SMC, aortic (AoSMC), human

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Smooth muscle cells isolated from human aorta.

Cell Type:
Smooth muscle cell
Tissue Origin:
aortic
Species:
human
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Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Smooth Muscle Growth Medium-2

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1

EH-106

 1e5 74% 92% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1

EH-106

 1e5 74% 92% Plasmid (general) 0.2 µg 20 µl 96-well
AoSMC, human U-025 1e6 57-79% 90-96% Plasmid (general) 5 µg 100 µl I/II/2b
Smooth muscle, basic P-024 2e6 75% 69% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (37)

Nicotine Stimulates Nerve Growth Factor in Lung Fibroblasts through an NF?B-Dependent Mechanism
Categories:
Primary Cells and Media 
Authors:
Wongtrakool C1, Grooms K2, Bijli KM1, Crothers K3, Fitzpatrick AM4, Hart CM1 
In:
PLoS ONE (2014) 9(10): e109602 
Hox genes are involved in vascular wall-resident multipotent stem cell differentiation into smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Klein D, Benchellal M, Kleff V, Jakob HG, Ergün S 
In:
Scientific Reports (2013) 3: 2178 
Circulating angiopoietin-2 is a marker for early cardiovascular disease in children on chronic dialysis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shroff RC, Price KL, Kolatsi-Joannou M, Todd AF, Wells D, Deanfield J, Johnson RJ, Rees L, Woolf AS, Long DA 
In:
PLoS ONE (2013) 8(2): e56273 
Thrombospondin-1 regulates blood flow via CD47 receptor-mediated activation of NADPH oxidase 1.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Csányi G, Yao M, Rodríguez AI, Al Ghouleh I, Sharifi-Sanjani M, Frazziano G, Huang X, Kelley EE, Isenberg JS, Pagano PJ. 
In:
Arterioscler Thromb Vasc Biol (2012) 32(12): 2966-73 
Reduced elastogenesis: a clue to the arteriosclerosis and emphysematous changes in Schimke immuno-osseous dysplasia?
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morimoto M, Yu Z, Stenzel P, Clewing JM, Najafian B, Mayfield C, Hendson G, Weinkauf JG, Gormley AK, Parham DM, Ponniah U, André JL, Asakura Y, Basiratnia M, Bogdanovic R, Bokenkamp A, Bonneau D, Buck A, Charrow J, Cochat P, Cordeiro I, Deschenes G, Fenkçi MS, Frange P, Fründ S, Fryssira H, Guillen-Navarro E, Keller K, Kirmani S, Kobelka C, Lamfers P, Levtchenko E, Lewis DB, Massella L, McLeod DR, Milford DV, Nobili F, Saraiva JM, Semerci CN, Shoemaker L, Stajic N, Stein A, Taha D, Wand D, Zonana J, Lücke T, Boerkoel CF. 
In:
Orphanet Journal of Rare Diseases (2012) 7: 70 
Differential susceptibility of human primary aortic and coronary artery vascular cells to RNA interference.
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zouaoui Boudjeltia K, Daher J, Van Antwerpen P, Moguilevsky N, Delree P, Ducobu J, Raes M, Badran B, Vanhaeverbeek M, Brohee D, Remacle C, Vanhamme L. 
In:
PLoS ONE (2012) 7: 0038810 
Critical role of the a1-Na+, K +-ATPase subunit in insensitivity of rodent cells to cytotoxic action of ouabain.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akimova OA, Tverskoi AM, Smolyaninova LV, Mongin AA, Lopina OD, La J, Dulin NO, Orlov SN 
In:
Apoptosis (2012) ePub: e 
Reciprocal regulation of syndecan-2 and Notch signaling in vascular smooth muscle cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhao N, Liu H, Lilly B 
In:
J Biol Chem (2012) 287(20): 16111-20 
TRAIL promotes caspase-dependent pro-inflammatory responses via PKCd activation by vascular smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Song S, Choi K, Ryu SW, Kang SW, Choi C 
In:
Cell Death Differ (2011) 2: e223 
Plasma Kallikrein Promotes Epidermal Growth Factor Receptor Transactivation and Signaling in Vascular Smooth Muscle through Direct Activation of Protease-activated Receptors*
Categories:
Primary Cells and Media 
Authors:
Abdallah RT, Keum JS, El-Shewy HM, Lee MH, Wang B, Gooz M, Luttrell DK, Luttrell LM, Jaffa AA. 
In:
J Biol Chem (2011) 286(26): 23620 
TNF-a response of vascular endothelial and vascular smooth muscle cells involve differential utilization of ASK1 kinase and p73
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rastogi S, Rizwani W, Joshi B, Kunigal S, Chellappan SP 
In:
Cell Death Differ (2011) 19(2): 274-83 
Systemic activation of the transient receptor potential vanilloid subtype 4 channel causes endothelial failure and circulatory collapse: Part 2
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Willette RN, Bao W, Nerurkar S, Yue TL, Doe CP, Stankus G, Turner GH, Ju H, Thomas H, Fishman CE, Sulpizio A, Behm DJ, Hoffman S, Lin Z, Lozinskaya I, Casillas LN, Lin M, Trout RE, Votta BJ, Thorneloe K, Lashinger ES, Figueroa DJ, Marquis R, Xu X. 
In:
J Pharmacol Exp Ther (2011) 338(1): 408-9 
Concentration independent modulation of local micromechanics in a fibrin gel
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kotlarchyk MA, Shreim SG, Alvarez-Elizondo MB, Estrada LC, Singh R, Valdevit L, Kniazeva E, Gratton E, Putnam AJ, Botvinick EL 
In:
PLoS ONE (2011) 6(5): 1-12 
MicroRNA-26a is a novel regulator of vascular smooth muscle cell function
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Leeper NJ, Raiesdana A, Kojima Y, Chun HJ, Azuma J, Maegdefessel L, Kundu RK, Quertermous T, Tsao PS, Spin JM 
In:
J Cell Physiol (2011) 226(4): 1035-43 
High throughput RNAi assay optimization using adherent cell cytometry
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nabzdyk CS, Chun M, Pradhan L, Logerfo FW 
In:
J Transl Med (2011) 9(48): 1-9 
Hyaluronan Synthesis Is Inhibited by Adenosine Monophosphate-activated Protein Kinase through the Regulation of HAS2 Activity in Human Aortic Smooth Muscle Cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vigetti D, Clerici M, Deleonibus S, Karousou E, Viola M, Moretto P, Heldin P, Hascall VC, De Luca G, Passi A 
In:
J Biol Chem (2011) 286(10): 7917-24 
BMP promotes motility and represses growth of smooth muscle cells by activation of tandem Wnt pathways
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Perez VA, Ali Z, Alastalo TP, Ikeno F, Sawada H, Lai YJ, Kleisli T, Spiekerkoetter E, Qu X, Rubinos LH, Ashley E, Amieva M, Dedhar S, Rabinovitch M 
In:
J Cell Biol (2011) 192(1): 171-88 
Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mendonça MC, Koles N, Doi SQ, Sellitti DF. 
In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Smooth Muscle Cell–Specific Insulin-Like Growth Factor-1 Overexpression in Apoe/ Mice Does Not Alter Atherosclerotic Plaque Burden but Increases Features of Plaque Stability
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shai SY, Sukhanov S, Higashi Y, Vaughn C, Kelly J, Delafontaine P 
In:
Arterioscler Thromb Vasc Biol (2010) 30(10): 1916-24 
High-Phosphate-Induced Calcification Is Related to SM22a Promoter Methylation in Vascular Smooth Muscle Cells
Categories:
Primary Cells and Media 
Authors:
Montes de Oca A, Madueño JA, Martinez-Moreno JM, Guerrero F, Muñoz-Castañeda J, Rodriguez-Ortiz ME, Mendoza FJ, Almaden Y, Lopez I, Rodriguez M, Aguilera-Tejero E 
In:
Other (2010) 25(9): 1996-2005 
Effects of a novel cystine-based glutathione precursor on oxidative stress in vascular smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sinha-Hikim I, Shen R, Paul Lee WN, Crum A, Vaziri ND, Norris KC. 
In:
Am J Physiol Cell Physiol (2010) 299(3): C638-42 
Adipose tissue progenitor cells directly interact with endothelial cells to induce vascular network formation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merfeld-Clauss S, Gollahalli N, March KL, Traktuev DO. 
In:
Tissue Eng (2010) 16(9): 2953-66 
Mechanism of Improved Cardiac Function After Bone Marrow Mononuclear Cell Therapy
Categories:
Primary Cells and Media 
Authors:
Yoon CH, Koyanagi M, Iekushi K, Seeger F, Urbich C, Zeiher AM, Dimmeler S. 
In:
Circulation (2010) 121(18): 2001-11 
Effect of endothelium mimicking self-assembled nanomatrices on cell adhesion and spreading of human endothelial cells and smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Andukuri A, Minor WP, Kushwaha M, Anderson JM, Jun HW 
In:
Other (2010) 6(2): 289-97 
A nitric oxide releasing, self assembled peptide amphiphile matrix that mimics native endothelium for coating implantable cardiovascular devices
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kushwaha M, Anderson JM, Bosworth CA, Andukuri A, Minor WP, Lancaster JR Jr, Anderson PG, Brott BC, Jun HW 
In:
Biomaterials (2010) 31(7): 1502-8 
Role of homocysteine in aortic calcification and osteogenic cell differentiation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Van Campenhout A, Moran CS, Parr A, Clancy P, Rush C, Jakubowski H, Golledge J 
In:
Other (2009) 202(2): 557-566 
The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension
Categories:
Transfection 
Authors:
Nieves-Cintron M, Amberg GC, Navedo MF, Molkentin JD, Santana LF 
In:
Proc Natl Acad Sci USA (2008) 105(40): 15623-8 
A thermoresponsive, microtextured substrate for cell sheet engineering with defined structural organization
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Isenberg BC, Tsuda Y, Williams C, Shimizu T, Yamato M, Okano T, Wong JY 
In:
Biomaterials (2008) 29(17): 2565-72 
Glycosylation mediates upregulation of a potent antiangiogenic and proatherogenic protein, Thrombospondin-1, by glucose in vascular smooth muscle cells
Categories:
Transfection 
Authors:
Raman P, Krukovets I, Marinic TE, Bornstein P, Stenina OI 
In:
J Biol Chem (2007) 282(8): 5704-14 
Novel Effect of Oxidized Low-Density Lipoprotein. Cellular ATP Depletion via Downregulation of Glyceraldehyde-3-Phosphate Dehydrogenase
Categories:
Transfection 
Authors:
Sukhanov S, Higashi Y, Shai SY, Itabe H, Ono K, Parthasarathy S, Delafontaine P 
In:
Circ Res (2006) 99(2): 191-200 
Matrix metalloproteinase 2 and tissue inhibitors of metalloproteinases regulate human aortic smooth muscle cell migration during in vitro aging
Categories:
Transfection 
Authors:
Vigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Pallotti F, De Luca G, Passi A 
In:
FASEB J (2006) 20(8): 1118-1130 
Key role of SRC kinase in S100B-induced activation of the receptor for advanced glycation end products in vascular smooth muscle cells
Categories:
Transfection 
Authors:
Reddy MA, Li SL, Sahar S, Kim YS, Xu ZG, Lanting L, Natarajan R 
In:
J Biol Chem (2006) 281(19): 13685-93 
Molecular cloning and characterization of UDP-glucose dehydrogenase from the amphibian xenopus laevis and its involvement in hyaluronan synthesis
Categories:
Transfection 
Authors:
Vigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F, Nardi I, Hascall VC, De Luca G and Passi A 
In:
J Biol Chem (2006) 281(12): 8254-8263 
Prostaglandin I2 Production and cAMP Accumulation in Response to Acidic Extracellular pH through OGR1 in Human Aortic Smooth Muscle Cells
Categories:
Transfection 
Authors:
Tomura H, Wang JQ, Komachi M, Damirin A, Mogi C, Tobo M, Kon J, Misawa N, Sato K and Okajima F 
In:
J Biol Chem (2005) 280(41): 34458-34464 
Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
Genet Vaccines Ther (2005) 3(1): 2 
The aorta and heart differentially express RGS (regulators of G-protein signalling) proteins that selectively regulate sphingosine 1-phosphate, angiotensin II and endothelin-1 signalling
Categories:
Transfection 
Authors:
Cho H, Harrison K, Schwartz O, Kehrl JH 
In:
Biochem J (2003) 371(Pt 3): 973-80 
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