Embryonic stem cell (ES), mouse

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Stem cells derived from mouse blastocyst.

Cell Type:
Embryonic SC
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Stem Cells
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-CG-104 5e4 85-95% 58-88% Plasmid (general) 0.4 µg 20 µl 96-well
ES cell, mouse A-013 5e6 80% Plasmid (general) 9 µg 100 µl I/II/2b
P3

CG-104

 5e4 85-95% 58-88% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (31)

Generation of locus-specific degradable tag knock-ins in mouse and human cell lines
Categories:
Transfection 
Authors:
Damhofer H, Radzisheuskaya A, Helin K. 
In:
STAR Protoc (2021) 2(2): 100575 
An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein.
Categories:
Primary Cells and Media, Transfection 
Authors:
Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M, Blin C, Finch R, Bressan RB, Morrison G, Jacobi AM, Behlke MA, von Kriegsheim A, Tomlinson S, Krijgsveld J, Pollard SM. 
In:
eLife (2018) 11;7: 1-29 
CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.
Categories:
Transfection 
Authors:
Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan, Shanbo Cao, Zhou Songyang mail, Junjiu Huang 
In:
PLoS ONE (2015) 10(11): e0143148 
Optimization of genome engineering approaches with the CRISPR/Cas9 system
Categories:
Transfection 
Authors:
Li K1, Wang G1, Andersen T2, Zhou P1, Pu WT3. 
In:
PLoS ONE (2014) 9(8): 1-10 
Isolation and Characterization of Alveolar Epithelial Type II Cells Derived from Mouse Embryonic Stem Cells
Categories:
 
Authors:
Sun H, Quan Y, Yan Q, Peng X, Mao Z, Wetsel RA, Wang D. 
In:
Tissue Eng (2014) 20(6): 464-72 
Targeted genome modification in mice using zinc-finger nucleases. Genetics
Categories:
Transfection 
Authors:
Carbery ID, Ji D, Harrington A, Brown V, Weinstein EJ, Liaw L, Cui X 
In:
Genome Res (2010) 186(2): 451-9 
G Protein–Coupled Receptor APJ and Its Ligand Apelin Act Downstream of Cripto to Specify Embryonic Stem Cells Toward the Cardiac Lineage Through Extracellular Signal-Regulated Kinase/p70S6 Kinase Signaling Pathway
Categories:
Transfection 
Authors:
D'Aniello C, Lonardo E, Iaconis S, Guardiola O, Liguoro AM, Liguori GL, Autiero M, Carmeliet P, Minchiotti G 
In:
Circ Res (2009) 105(3): 211-3 
Splicing of HDAC7 modulates the SRF-myocardin complex during stem-cell differentiation towards smooth muscle cells.
Categories:
Transfection 
Authors:
Margariti A, Xiao Q, Zampetaki A, Zhang Z, Li H, Martin D, Hu Y, Zeng L, Xu Q 
In:
J Cell Sci (2009) 122(Pt 4): 460-70 
Different polycomb group CBX family proteins associate with distinct regions of chromatin using nonhomologous protein sequences
Categories:
Transfection 
Authors:
Vincenz C, Kerppola TK 
In:
Proc Natl Acad Sci USA (2008) 105(43): 16572-7 
B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells
Categories:
Transfection 
Authors:
Tarasov KV, Tarasova YS, Tam WL, Riordon DR, Elliott ST, Kania G, Li J, Yamanaka S, Crider DG, Testa G, Li RA, Lim B, Stewart CL, Liu Y, Van Eyk JE, Wersto RP, Wobus AM, Boheler KR 
In:
PLoS ONE (2008) 3(6): e2478 
REST maintains self-renewal and pluripotency of embryonic stem cells
Categories:
Transfection 
Authors:
Singh SK, Kagalwala MN, Parker-Thornburg J, Adams H, Majumder S 
In:
Nature (2008) 453(7192): 223-7 
Tlx3 exerts context-dependent transcriptional regulation and promotes neuronal differentiation from embryonic stem cells
Categories:
Transfection 
Authors:
Kondo T, Sheets PL, Zopf DA, Aloor HL, Cummins TR, Chan RJ, Hashino E 
In:
Proc Natl Acad Sci USA (2008) 105(15): 5780-5 
MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells
Categories:
Transfection 
Authors:
Sinkkonen L, Hugenschmidt T, Berninger P, Gaidatzis D, Mohn F, Artus-Revel CG, Zavolan M, Svoboda P, Filipowicz W 
In:
Nat Struct Mol Biol (2008) 15(3): 259-67 
Old World Arenavirus Infection Interferes with the Expression of Functional alpha-Dystroglycan in the Host Cell
Categories:
Transfection 
Authors:
Rojek JM, Campbell KP, Oldstone MB, Kunz S 
In:
Mol Biol Cell (2007) 18(11): 4493-507 
E-cadherin inhibits cell surface localization of the pro-migratory 5T4 oncofetal antigen in mouse embryonic stem cells
Categories:
Transfection 
Authors:
Spencer HL, Eastham AM, Merry CL, Southgate TD, Perez-Campo F, Soncin F, Ritson S, Kemler R, Stern PL, Ward CM 
In:
Mol Biol Cell (2007) 18(8): 2838-51 
New cell lines from mouse epiblast share defining features with human embryonic stem cells
Categories:
Transfection 
Authors:
Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD 
In:
Nature (2007) 448(7150): 196-9 
HIF-1alpha inhibits self-renewal of mouse embryonic stem cells in vitro via negative regulation of the LIF-Stat3 pathway
Categories:
Transfection 
Authors:
Jeong CH, Lee HJ, Cha JH, Kim JH, Kim KR, Kim JH, Yoon DK, Kim KW 
In:
J Biol Chem (2007) 282(18): 13672-9 
Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines
Categories:
Transfection 
Authors:
Soto-Gutiérrez A, Navarro-Alvarez N, Zhao D, Rivas-Carrillo JD, Lebkowski J, Tanaka N, Fox IJ, Kobayashi N 
In:
Nat Protocols (2007) 2(2): 347-356 
Expression of the Wnt inhibitor Dickkopf-1 is required for the induction of neural markers in mouse embryonic stem cells differentiating in response to retinoic acid
Categories:
Transfection 
Authors:
Verani R, Cappuccio I, Spinsanti P, Gradini R, Caruso A, Magnotti MC, Motolese M, Nicoletti F, Melchiorri D 
In:
J Neurochem (2007) 100(1): 242-50 
Pulmonary adenocarcinoma-targeted gene therapy by a cancer- and tissue-specific promoter system
Categories:
Transfection 
Authors:
Fukazawa T, Maeda Y, Durbin ML, Nakai T, Matsuoka J, Tanaka H, Naomoto Y, Tanaka N 
In:
Mol Cancer Ther (2007) 6(1): 244-52 
Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switch
Categories:
Transfection 
Authors:
Donohoe ME, Zhang LF, Xu N, Shi Y, Lee JT 
In:
Mol Cell (2007) 25(1): 43-56 
A time- and dose-dependent STAT1 expression system
Categories:
Transfection 
Authors:
Leitner NR, Strobl B, Bokor M, Painz R, Kolbe T, Rulicke T, Muller M, Karaghiosoff M 
In:
BMC Biotechnol (2006) 6: 48 
Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes
Categories:
Transfection 
Authors:
Soto-Gutierrez A, Kobayashi N, Rivas-Carrillo JD, Navarro-Alvarez N, Zhao D, Okitsu T, Noguchi H, Basma H, Tabata Y, Chen Y, Tanaka K, Narushima M, Miki A, Ueda T, Jun HS, Yoon JW, Lebkowski J, Tanaka N, Fox IJ 
In:
Nat Biotechnol (2006) 24(11): 1412-1419 
Self-renewal of embryonic stem cells by a small molecule
Categories:
Transfection 
Authors:
Chen S, Do JT, Zhang Q, Yao S, Yan F, Peters EC, Schöler HR, Schultz PG, Ding S 
In:
Proc Natl Acad Sci USA (2006) 103(46): 17266-71 
Endogenously activated mGlu5 metabotropic glutamate receptors sustain the increase in c-Myc expression induced by leukaemia inhibitory factor in cultured mouse embryonic stem cells
Categories:
Transfection 
Authors:
Spinsanti P, De Vita T, Di Castro S, Storto M, Formisano P, Nicoletti F, Melchiorri D 
In:
J Neurochem (2006) 99(1): 299-307 
Identification of intrinsic determinants of midbrain dopamine neurons
Categories:
Transfection 
Authors:
Andersson E, Tryggvason U, Deng Q, Friling S, Alekseenko Z, Robert B, Perlmann T, Ericson J 
In:
Cell (2006) 124(2): 393-405 
Gene delivery to embryonic stem cells
Categories:
Transfection 
Authors:
Kobayashi N, Rivas-Carrillo JD, Soto-Gutierrez A, Fukazawa T, Chen Y, Navarro-Alvarez N, Tanaka N 
In:
Birth Defects Res C Embryo Today (2005) 75(1): 10-8 
Initial observations on the effect of medium composition on the differentiation of murine embryonic stem cells to alveolar type II cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rippon HJ, Ali NN, Polak JM, Bishop AE. 
In:
Cloning and Stem Cells (2004) 6(2): 49-56 
Defects in Cell Adhesion and the Visceral Endoderm following Ablation of Nonmuscle Myosin Heavy Chain II-A in Mice
Categories:
Transfection 
Authors:
Conti MA, Even-Ram S, Liu C, Yamada KM and Adelstein RS 
In:
J Biol Chem (2004) 279(40): 41263-41266 
Efficient gene transfer into murine embryonic stem cells by nucleofection
Categories:
Transfection 
Authors:
Lorenz P, Harnack U and Morgenstern R 
In:
Biotechnol Lett (2004) 26(20): 1589-1592 
Efficient transfection of embryonic and adult stem cells
Categories:
Transfection 
Authors:
Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS and Verfaillie CM 
In:
Stem Cells (2004) 22(4): 531-543 
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