CD34+ cell, human

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Hematopoetic stem cells isolated from peripheral blood, bone marrow, cord blood or fetal liver.

CD34 is a known marker for hematopoietic progenitor cells. As these cells mature and differentiate, CD34 expression is lost.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
blood
Species:
human
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Research Area:
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 EO-100-AA 5e4 78-88% 56-68% Plasmid (general) 0.4 µg 20 µl 384-well
P3 EO-100-AA 5e4 65-75% 78-88% Plasmid (general) 0.4 µg 20 µl 384-well
P3 96-EO-100 5e4 78-88% 56-68% Plasmid (general) 0.4 µg 20 µl 96-well
CD34+, human U-008 1e6 60-82% Plasmid (general) µg 100 µl I/II/2b
P3

EO-100

 5e4 62-70% 79-91% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
5e4 Plasmid (general) 20 µl 384-well
5e4 Plasmid (general) 20 µl 384-well

Citations (149)

A simultaneous knockout knockin genome editingstrategy in HSPCs potently inhibits CCR5- andCXCR4-tropic HIV-1 infection
Categories:
Primary Cells and Media, Transfection 
Authors:
Dudek AM, Feist WN, Sasu EJ, Luna SE, Ben-Efraim K, Bak RO, Cepika AM, Porteus MH 
In:
Cell Stem Cell (2024) 31(4): 499-518.e6 
Gene knock-outs in human CD34+hematopoietic stem and progenitor cells andin the human immune system of mice
Categories:
Primary Cells and Media, Transfection 
Authors:
Daniel A Kuppers, Jonathan Linton, Sergio Ortiz Espinosa, Kelly M McKenna, Anthony Rongvaux, Patrick J Paddison  
In:
PLoS ONE (2023) 18(6) : 1-15 
Therapeutic adenine base editing of human hematopoietic stem cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Liao J, Chen S, Hsiao S, Jiang Y, Yang Y, Zhang Y, Wang X, Lai Y, Bauer DE, Wu Y 
In:
Nat Commun. (2023) 14(1): 207 
Molecular dynamics of genome editing with CRISPR-Cas9 and rAAV6 virus in human HSPCs to treat sickle cell disease
Categories:
Transfection 
Authors:
Liwen Xu, Premanjali Lahiri, Jason Skowronski, Neehar Bhatia, Annalisa Lattanzi, and Matthew H. Porteus 
In:
Mol Ther Methods Clin Dev. (2023) 30:  P317-331 
Epitope base editing CD45 in hematopoietic cells enables universal blood cancer immune therapy
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Wellhausen N, O'Connell RP, Lesch S, Engel NW, Rennels AK, Gonzales D, Herbst F, Young RM, Garcia KC, Weiner D, June CH, Gill SI. 
In:
Science (2023) eadi: 1145 
Gene editing of hematopoietic stem cells restores T-cell response in familial hemophagocytic lymphohistiocytosis
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Dettmer-Monaco V, Weißert K, Ammann S, Monaco G, Lei L, Gräßel L, Rhiel M, Rositzka J, Kaufmann MM, Geiger K, Andrieux G, Lao J, Thoulass G, Schell C, Boerries M, Illert AL, Cornu TI, Ehl S, Aichele P, Cathomen T 
In:
J Allergy Clin Immunol (2023) S0091: 6749(23)00989-2 
A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Carusillo A, Haider S, Schäfer R, Rhiel M, Türk D, Chmielewski KO, Klermund J, Mosti L, Andrieux G, Schäfer R, Cornu TI, Cathomen T, Mussolino C 
In:
Nucleic Acids Res (2023) 51(9): 4660-4673 
Adenine base editing efficiently restores the function of Fanconi anemia hematopoietic stem and progenitor cells
Categories:
Transfection 
Authors:
Siegner SM, Ugalde L, Clemens A, Garcia-Garcia L, Bueren JA, Rio P, Karasu ME, Corn JE. 
In:
Nat Commun. (2022) 13(1): 6900 
Ultra-deep sequencing validates safety of CRISPR/Cas9 genome editing in human hematopoietic stem and progenitor cells
Categories:
Transfection 
Authors:
Cromer MK, Barsan VV, Jaeger E, Wang M, Hampton JP, Chen F, Kennedy D, Xiao J, Khrebtukova I, Granat A, Truong T, Porteus MH 
In:
Nat Commun. (2022) 13(1): 4724 
Development of ß-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell disease
Categories:
Transfection 
Authors:
Annalisa Lattanzi , Joab Camarena , Premanjali Lahiri , Helen Segal , Waracharee Srifa , Christopher A Vakulskas 4, Richard L Frock 5, Josefin Kenrick 5, Ciaran Lee 6, Narae Talbott , Jason Skowronski , M Kyle Cromer , Carsten T Charlesworth , Rasmus O Bak , Sruthi Mantri , Gang Bao , David DiGiusto , John Tisdale , J Fraser Wright , Neehar Bhatia , Maria Grazia Roncarolo , Daniel P Dever , Matthew H Porteus  
In:
(2021) 13: 598 
Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome
Categories:
Primary Cells and Media, Transfection 
Authors:
Valentina Vavassori , Elisabetta Mercuri , Genni E Marcovecchio , Maria C Castiello , Giulia Schiroli , Luisa Albano , Carrie Margulies , Frank Buquicchio , Elena Fontana , Stefano Beretta , Ivan Merelli , Andrea Cappelleri , Paola Mv Rancoita , Vassilios Lougaris , Alessandro Plebani , Maria Kanariou , Arjan Lankester , Francesca Ferrua , Eugenio Scanziani , Cecilia Cotta-Ramusino , Anna Villa , Luigi Naldini , Pietro Genovese  
In:
EMBO Mol Med (2021) 13(3): e13545 
Precise and error-prone CRISPR-directed gene editing activity in human CD34+ cells varies widely among patient samples
Categories:
Transfection 
Authors:
Shirin R Modarai , Sambee Kanda , Kevin Bloh , Lynn M Opdenaker , Eric B Kmiec  
In:
Gene Ther (2020) :  
CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Goodwin M, Lee E, Lakshmanan U, Shipp S, Froessl L, Barzaghi F, Passerini L, Narula M, Sheikali A, Lee CM, Bao G, Bauer CS, Miller HK, Garcia-Lloret M, Butte MJ, Bertaina A, Shah A, Pavel-Dinu M, Hendel A, Porteus M, Roncarolo MG, Bacchetta R. 
In:
Science (2020) 6(19): eaaz0571 
Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Jeffrey Sebrow , Stephen P Goff, Daniel O Griffin              
In:
Virol J (2020) 17: 1 
Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents
Categories:
Primary Cells and Media 
Authors:
Bhavna Verma , Amy Wesa  
In:
Curr Protoc Immunol (2020) 89: 1 
Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A. 
In:
Nat Biotechnol (2019) 1: 1 
Highly efficient editing of the ß-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease
Categories:
Transfection 
Authors:
So Hyun Park , Ciaran M Lee , Daniel P Dever , Timothy H Davis , Joab Camarena , Waracharee Srifa , Yankai Zhang , Alireza Paikari , Alicia K Chang , Matthew H Porteus , Vivien A Sheehan , Gang Bao  
In:
Nucleic Acids Res (2019) 47(15): 7955-7972 
Gene correction for SCID-X1 in long-term hematopoietic stem cells.
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Pavel-Dinu M, Wiebking V, Dejene BT, Srifa W, Mantri S, Nicolas CE, Lee C, Bao G, Kildebeck EJ, Punjya N, Sindhu C, Inlay MA, Saxena N, DeRavin SS, Malech H, Roncarolo MG, Weinberg KI, Porteus MH 
In:
Nat Commun. (2019) 10 (1): 1634 
Gene-edited stem cells enable CD33-directed immune therapy for myeloid malignancies.
Categories:
Transfection 
Authors:
Borot F, Wang H, Ma Y, Jafarov T, Raza A, Ali AM, Mukherjee S. 
In:
Proc Natl Acad Sci USA (2019) 116(24): 11978-11987 
Highly efficient therapeutic gene editing of human hematopoietic stem cells
Categories:
Serum-free and Speciality Media, Transfection 
Authors:
Wu Y, Zeng J, Roscoe BP, Liu P, Yao Q, Lazzarotto CR, Clement K, Cole MA, Luk K, Baricordi C, Shen AH, Ren C, Esrick EB, Manis JP, Dorfman DM, Williams DA, Biffi A, Brugnara C, Biasco L, Brendel C, Pinello L, Tsai SQ, Wolfe SA, Bauer DE. 
In:
Nat Med (2019) 25(5): 776-783 
Bacteria-free minicircle DNA system to generate integration-free CAR-T cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells.
Categories:
Transfection 
Authors:
Ting PY, Parker AE, Lee JS, Trussell C, Sharif O, Luna F, Federe G, Barnes SW, Walker JR, Vance J, Gao MY, Klock HE, Clarkson S, Russ C, Miraglia LJ, Cooke MP, Boitano AE, McNamara P, Lamb J, Schmedt C, Snead JL. 
In:
Nat Methods (2018) 15(11):  
A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Vakulskas CA, Dever DP, Rettig GR, Turk R, Jacobi AM, Collingwood MA, Bode NM, McNeill MS, Yan S, Camarena J, Lee CM, Park SH, Wiebking V, Bak RO, Gomez-Ospina N, Pavel-Dinu M, Sun W6, Bao G, Porteus MH, Behlke MA 
In:
Nat Med (2018) 24(8): 1216-1224 
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.
Categories:
Primary Cells and Media, Transfection 
Authors:
Farboud B1, Jarvis E2, Roth TL3, Shin J4, Corn JE4, Marson A5, Meyer BJ1, Patel NH6, Hochstrasser ML7. 
In:
J Vis Exp (2018) 2018: 135 
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions.
Categories:
Primary Cells and Media 
Authors:
Makar T, Nimmagadda VK, Guda PR, Hampton B, Huang W, Kane MA, Fishman PS, Pessac B, Bever CT Jr, Trisler D 
In:
Stem Cell Res Ther (2018) 1: 127 
Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors
Categories:
Primary Cells and Media, Transfection 
Authors:
Marta Holstein, Cristina Mesa-Nuñez, Csaba Miskey, Elena Almarza, Valentina Poletti, Marco Schmeer, Esther Grueso, Juan Carlos Ordóñez Flores, Dennis Kobelt, Wolfgang Walther, Manish K. Aneja, Johannes Geiger, Halvard B. Bonig, Zsuzsanna Izsvák, Martin Schleef, Carsten Rudolph, Fulvio Mavilio,Juan A. Bueren, Guillermo Guenechea, and Zoltán Ivics 
In:
Mol Ther (2018) 26(4): 1137-1153 
CRISPR/Cas9 genome editing in human hematopoietic stem cells.
Categories:
Primary Cells and Media 
Authors:
Bak RO, Dever DP, Porteus MH 
In:
Nat Protocols (2018) 13(2): 358-376 
Preclinical modeling highlights the therapeutic potential of hematopoietic stem cell gene editing for correction of SCID-X1
Categories:
Transfection 
Authors:
Schiroli G, Ferrari S, Conway A, Jacob A, Capo V, Albano L, Plati T, Castiello MC, Sanvito F, Gennery AR, Bovolenta C, Palchaudhuri R, Scadden DT, Holmes MC, Villa A, Sitia G, Lombardo A, Genovese P, Naldini L 
In:
Science (2017) 9(411): eaan0820 
Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.
Categories:
Transfection 
Authors:
Bak RO, Porteus MH. 
In:
Cell Rep (2017) 20(3): 750-756 
Marker-free coselection for CRISPR-driven genome editing in human cells.
Categories:
Transfection 
Authors:
Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganière J, Doyon Y. 
In:
Nat Methods (2017) 14(6): 615-620 
Long-Term Engraftment and Fetal Globin Induction upon BCL11A Gene Editing in Bone-Marrow-Derived CD34+ Hematopoietic Stem and Progenitor Cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Chang KH, Smith SE, Sullivan T, Chen K, Zhou Q, West JA, Liu M, Liu Y, Vieira BF, Sun C, Hong VP, Zhang M, Yang X, Reik A2, Urnov FD, Rebar EJ, Holmes MC, Danos O, Jiang H, Tan S 
In:
Mol Ther Methods Clin Dev. (2017) 4: 137-148 
CRISPR/Cas9 ß-globin gene targeting in human haematopoietic stem cells.
Categories:
Transfection 
Authors:
Dever DP, Bak RO, Reinisch A, Camarena J, Washington G, Nicolas CE, Pavel-Dinu M, Saxena N, Wilkens AB, Mantri S, Uchida N, Hendel A, Narla A, Majeti R, Weinberg KI, Porteus MH. 
In:
Nature (2016) 539(7629): 384-389 
Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.
Categories:
Transfection 
Authors:
DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D, Martin DI, Corn JE. 
In:
Science (2016) 8 (360): 1-9 
Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration
Categories:
Serum-free and Speciality Media 
Authors:
Griffin DO, Goff SP 
In:
Retrovirology (2016) 13: 14 
Niche-Based Screening in Multiple Myeloma Identifies a Kinesin-5 Inhibitor with Improved Selectivity over Hematopoietic Progenitors
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chattopadhyay S, Stewart AL, Mukherjee S, Huang C, Hartwell KA, Miller PG, Subramanian R, Carmody LC, Yusuf RZ, Sykes DB, Paulk , Vetere A, Vallet S, Santo L, Cirstea DD, Hideshima T, Dancík V, Majireck MM, Hussain MM, Singh S, Quiroz R, Iaconelli J, Karmacharya R, Tolliday NJ, Clemons PA, Moore MA, Stern AM, Shamji AF, Ebert BL, Golub TR, Raje NS, Scadden DT, Schreiber SL. 
In:
Cell Rep (2015) 15: 755-770 
cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Baghbaderani BA, Tian X, Neo BH, Burkall A, Dimezzo T, Sierra G, Zeng X, Warren K, Kovarcik DP, Fellner T, Rao MS 
In:
Stem Cell Reports (2015) 5(4): 647-659 
Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor Cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Breese EH, Buechele C, Dawson C, Cleary ML, Porteus MH. 
In:
PLoS ONE (2015) 10(9: e0136644 
Biphasic Effects of Ingenol 3,20-Dibenzoate on the Erythropoietin Receptor: Synergism at Low Doses and Antagonism at High Doses.
Categories:
Primary Cells and Media 
Authors:
Oh JG, Chin YW, Kim SJ, Choi JM, Kim SK, Kang HE, Heo TH 
In:
Mol Pharmacol (2015) 88(2): 392-400 
Immuno-modification of enhancing stem cells targeting for myocardial repair.
Categories:
Primary Cells and Media 
Authors:
Yu J, Wu YK, Gu Y, Fang Q, Sievers R, Ding CH, Olgin JE, Lee RJ 
In:
J Cell Mol Med (2015) 19(7): 1483-91 
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn, Matthew H Porteus 
In:
Nat Biotechnol (2015) 10.1038: nbt.3290 
MYB controls erythroid versus megakaryocyte lineage fate decision through the miR-486-3p-mediated downregulation of MAF.
Categories:
Transfection 
Authors:
Bianchi E, Bulgarelli J, Ruberti S, Rontauroli S, Sacchi G, Norfo R, Pennucci V, Zini R, Salati S, Prudente Z, Ferrari S, Manfredini R. 
In:
Cell Death Differ (2015) 1: 1-16 
Melanocortins Contribute to Sequential Differentiation and Enucleation of Human Erythroblasts via Melanocortin Receptors 1, 2 and 5
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Eriko Simamura1, Tomohiro Arikawa2, Takayuki Ikeda, Hiroki Shimada, Hiroki Shoji, Hiroko Masuta, Yuriko Nakajima, Hiroki Otani, Hideto Yonekura, Toshihisa Hatta 
In:
PLoS ONE (2015) 10: e0123232 
Activating c-KIT mutations confer oncogenic cooperativity and rescue RUNX1/ETO-induced DNA damage and apoptosis in human primary CD34+ hematopoietic progenitors.
Categories:
Primary Cells and Media 
Authors:
Wichmann C, Quagliano-Lo Coco I, Yildiz Ö, Chen-Wichmann L, Weber H, Syzonenko T, Döring C, Brendel C, Ponnusamy K, Kinner A, Brandts C, Henschler R, Grez M 
In:
Leukemia (2015) 29(2): 279-89 
Pharmacological properties of JTE-052: a novel potent JAK inhibitor that suppresses various inflammatory responses in vitro and in vivo.
Categories:
Primary Cells and Media 
Authors:
Tanimoto A, Ogawa Y, Oki C, Kimoto Y, Nozawa K, Amano W, Noji S, Shiozaki M, Matsuo A, Shinozaki Y, Matsushita M 
In:
Other (2015) 64(1): 41-51 
UMG Lenti: novel lentiviral vectors for efficient transgene- and reporter gene expression in human early hematopoietic progenitors.
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Chiarella E, Carrà G, Scicchitano S, Codispoti B, Mega T, Lupia M, Pelaggi D, Marafioti MG, Aloisio A, Giordano M, Nappo G, Spoleti CB, Grillone T, Giovannone ED, Spina R, Bernaudo F, Moore MA, Bond HM, Mesuraca M, Morrone G 
In:
PLoS ONE (2014) 9(12): ePub 
Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9
Categories:
Transfection 
Authors:
Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS, Stortchevoi A, Bryder D, Musunuru K, Brand H, Tager AM, Allen TM, Talkowski ME, Rossi DJ, Cowan CA 
In:
Cell Stem Cell (2014) 15(5): 643-652 
ELMO1 Is Upregulated in AML CD34+ Stem/Progenitor Cells, Mediates Chemotaxis and Predicts Poor Prognosis in Normal Karyotype AML
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Capala ME, Vellenga E, Schuringa JJ 
In:
PLoS ONE (2014) 10: e111568 
miRNA-mRNA integrative analysis in primary myelofibrosis CD34+ cells: role of miR-155/JARID2 axis in abnormal megakaryopoiesis.
Categories:
Transfection 
Authors:
Norfo R1, Zini R1, Pennucci V1, Bianchi E1, Salati S1, Guglielmelli P2, Bogani C2, Fanelli T2, Mannarelli C2, Rosti V3, Pietra D4, Salmoiraghi S5, Bisognin A6, Ruberti S1, Rontauroli S1, Sacchi G1, Prudente Z1, Barosi G3, Cazzola M4, Rambaldi A5, Bortoluzzi S6, Ferrari S7, Tagliafico E7, Vannucchi AM2, Manfredini R1; Associazione Italiana per la Ricerca sul Cancro Gruppo Italiano Malattie Mieloproliferative Investigators. 
In:
Blood (2014) 124 (13): e21-32 
Hydroxyurea-inducible SAR1 gene acts through the Gia/JNK/Jun pathway to regulate ?-globin expression.
Categories:
Primary Cells and Media, Transfection 
Authors:
Zhu J, Chin K, Aerbajinai W, Kumkhaek C, Li H, Rodgers GP 
In:
Blood (2014) 124(7): 1146-56 
Dual-regulated lentiviral vector for gene therapy of X-linked chronic granulomatosis.
Categories:
Primary Cells and Media 
Authors:
Chiriaco M, Farinelli G, Capo V, Zonari E, Scaramuzza S, Di Matteo G, Sergi LS, Migliavacca M, Hernandez RJ, Bombelli F, Giorda E, Kajaste-Rudnitski A, Trono D, Grez M, Rossi P, Finocchi A, Naldini L, Gentner B, Aiuti A 
In:
Mol Ther (2014) 22(8): 1472-83 
Targeted genome editing in human repopulating haematopoietic stem cells
Categories:
Transfection 
Authors:
Genovese P, Schiroli G, Escobar G, Di Tomaso T1, Firrito C, Calabria A, Moi D, Mazzieri R, Bonini C, Holmes MC, Gregory PD, van der Burg M, Gentner B, Montini E, Lombardo A, Naldini L. 
In:
Nature (2014) 510: 235-40 
Genome editing for human gene therapy.
Categories:
Primary Cells and Media, Transfection 
Authors:
Meissner TB1, Mandal PK2, Ferreira LM1, Rossi DJ3, Cowan CA4. 
In:
Methods Enzymol (2014) 546: 273-95 
Antagonism of SET using OP449 enhances the efficacy of tyrosine kinase inhibitors and overcomes drug resistance in myeloid leukemia.
Categories:
Primary Cells and Media 
Authors:
Agarwal A, MacKenzie RJ, Pippa R, Eide CA, Oddo J, Tyner JW, Sears R, Vitek MP, Odero MD, Christensen DJ, Druker BJ 
In:
Clin Cancer Res (2014) 20(8): 2092-103 
Convergence of hypoxia and TGFß pathways on cell cycle regulation in human hematopoietic stem/progenitor cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wierenga AT, Vellenga E, Schuringa JJ 
In:
PLoS ONE (2014) 9(3): e93494 
NOD-Rag2null IL-2R?null mice: an alternative to NOG mice for generation of humanized mice.
Categories:
Primary Cells and Media 
Authors:
Katano I, Ito R, Kamisako T, Eto T, Ogura T, Kawai K, Suemizu H, Takahashi T, Kawakami Y, Ito M 
In:
Other (2014) 63(3): 321-30 
Loss of quiescence and impaired function of CD34(+)/CD38(low) cells one year following autologous stem cell transplantation
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Woolthuis CM, Brouwers-Vos AZ, Huls G, de Wolf JT, Schuringa JJ, Vellenga E 
In:
Haematologica (2013) 98(12): 1964-71 
ß-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model.
Categories:
Primary Cells and Media, Transfection 
Authors:
Sjeklocha LM, Wong PY, Belcher JD, Vercellotti GM, Steer CJ 
In:
PLoS ONE (2013) 8(11): e80403 
Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
A unique anti-CD115 monoclonal antibody which inhibits osteolysis and skews human monocyte differentiation from M2-polarized macrophages toward dendritic cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Haegel H, Thioudellet C, Hallet R, Geist M, Menguy T, Le Pogam F, Marchand JB, Toh ML, Duong V, Calcei A, Settelen N, Preville X, Hennequi M, Grellier B, Ancian P, Rissanen J, Clayette P, Guillen C, Rooke R, Bonnefoy JY. 
In:
Other (2013) 5(5): 736-747 
Regulation of cell shape and adhesion by CD34.
Categories:
Primary Cells and Media 
Authors:
Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K 
In:
Cell Adh Migr (2013) 7(5): 426-33 
Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.
Categories:
Primary Cells and Media 
Authors:
Qin Q, Penkert RR, Kalejta RF 
In:
J Virol (2013) 87(17): 9886-94 
Extending human hematopoietic stem cell survival in vitro with adipocytes.
Categories:
 
Authors:
Glettig DL, Kaplan DL. 
In:
BioResearch Open Access (2013) 2(3): 179-85 
Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34+ cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merling RK1, Sweeney CL, Choi U, De Ravin SS, Myers TG, Otaizo-Carrasquero F, Pan J, Linton G, Chen L, Koontz S, Theobald NL, Malech HL. 
In:
Blood (2013) 121(14): e98-107 
The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Poole E, Walther A, Raven K, Benedict CA, Mason GM, Sinclair J. 
In:
J Virol (2013) 87(8): 4261-71 
Evidence of a Hematopoietic Origin of Human Bone Marrow Stromal Cells
Categories:
Primary Cells and Media 
Authors:
Milwid JM, Li M,USA LeeM J, Yarmush ML, Parekkadan B 
In:
Other (2013) 1(2): 1000113 
A survey of ex vivo/in vitro transduction efficiency of mammalian primary cells and cell lines with Nine natural adeno-associated virus (AAV1-9) and one engineered adeno-associated virus serotype.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ellis BL, Hirsch ML, Barker JC, Connelly JP, Steininger RJ 3rd, Porteus MH. 
In:
Virol J (2013) 10: 74 
Preclinical safety and efficacy of human CD34(+) cells transduced with lentiviral vector for the treatment of Wiskott-Aldrich syndrome.
Categories:
Primary Cells and Media 
Authors:
Scaramuzza S, Biasco L, Ripamonti A, Castiello MC, Loperfido M, Draghici E, Hernandez RJ, Benedicenti F, Radrizzani M, Salomoni M, Ranzani M, Bartholomae CC, Vicenzi E, Finocchi A, Bredius R, Bosticardo M, Schmidt M, von Kalle C, Montini E, Biffi A, Roncarolo MG, Naldini L, Villa A, Aiuti A 
In:
Mol Ther (2013) 21(1): 175-84 
Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jovin IS, Lei L, Huang Y, Hao Z, Curtis JP, Brennan JJ, Remetz MS, Setaro JF, Pfau SE, Howes CJ, Clancy JF, Cabin HS, Cleman MW, Giordano FJ 
In:
J Cell Mol Med (2012) 16(12): 3022-7 
A sensitive and quantitative polymerase chain reaction-based cell free in vitro non-homologous end joining assay for hematopoietic stem cells.
Categories:
Primary Cells and Media 
Authors:
Shao L, Feng W, Lee KJ, Chen BP, Zhou D 
In:
PLoS ONE (2012) 7(3): e33499 
Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke.
Categories:
Primary Cells and Media 
Authors:
Wagner DC, Bojko M, Peters M, Lorenz M, Voigt C, Kaminski A, Hasenclever D, Scholz M, Kranz A, Weise G, Boltze J. 
In:
Other (2012) 4(1): 17 
Local transplantation of ex vivo expanded bone marrow-derived CD34-positive cells accelerates fracture healing.
Categories:
Primary Cells and Media 
Authors:
Kawakami Y, Ii M, Alev C, Kawamoto A, Matsumoto T, Kuroda R, Shoji T, Fukui T, Masuda H, Akimaru H, Mifune Y, Kuroda T, Horii M, Yokoyama A, Kurosaka M, Asahara T 
In:
Cell Transplant (2012) 21(12): 2689-709 
Local Transplantation of Granulocyte Colony-Stimulating Factor-Mobilized Human Peripheral Blood Mononuclear Cells for Unhealing Bone Fractures
Categories:
Primary Cells and Media 
Authors:
Fukui T, Matsumoto T, Mifune Y, Shoji T, Kuroda T, Kawakami Y, Kawamoto A, Ii M, Kawamata S, Kurosaka M, Asahara T, Kuroda R. 
In:
Cell Transplant (2012) 21: 707-721 
Long-term effects of intravitreal injection of GMP-grade bone-marrow-derived CD34+ cells in NOD-SCID mice with acute ischemia-reperfusion injury.
Categories:
Primary Cells and Media 
Authors:
Park SS, Caballero S, Bauer G, Shibata B, Roth A, Fitzgerald PG, Forward KI, Zhou P, McGee J, Telander DG, Grant MB, Nolta JA. 
In:
Invest Ophthalmol Vis Sci (2012) 53(2): 986-94 
Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors
Categories:
Transfection 
Authors:
Mack AA, Kroboth S, Rajesh D, Wang WB 
In:
PLoS ONE (2011) 6 (11): e27956 
Resveratrol ameliorates TNFa-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-?B signalling.
Categories:
Primary Cells and Media 
Authors:
Jeong JY, Silver M, Parnes A, Nikiforow S, Berliner N, Vanasse GJ. 
In:
Br J of Haematol (2011) 155(1): 93-101 
Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34? progenitors.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Poole E, McGregor Dallas SR, Colston J, Joseph RS, Sinclair J 
In:
J Gen Virol (2011) 92(7): 1539-49 
Hematopoietic progenitors express embryonic stem cell and germ layer genes.
Categories:
Primary Cells and Media 
Authors:
Pessac B, Bara MA, Ford D, Patibandla GK, Trisler D 
In:
Other (2011) 334(4): 300-6 
Human induced pluripotent stem cells are capable of B-cell lymphopoiesis
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee Carpenter, Ram Malladi, Cheng-Tao Yang, Anna French, Katherine J. Pilkington, Richard W. Forsey, Jackie Sloane-Stanley, Kathryn M. Silk, Timothy J. Davies, Paul J. Fairchild, Tariq Enver, and Suzanne M. Watt 
In:
Blood (2011) 117(15): 4008-11 
Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.
Categories:
Transfection 
Authors:
Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA, Tong J, Cheng L. 
In:
Cell Res (2011) 21: 518-529 
The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and reduces pathological neovascularization associated with ischemic retinopathy
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hasan A, Pokeza N, Shaw L, Lee HS, Lazzaro D, Chintala H, Rosenbaum D, Grant MB, Chaqour B 
In:
J Biol Chem (2011) 286(11): 9542-54 
CD34+CD140b+ cells and circulating CXCL12 correlate with the angiographically assessed severity of cardiac allograft vasculopathy
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schober A, Hristov M, Kofler S, Forbrig R, Löhr B, Heussen N, Zhe Z, Akhtar S, Schumann U, Krötz F, Leibig M, König A, Kaczmarek I, Reichart B, Klauss V, Weber C, Sohn HY 
In:
Other (2011) 32(4): 476-84 
KRAS(G12V) enhances proliferation and initiates myelomonocytic differentiation in human stem/progenitor cells via intrinsic and extrinsic pathways
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fatrai S, van Gosliga D, Han L, Daenen SM, Vellenga E, Schuringa JJ 
In:
J Biol Chem (2011) 286(8): 6061-70 
Differential expression of microRNAs in CD34+ cells of 5q- syndrome
Categories:
Primary Cells and Media 
Authors:
Votavova H, Grmanova M, Dostalova Merkerova M, Belickova M, Vasikova A, Neuwirtova R, Cermak J 
In:
J Hematol Oncol (2011) 6(4): 1 
c-myb supports erythropoiesis through the transactivation of KLF1 and LMO2 expression.
Categories:
Transfection 
Authors:
Bianchi E, Zini R, Salati S, Tenedini E, Norfo R, Tagliafico E, Manfredini R, Ferrari S. 
In:
Blood (2010) 116(22): 99-110 
The role of STAT5 and KRAS in hematopoiesis and acute myeoloid leukemia
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fátrai S 
In:
Other (2010) 0:0: Thesis 
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo
Categories:
Transfection 
Authors:
Holt N, Wang J, Kim K, Friedman G, Wang X, Taupin V, Crooks GM, Kohn DB, Gregory PD, Holmes MC, Cannon PM. 
In:
Nat Biotechnol (2010) 28: 839-847 
An in-vitro Adhesion Pattern study of Hematopoietic Stem Cells on Porous Titanium and Bioceramic Scaffolds
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rajyalakshmi A, Balasubramanian K, Mishra S 
In:
Other (2010) 24(2): 116-122 
Cellular and viral control over the initial events of human cytomegalovirus experimental latency in CD34+ cells.
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Saffert RT, Penkert RR, Kalejta RF 
In:
J Virol (2010) 84(11): 5594-604 
Induction of reactive oxygen species by human T-cell leukemia virus type 1 tax correlates with DNA damage and expression of cellular senescence marker
Categories:
 
Authors:
Kinjo T, Ham-Terhune J, Peloponese JM Jr, Jeang KT 
In:
J Virol (2010) 84(10): 5431-7 
Transduction of Human Primitive Repopulating Hematopoietic Cells With Lentiviral Vectors Pseudotyped With Various Envelope Proteins
Categories:
Primary Cells and Media 
Authors:
Kim YS, Wielgosz MM, Hargrove P, Kepes S, Gray J, Persons DA, Nienhuis AW 
In:
Mol Ther (2010) 18(7): 1310-1317 
A zinc-finger transcriptional activator designed to interact with the gamma-globin gene promoters enhances fetal hemoglobin production in primary human adult erythroblasts.
Categories:
Primary Cells and Media 
Authors:
Wilber A, Tschulena U, Hargrove PW, Kim YS, Persons DA, Barbas CF 3rd, Nienhuis AW. 
In:
Blood (2010) 115(15): 3033-41 
Induction of nitric oxide by erythropoietin is mediated by the common receptor and requires interaction with VEGF receptor 2
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sautina L, Sautin Y, Beem E, Zhou Z, Schuler A, Brennan J, Zharikov SI, Diao Y, Bungert J, Segal MS 
In:
Blood (2010) 115(4): 896-905 
Local Transplantation of Ex-Vivo Expanded Bone Marrow CD34-Positive Cells for Rat Non-Union Fracture Model
Categories:
Primary Cells and Media 
Authors:
Kawakami Y, Shoji T, Kawamoto A, Matsumoto T, Mifune Y, Fukui T, Kuroda R, Kurosaka M, Asahara T 
In:
Other (2010) 0:0: Whitepaper 
Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.
Categories:
Primary Cells and Media 
Authors:
Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L 
In:
Blood (2009) 114(27): 5473-80 
The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose.
Categories:
Primary Cells and Media 
Authors:
Scarfì S, Fresia C, Ferraris C, Bruzzone S, Fruscione F, Usai C, Benvenuto F, Magnone M, Podestà M, Sturla L, Guida L, Albanesi E, Damonte G, Salis A, De Flora A, Zocchi E. 
In:
Stem Cells (2009) 27(10): 2469-77 
Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system.
Categories:
Transfection 
Authors:
Xue X, Huang X, Nodland SE, Mátés L, Ma L, Izsvák Z, Ivics Z, LeBien TW, McIvor RS, Wagner JE, Zhou X. 
In:
Blood (2009) 114(7): 1319-30 
Repression of BMI1 in normal and leukemic human CD34(+) cells impairs self-renewal and induces apoptosis
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizo A, Olthof S, Han L, Vellenga E, de Haan G, Schuringa JJ 
In:
Blood (2009) 114(8): 1498-505 
Establishing EGFP congenic mice in a NOD/Shi-scid IL2Rg(null) (NOG) genetic background using a marker-assisted selection protocol (MASP)
Categories:
Primary Cells and Media, Electrophoreses and Analysis  
Authors:
Suemizu H, Yagihashi C, Mizushima T, Ogura T, Etoh T, Kawai K, Ito M 
In:
Other (2008) 57(5): 471-7 
Interferon-alpha 2b-induced thrombocytopenia is caused by inhibition of platelet production but not proliferation and endomitosis in human megakaryocytes.
Categories:
Primary Cells and Media 
Authors:
Yamane A, Nakamura T, Suzuki H, Ito M, Ohnishi Y, Ikeda Y, Miyakawa Y. 
In:
Blood (2008) 112(3): 542-50 
Downregulation of signal transducer and activator of transcription 5 (STAT5) in CD34+ cells promotes megakaryocytic development, whereas activation of STAT5 drives erythropoiesis
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Olthof SG, Fatrai S, Drayer AL, Tyl MR, Vellenga E, Schuringa JJ 
In:
Stem Cells (2008) 26(7): 1732-42 
Local delivery of granulocyte colony stimulating factor-mobilized CD34-positive progenitor cells using bioscaffold for modality of unhealing bone fracture.
Categories:
Primary Cells and Media 
Authors:
Mifune Y, Matsumoto T, Kawamoto A, Kuroda R, Shoji T, Iwasaki H, Kwon SM, Miwa M, Kurosaka M, Asahara T 
In:
Stem Cells (2008) 26(6): 1395-405 
Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation.
Categories:
Primary Cells and Media 
Authors:
Zhang CC, Kaba M, Iizuka S, Huynh H, Lodish HF. 
In:
Blood (2008) 111(7): 3415-23 
Long-term maintenance of human hematopoietic stem/progenitor cells by expression of BMI1
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizo A, Dontje B, Vellenga E, de Haan G, Schuringa JJ 
In:
Blood (2008) 111(5): 2621-30 
Administrations of peripheral blood CD34-positive cells contribute to medial collateral ligament healing via vasculogenesis.
Categories:
Primary Cells and Media 
Authors:
Tei K, Matsumoto T, Mifune Y, Ishida K, Sasaki K, Shoji T, Kubo S, Kawamoto A, Asahara T, Kurosaka M, Kuroda R 
In:
Stem Cells (2008) 26(3): 819-30 
Reduced activation of protein kinase B, Rac, and F-actin polymerization contributes to an impairment of stromal cell derived factor-1 induced migration of CD34+ cells from patients with myelodysplasia
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fuhler GM, Drayer AL, Olthof SG, Schuringa JJ, Coffer PJ, Vellenga E 
In:
Blood (2008) 111(1): 359-68 
Target proteins of C/EBPp30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9
Categories:
Transfection 
Authors:
Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre G 
In:
Blood (2007) 110(9): 3301-9 
The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription
Categories:
Transfection 
Authors:
Tanabe O, Shen Y, Liu Q, Campbell AD, Kuroha T, Yamamoto M, Engel JD 
In:
Genes Dev (2007) 21(21): 2832-2844 
STAT5 is required for long-term maintenance of normal and leukemic human stem/progenitor cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schepers H, van Gosliga D, Wierenga AT, Eggen BJ, Schuringa JJ, Vellenga E 
In:
Blood (2007) 110(8): 2880-8 
Protein Phosphatase 2A Plays an Important Role in Stromal Cell-Derived Factor-1/CXC Chemokine Ligand 12-Mediated Migration and Adhesion of CD34+ Cells
Categories:
Transfection 
Authors:
Basu S, Ray NT, Atkinson SJ, Broxmeyer HE 
In:
J Immunol (2007) 179(5): 3075-85 
5-Androstenediol promotes survival of gamma-irradiated human hematopoietic progenitors through induction of nuclear factor-kappaB activation and granulocyte colony-stimulating factor expression
Categories:
Transfection 
Authors:
Xiao M, Inal CE, Parekh VI, Chang CM, Whitnall MH 
In:
Mol Pharmacol (2007) 72(2): 370-9 
Reintroduction of C/EBPalpha in leukemic CD34+ stem/progenitor cells impairs self-renewal and partially restores myelopoiesis
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schepers H, Wierenga AT, van Gosliga D, Eggen BJ, Vellenga E, Schuringa JJ 
In:
Blood (2007) 110(4): 1317-25 
mRNA-Mediated Gene Delivery Into Human Progenitor Cells Promotes Highly Efficient Protein Expression
Categories:
Transfection 
Authors:
Wiehe JM, Ponsaerts P, Rojewski MT, Homann JM, Greiner J, Kronawitter D, Schrezenmeier H, Hombach V, Wiesneth M, Zimmermann O, Torzewski J 
In:
J Cell Mol Med (2007) 11(3): 521-30 
Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1).
Categories:
Primary Cells and Media 
Authors:
Alcantara O, Boldt DH 
In:
Br J of Haematol (2007) 137(3): 252-61 
c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression
Categories:
Transfection 
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM 
In:
Mol Cell Biol (2007) 27(6): 2048-58 
Local Transplantation of G-CSF Mobilized CD34-Positive Cells Contributes to Fracture Healing Via Vasculogenesis and Osteogenesis
Categories:
Primary Cells and Media 
Authors:
Mifune Y, Matsumoto T, Kawamoto A, Kuroda R, Iwasaki H, Shoji T, Lee SY, Oyamada A, Horii M, Kurosaka M,Asahara T 
In:
Other (2007) 0:0: Whitepaper 
Proteomic discovery of Max as a novel interacting partner of C/EBPalpha: a Myc/Max/Mad link
Categories:
Transfection 
Authors:
Zada AA, Pulikkan JA, Bararia D, Geletu M, Trivedi AK, Balkhi MY, Hiddemann WD, Tenen DG, Behre HM, Behre G 
In:
Leukemia (2006) 20(12): 2137-2146 
Mechanism for fetal hemoglobin induction by histone deacetylase inhibitors involves gamma-globin activation by CREB1 and ATF-2
Categories:
Transfection 
Authors:
Sangerman J, Lee MS, Yao X, Oteng E, Hsiao CH, Li W, Zein S, Ofori-Acquah SF, Pace BS 
In:
Blood (2006) 108(10): 3590-9 
Identification of Notch target genes in uncommitted T-cell progenitors: no direct induction of a T-cell specific gene program
Categories:
Transfection 
Authors:
Weerkamp F, Luis TC, Naber BA, Koster EE, Jeannotte L, van Dongen JJ, Staal FJ 
In:
Leukemia (2006) 20(11): 1967-77 
Differential effects of recombinant thrombopoietin and bone marrow stromal-conditioned media on neonatal versus adult megakaryocytes
Categories:
Primary Cells and Media 
Authors:
Pastos KM, Slayton WB, Rimsza LM, Young L, Sola-Visner MC 
In:
Blood (2006) 108(10): 3360-2 
Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells
Categories:
Transfection 
Authors:
Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S 
In:
Cell Death Differ (2006) 13(10): 1686-96 
Gene therapy for BCR-ABL+ human CML with dual phosphorylation resistant p27Kip1 and stable RNA interference using an EBV vector
Categories:
Transfection 
Authors:
Sengupta A, Banerjee D, Chandra S, Banerjee S 
In:
J Gene Med (2006) 8(10): 1251-61 
Stable gene transfer to human CD34(+) hematopoietic cells using the Sleeping Beauty transposon
Categories:
Transfection 
Authors:
Hollis RP, Nightingale SJ, Wang X, Pepper KA, Yu XJ, Barsky L, Crooks GM, Kohn DB 
In:
Exp Hematol (2006) 34(10): 1333-43 
NUP98-HOXA9 Induces Long-term Proliferation and Blocks Differentiation of Primary Human CD34+ Hematopoietic Cells
Categories:
Transfection 
Authors:
Takeda A, Goolsby C, Yaseen NR 
In:
Cancer Res (2006) 66(13): 6628-37 
Integrin inhibition through Lyn-dependent cross talk from CXCR4 chemokine receptors in normal human CD34+ marrow cells
Categories:
Transfection 
Authors:
Nakata Y, Tomkowicz B, Gewirtz AM, Ptasznik A 
In:
Blood (2006) 107(11): 4234-9 
Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway
Categories:
Transfection 
Authors:
He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J 
In:
Cell (2006) 125(5): 929-41 
PI3K/Akt-dependent Epo-induced signalling and target genes in human early erythroid progenitor cells
Categories:
Transfection 
Authors:
Sivertsen EA, Hystad ME, Gutzkow KB, Dosen G, Smeland EB, Blomhoff HK, Myklebust JH 
In:
Br J of Haematol (2006) 135(1): 117-28 
Nucleofection, an efficient nonviral method to transfer genes into human hematopoietic stem and progenitor cells
Categories:
Transfection 
Authors:
von Levetzow G, Spanholtz J, Beckmann J, Fischer J, Kogler G, Wernet P, Punzel M, Giebel B 
In:
Stem Cells Dev (2006) 15(2): 278-85 
Erythroid Inhibition by the Leukemic Fusion AML1-ETO Is Associated with Impaired Acetylation of the Major Erythroid Transcription Factor GATA-1
Categories:
Transfection 
Authors:
Choi Y, Elagib KE, Delehanty LL, Goldfarb AN 
In:
Cancer Res (2006) 66(6): 2990-6 
Efficient transient genetic labeling of human CD34+ progenitor cells for in vivo application
Categories:
Transfection 
Authors:
Wiehe JMI, Niesler C, Torzowski J, Zimmermann O, Wiesneth M, Schmitt M, Schwarz K, Dohner H, Hombach V Greiner J 
In:
Regenerative Med (2006) 1(2): 223-234 
cAMP-induced PKC activation increases functional CXCR4 expression on human CD34+ hematopoietic progenitors
Categories:
Transfection 
Authors:
Goichberg P, Kalinkovich A, Borodovsky N, Tesio M, Petit I, Nagler A, Hardan I and Lapidot T 
In:
Blood (2006) 107(3): 870-9 
PKCepsilon controls protection against TRAIL in erythroid progenitors
Categories:
Transfection 
Authors:
Mirandola P, Gobbi G, Ponti C, Sponzilli I, Cocco L and Vitale M 
In:
Blood (2006) 107(2): 508-513 
2'-deoxy-2'-fluoro-beta-D-arabinonucleic acid (2'F-ANA) modified oligonucleotides (ON) effect highly efficient, and persistent, gene silencing
Categories:
Transfection 
Authors:
Kalota A, Karabon L, Swider CR, Viazovkina E, Elzagheid M, Damha MJ and Gewirtz AM 
In:
Nucleic Acids Res (2006) 34(2): 451-461 
Mammalian target of rapamycin is required for thrombopoietin-induced proliferation of megakaryocyte progenitors
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drayer AL, Olthof SG, Vellenga E 
In:
Stem Cells (2006) 24(1): 105-14 
The hydroxyurea-induced small GTP-binding protein SAR modulates -globin gene expression in human erythroid cells
Categories:
Transfection 
Authors:
Tang DC, Zhu J, Liu W, Chin K, Sun J, Chen L, Hanover JA and Rodgers GP 
In:
Blood (2005) 94(5): 3278-3291 
The transcription factor GATA-1 potently represses the expression of the HIV-1 co-receptor CCR5 in human T cells and dendritic cells
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
In:
Blood (2005) 106(10): 3440-3448 
Genetic modification of hematopoietic stem cells with nonviral systems: past progress and future prospects
Categories:
Transfection 
Authors:
Papapetrou EP, Zoumbos NC and Athanassiadou A 
In:
Gene Ther (2005) 12 Suppl 1: 118-130 
SATB1 family protein expressed during early erythroid differentiation modifies globin gene expression
Categories:
Transfection 
Authors:
Wen J, Huang S, Rogers H, Dickinson LA, Kohwi-Shigematsu T and Noguchi CT 
In:
Blood (2005) 105(8): 3330-3339 
PI3-kinase/Akt is constitutively active in primary acute myeloid leukaemia cells and regulates survival and chemoresistance via NF-kB, MAPkinase and p53 pathways
Categories:
Transfection 
Authors:
Grandage VL, Gale RE, Linch DC and Khwaja A 
In:
Leukemia (2005) 19(4): 586-594 
Constitutive activation of the Wnt/beta-catenin signalling pathway in acute myeloid leukaemia
Categories:
Transfection 
Authors:
Simon M, Grandage VL, Linch DC and Khwaja A 
In:
Oncogene (2005) 24(14): 2410-2420 
Breast cancer cells can evade apoptosis-mediated selective killing by a novel small molecule inhibitor of Bcl-2
Categories:
Transfection 
Authors:
Real PJ, Cao Y, Wang R, Nikolovska-Coleska Z, Sanz-Ortiz J, Wang S and Fernandez-Luna JL 
In:
Cancer Res (2004) 64(21): 7947-7953 
Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells
Categories:
Transfection 
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM 
In:
Nat Med (2004) 10(11): 1187-1189 
Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells
Categories:
Transfection 
Authors:
Giebel B, Corbeil D, Beckmann J, Hohn J, Freund D, Giesen K, Fischer J, Kogler G and Wernet P 
In:
Blood (2004) 104(8): 2332-2338 
Caspase-3 has a nonapoptotic function in erythroid maturation
Categories:
Transfection 
Authors:
Carlile GW, Smith DH, Wiedmann M. 
In:
Blood (2004) 103(11): 4310-4316 
Transient genetic labeling of human CD34-positive hematopoietic stem cells using nucleofection
Categories:
Transfection 
Authors:
Greiner J, Wiehe J, Wiesneth M, Zwaka TP, Prill T, Schwarz K, Bienek-Ziolkowski M, Schmitt M, Döhner H, Hombach V and Torzewski J 
In:
Transfus Med Hemother (2004) 31: 136-141 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Induction of megakaryocytic differentiation in primary human erythroblasts: a physiological basis for leukemic lineage plasticity
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Goldfarb AN, Wong D, Racke FK 
In:
Am J Pathol (2001) 158(4): 1191-8 
Differential regulation of responsiveness to fMLP and C5a upon dendritic cell maturation: correlation with receptor expression.
Categories:
Primary Cells and Media, Classical Media and Reagents 
Authors:
Yang D, Chen Q, Stoll S, Chen X, Howard OM, Oppenheim JJ 
In:
J Immunol (2000) 165(5): 2694-702 
Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang D, Chen Q, Chertov O, Oppenheim JJ. 
In:
J Leukoc Biol (2000) 68(1): 9-14 
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