Melanocyte, (NHEM-neo), human neonatal

Epidermal pigment cells of neural crest origin. Melanocytes contribute pigment (melanin) in the form of membrane-bound melanosomes to developing keratinocytes (epidermal skin cells) and hair or feather shafts.
Samples derived from human neonatal foreskins.

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

MGM-4 has been optimized for the growth and proliferation of normal human primary melanocytes in culture. This medium system has been shown to deliver superior results as compared to other existing commercial media systems. Melanocytes in culture maintain >90% functionality based on the conversion of L-dopa to dopa-melanin. The melanocyte media system also allows for normal morphology and proliferative capacity after recovery from cryopreservation and throughout serial passaging.

Adult melanocytes also require the addition of Et-3 (CC-4510), sold separately

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Melanocyte, human U-024 5e5 63-77% 55-60% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (10)

Categories:
Primary Cells and Media 
Authors:
Bultema JJ, Boyle JA, Malenke PB, Martin FE, Dell\\\'Angelica EC, Cheney RE, Di Pietro SM 
In:
J Biol Chem (2014) 16: 16 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tringali C, Silvestri I, Testa F, Baldassari P, Anastasia L, Mortarini R, Anichini A, López-Requena A, Tettamanti G, Venerando B 
In:
BMC Cancer (2014) 14: 560 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fernández-Barral A, Orgaz JL, Baquero P, Ali Z, Moreno A, Tiana M, Gómez V, Riveiro-Falkenbach E, Cañadas C, Zazo S, Bertolotto C, Davidson I, Rodríguez-Peralto JL, Palmero I, Rojo F, Jensen LD, del Peso L, Jiménez B. 
In:
Neoplasia (2014) 16(6): 529-42 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fernández-Barral A1, Orgaz JL, Gomez V, del Peso L, Calzada MJ, Jiménez B. 
In:
PLoS ONE (2012) 7(3): e32989 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lo Monaco E1, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P. 
In:
Neoplasia (2011) 13(9): 822-30 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Aguzzi MS1, Fortugno P, Giampietri C, Ragone G, Capogrossi MC, Facchiano A. 
In:
Mol Cancer (2010) 9: 84 
Categories:
Transfection 
Authors:
Dumaz N, Hayward R, Martin J, Ogilvie L, Hedley D, Curtin JA, Bastian BC, Springer C, Marais R 
In:
Cancer Res (2006) 66(19): 9483-91 
Categories:
Transfection 
Authors:
Rouzaud F, Costin GE, Yamaguchi Y, Valencia JC, Berens WF, Chen KG, Hoashi T, Bohm M, Abdel-Malek ZA, Hearing VJ 
In:
FASEB J (2006) 20(11): 1927-9 
Categories:
Transfection 
Authors:
Berens W, Van Den Bossche K, Yoon TJ, Westbroek W, Valencia JC, Out CJ, Marie Naeyaert J, Hearing VJ and Lambert J 
In:
Pigment Cell Res (2005) 18(5): 370-381 
Categories:
Transfection 
Authors:
Wellbrock C and Marais R 
In:
J Cell Biol (2005) 170(5): 703-708 
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