Cell Physiol (2007) 293(1): C294-304 

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