Colo 205

human colon adenocarcinoma

Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SG DP-113 2e5 57-59% 69-75% Plasmid (general) 1 µg 20 µl 4D X-Unit
SG DP-113 1e6 53% 63-65% Plasmid (general) 5 µg 100 µl 4D X-Unit
T A-024 1e6 68% 70% Plasmid (general) 10 µg 100 µl I/II/2b
T A-013 1e6 55% 80% Plasmid (general) 10 µg 100 µl I/II/2b
V A-024 1e6 40% 70% Plasmid (general) 10 µg 100 µl I/II/2b
V A-013 1e6 30% 80% Plasmid (general) 10 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Corvaisier M, Moreau-Aubry A, Diez E, Bennouna J, Mosnier JF, Scotet E, Bonneville M and Jotereau F 
In:
J Immunol (2005) 175(8): 5481-5488