INS-1

pancreatic beta-cells

Cell Type:
Pancreas
Tissue Origin:
pancreas
Species:
rat
Research Area:
Gastroenterology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-020 2e6 80% Plasmid (general) 2 µg 100 µl I/II/2b
V T-027 2e6 60% Plasmid (general) 2 µg 100 µl I/II/2b
T T-020 1e6 90% 75% Plasmid (general) µg 100 µl I/II/2b
V T-027 1e6 70% 85% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (7)

Categories:
Transfection 
Authors:
Trajkovski M, Mziaut H, Schubert S, Kalaidzidis Y, Altkrueger A, Solimena M 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Lipson KL, Fonseca SG, Ishigaki S, Nguyen LX, Foss E, Bortell R, Rossini AA, Urano F 
In:
Cell Metab (2006) 4(3): 245-54 
Categories:
Transfection 
Authors:
Jensen MV, Joseph JW, Ilkayeva O, Burgess S, Lu D, Ronnebaum SM, Odegaard M, Becker TC, Sherry AD, Newgard CB 
In:
J Biol Chem (2006) 281(31): 22342-22351 
Categories:
Transfection 
Authors:
Knoch KP, Meisterfeld R, Kersting S, Bergert H, Altkruger A, Wegbrod C, Jager M, Saeger HD, Solimena M 
In:
Cell Metab (2006) 3(2): 123-34 
Categories:
Transfection 
Authors:
Fonseca SG, Fukuma M, Lipson KL, Nguyen LX, Allen JR, Oka Y and Urano F 
In:
J Biol Chem (2005) 280(47): 39609-39615 
Categories:
Transfection 
Authors:
Trajkovski M, Mziaut H, Altkruger A, Ouwendijk J, Knoch KP, Muller S and Solimena M 
In:
J Cell Biol (2004) 167(6): 1063-1074 
Categories:
Transfection 
Authors:
Knoch KP, Bergert H, Borgonovo B, Saeger HD, Altkruger A, Verkade P, Solimena M 
In:
Nat Cell Biol (2004) 6(3): 207-14 
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