H4
Export
neuroglioma, epithelial like
Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
human
Research Area:
Neurobiology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SG
CM-113
2e5
79%
79-81%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SG
CM-113
1e6
77-79%
79-83%
Plasmid (general)
2 µg
100 µl
4D X-Unit
V
T-016
1e6
75%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
O-017
1e6
70%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-020
1e6
30%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-027
1e6
80%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
SG
DS-138
2e5
78%
Plasmid (general)
400 ng
20 µl
96-well
SG
FF-120
2e5
72%
Plasmid (general)
400 ng
20 µl
96-well
Citations
Isoflurane-induced caspase-3 activation is dependent on cytosolic calcium and can be attenuated by memantine
Categories:
Transfection
Authors:
Zhang G, Dong Y, Zhang B, Ichinose F, Wu X, Culley DJ, Crosby G, Tanzi RE, Xie Z
In:
J Neurosci (2008) 28(17): 4551-60
Knockdown of ACAT-1 reduces amyloidogenic processing of APP
Categories:
Transfection
Authors:
Huttunen HJ, Greco C, Kovacs DM
In:
FEBS Lett (2007) 581(8): 1688-92
RNA interference silencing of the adaptor molecules ShcC and fe65 differentially affect amyloid precursor protein processing and Abeta generation
Categories:
Transfection
Authors:
Xie Z, Dong Y, Maeda U, Xia W, Tanzi RE
In:
J Biol Chem (2007) 282(7): 4318-4325
Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion
Categories:
Transfection
Authors:
Hiltunen M, Lu A, Thomas AV, Romano DM, Kim M, Jones PB, Xie Z, Kounnas MZ, Wagner SL, Berezovska O, Hyman BT, Tesco G, Bertram L, Tanzi RE
In:
J Biol Chem (2006) 281(43): 32240-32253
Artefactual effects of lipid-based cell transfection reagents on AbetaPP processing and Abeta production
Categories:
Transfection
Authors:
Romano DM, Dong Y, Hiltunen M, Tanzi RE, Xie Z
In:
Amyloid (2006) 13(2): 86-92
RNAi-mediated silencing of X11alpha and X11beta attenuate Abeta levels via differential effects on APP processing
Categories:
Transfection
Authors:
Xie Z, Romano DM and Tanzi RE
In:
J Biol Chem (2005) 280(15): 15413-15421