T cell, human peripheral blood unstim.

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Peripheral blood lymphocytes; not pre-stimulated.
Peripheral Blood CD4+ T cells play a central role in regulating the cell-mediated immune response to infection. Often known as helper T cells, they act on other cells of the immune system to promote various aspects of the immune response, including immunoglobulin isotype switching and affinity maturation of the antibody response, macrophage activation and enhanced activity of natural killer (NK) cells and cytotoxic T cells (CTL). CD4+ T cells act by releasing cytokines in response to antigenic stimulation. The release of cytokines allows cells of different types to talk to each other in the on-going immune response. One of the major effector functions of the peripheral blood CD4+ T cells is in the activation of macrophages.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth
TheraPeak® T-VIVO®

TheraPeak® T-VIVO® Cell Culture Medium is optimized to support cell therapy applications ustilizing human T cells. 

Its novel chemically defined formulation is serum-free and non-animal origin (NAO) to ensure consistency and provide increased process control.  

Unlike other serum-free T-cell culture media, TheraPEAK® T-VIVO®  Cell Culture Medium does not require the addition of human serum (HS) to achieve the desired performance.

It dos not contain cytokines, antibiotics or phenol red

All TheraPEAK® Products are produced according to applicable GMP standards and follow the USP/EP guidance for cell and gene therapy raw materials. TheraPEAK® Media Products are produced at FDA registered manufacturing sites under the auspices of ISO 13485 certified Quality Management Systems.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

FI-115

 5e5 58-64% 52-90% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3

FI-115

 1e6 73-79% 67-89% Plasmid (general) 2 µg 100 µl 4D X-Unit
T cell, human U-014 5e6 55-77% Plasmid (general) 5 µg 100 µl I/II/2b
P3 96-FI-115 5e5 74-86% 46-60% Plasmid (general) 0.4 µg 20 µl 96-well
P3 96-EO-115 5e5 68% ±5 79% ±8 Plasmid (general) 0.4 µg 20 µl 96-well

Citations (207)

Mitigation of chromosome loss in clinical CRISPR Cas9-engineered T cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y, Hopkins CR, Parker KR, Qi Y, Satpathy AT, Stadtmauer EA, Cate JHD, Eyquem J, Fraietta JA, June CH, Chang HY, Ye CJ, Doudna JA 
In:
Cell Rep (2023) 03.22: 533709 
A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers
Categories:
Primary Cells and Media, Transfection 
Authors:
Derek P Wong , Nand K Roy , Keman Zhang , Anusha Anukanth, Abhishek Asthana , Nicole J Shirkey-Son , Samantha Dunmire , Bryan J Jones , Walker S Lahr , Beau R Webber , Branden S Moriarity , Paolo Caimi , Reshmi Parameswaran  
In:
Nat Commun. (2022) 13: 217 
Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4 + T cells allows complex functional analyses
Categories:
Transfection 
Authors:
Manuel Albanese, Adrian Ruhle, Jennifer Mittermaier, Ernesto Mejías-Pérez, Madeleine Gapp, Andreas Linder, Niklas A Schmacke, Katharina Hofmann, Alexandru A Hennrich, David N Levy, Andreas Humpe, Karl-Klaus Conzelmann, Veit Hornung, Oliver T Fackler, Oliver T Keppler 
In:
Nat Methods (2021) 1: 1-27 
Hepatitis Delta Virus Acts as an Immunogenic Adjuvant in Hepatitis B Virus-Infected Hepatocytes
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Christine Y.L. Tham, Janine Kah, Anthony T. Tan, ..., Marc L€utgehetmann, Maura Dandri, Antonio Bertoletti 
In:
Cell Rep (2020) 1 (4): 100060 
Phenotype of SARS-CoV-2-specific T-cells in COVID-19 patients with acute respiratory distress syndrome
Categories:
Classical Media and Reagents 
Authors:
Daniela Weiskopf, Katharina S. Schmitz, Matthijs P. Raadsen, Alba Grifoni, Nisreen M.A. Okba, Henrik Endeman, Johannes P.C. van den Akker, Richard Molenkamp, Marion P.G. Koopmans, Eric C.M. van Gorp, Bart L. Haagmans, Rik L. de Swart, Alessandro Sette & Rory D. de Vries 
In:
Science (2020) 5: 48 
Preclinical Activity of Embryonic Annexin A2-Specific Chimeric Antigen Receptor T Cells Against Ovarian Cancer
Categories:
Transfection 
Authors:
Leonard Leong , Heng Liang Tan , Simeon Cua , Kylie Su Mei Yong , Qingfeng Chen , Andre Choo  
In:
Int J Mol Sci (2020) 21(2): 381 
Closed-system transposon-mediated manufacture of GMP grade CAR T-cells via the Lonza Nucleofector LV XL
Categories:
Transfection 
Authors:
L.M. Brownrigg, S. Nichols, E. Bosio, B. Carnley, M. Sturm 
In:
Cytotherapy (2020) 22: S382 
Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Oh SA, Seki A, Rutz S.  
In:
Curr Protoc Immunol (2019) 124(1): e69 
Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR
Categories:
Primary Cells and Media, Transfection 
Authors:
Hamada M1, Nishio N2, Okuno Y3, Suzuki S3, Kawashima N1, Muramatsu H1, Tsubota S4, Wilson MH5, Morita D6, Kataoka S1, Ichikawa D1, Murakami N1, Taniguchi R1, Suzuki K1, Kojima D1, Sekiya Y1, Nishikawa E1, Narita A1, Hama A1, Kojima S1, Nakazawa Y6, Takahashi Y7. 
In:
EBioMedicine (2018) 2352:  
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Seki A, Rutz S. 
In:
J Exp Med (2018) 215(3): 985-997 
Improved Expansion and In Vivo Function of Patient T Cells by a Serum-free Medium.
Categories:
Serum-free and Speciality Media 
Authors:
Medvec AR, Ecker C, Kong H, Winters EA, Glover J, Varela-Rohena A, Riley JL.  
In:
Mol Ther Methods Clin Dev. (2017) 7; 8: 65-74 
Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors.
Categories:
Transfection 
Authors:
Monjezi R1, Miskey C2, Gogishvili T1, Schleef M3, Schmeer M3, Einsele H1, Ivics Z2, Hudecek M1. 
In:
Leukemia (2016) 10: epub 
Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells.
Categories:
Transfection 
Authors:
Kebriaei P, Singh H, Huls MH, Figliola MJ, Bassett R, Olivares S, Jena B, Dawson MJ, Kumaresan PR, Su S, Maiti S, Dai J, Moriarity B, Forget MA, Senyukov V, Orozco A, Liu T, McCarty J, Jackson RN, Moyes JS, Rondon G, Qazilbash M, Ciurea S, Alousi A, Nieto Y, Rezvani K, Marin D, Popat U, Hosing C, Shpall EJ, Kantarjian H, Keating M, Wierda W, Do KA, Largaespada DA, Lee DA, Hackett PB, Champlin RE, Cooper LJ. 
In:
J Clin Invest (2016) 1;126(9): ):3363-76 
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients
Categories:
Transfection 
Authors:
Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang X, Liu B 
In:
Scientific Reports (2016) 6(20070): 10.1038/srep20070 
Type 1 diabetes immunotherapy using polyclonal regulatory T cells.
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Bluestone JA1, Buckner JH2, Fitch M3, Gitelman SE4, Gupta S2, Hellerstein MK3, Herold KC5, Lares A6, Lee MR6, Li K7, Liu W6, Long SA2, Masiello LM6, Nguyen V8, Putnam AL6, Rieck M6, Sayre PH9, Tang Q8. 
In:
Med. Sci. (2015) 7: 315 
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn, Matthew H Porteus 
In:
Nat Biotechnol (2015) 10.1038: nbt.3290 
Rescue of DNA-PK Signaling and T-Cell Differentiation by Targeted Genome Editing in a prkdc Deficient iPSC Disease Model.
Categories:
Transfection 
Authors:
Rahman SH, Kuehle J, Reimann C, Mlambo T, Alzubi J, Maeder ML, Riedel H, Fisch P, Cantz T, Rudolph C, Mussolino C, Joung JK, Schambach A, Cathomen T 
In:
PLoS Genet (2015) 11(5): e1005239 
Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9
Categories:
Transfection 
Authors:
Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS, Stortchevoi A, Bryder D, Musunuru K, Brand H, Tager AM, Allen TM, Talkowski ME, Rossi DJ, Cowan CA 
In:
Cell Stem Cell (2014) 15(5): 643-652 
Genome editing for human gene therapy.
Categories:
Primary Cells and Media, Transfection 
Authors:
Meissner TB1, Mandal PK2, Ferreira LM1, Rossi DJ3, Cowan CA4. 
In:
Methods Enzymol (2014) 546: 273-95 
A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19
Categories:
Primary Cells and Media, Transfection 
Authors:
Singh H Huls H Kebriaei P Cooper LJ 
In:
Immunol Rev (2014) 257(1): 181-190 
A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee JH, Sohn JH, Ryu SY, Hong CS, Moon KD, Park JW 
In:
J Cell Mol Med (2013) 17(10): 1271-81 
Comparison of Lentiviral and Sleeping Beauty Mediated alpha beta T Cell Receptor Gene Transfer
Categories:
Transfection 
Authors:
Anne-Christine Field, Conrad Vink, Richard Gabriel, Roua Al-Subki, Manfred Schmidt, Nicholas Goulden, Hans Stauss, Adrian Thrasher, Emma Morris, Waseem Qasim 
In:
PLoS ONE (2013) 8(6): e68201 
Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells.
Categories:
Transfection 
Authors:
Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, Yang G, Maiti S, Manuri P, Senyukov V, Jena B, Kebriaei P, Champlin RE, Huls H, Cooper LJ. 
In:
PLoS ONE (2013) 8(5): 64138 
Congenital B cell lymphocytosis explained by novel germline CARD11 mutations.
Categories:
Transfection 
Authors:
Snow AL, Xiao W, Stinson JR, Lu W, Chaigne-Delalande B, Zheng L, Pittaluga S, Matthews HF, Schmitz R, Jhavar S, Kuchen S, Kardava L, Wang W, Lamborn IT, Jing H, Raffeld M, Moir S, Fleisher TA, Staudt LM, Su HC, Lenardo MJ. 
In:
J Exp Med (2012) 209(12): 2247-2261 
MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4+ T cells by directly and indirectly targeting DNA methyltransferase 1.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Pan W, Zhu S, Yuan M, Cui H, Wang L, Luo X, Li J, Zhou H, Tang Y, Shen N. 
In:
J Immunol (2010) 184(12): 6773-81 
Optimization of the PiggyBac transposon system for the sustained genetic modification of human T lymphocytes
Categories:
Transfection 
Authors:
Nakazawa Y, Huye LE, Dotti G, Foster AE, Vera JF, Manuri PR, June CH, Rooney CM, Wilson MH. 
In:
J Immunother (2009) 32(8): 826-36 
PRELI is a mitochondrial regulator of human primary T helper cell apoptosis, STAT6 and Th2 cell differentiation
Categories:
Transfection 
Authors:
Tahvanainen J, Kallonen T, Lahteenmaki H, Heiskanen KM, Westermarck J, Rao KV, Lahesmaa R 
In:
Blood (2008) epub: online 
FOXO1 regulates L-Selectin and a network of human T cell homing molecules downstream of phosphatidylinositol 3-kinase
Categories:
Transfection 
Authors:
Fabre S, Carrette F, Chen J, Lang V, Semichon M, Denoyelle C, Lazar V, Cagnard N, Dubart-Kupperschmitt A, Mangeney M, Fruman DA, Bismuth G 
In:
J Immunol (2008) 181(5): 2980-9 
Dual-specificity A-kinase anchoring proteins (AKAPs) contain an additional binding region that enhances targeting of PKA type I
Categories:
Transfection 
Authors:
Jarnaess E, Ruppelt A, Stokka AJ, Lygren B, Scott JD, Tasken K 
In:
J Biol Chem (2008) epub: online 
Protein histidine phosphatase 1 negatively regulates CD4 T cells by inhibiting the K+ channel KCa3.1
Categories:
Transfection 
Authors:
Srivastava S, Zhdanova O, Di L, Li Z, Albaqumi M, Wulff H, Skolnik EY 
In:
Proc Natl Acad Sci USA (2008) 105(38): 14442-6 
Retrovirally induced CTL degranulation mediated by IL-15 expression and infection of mononuclear phagocytes in patients with HTLV-I-associated neurologic disease.
Categories:
Primary Cells and Media 
Authors:
Enose-Akahata Y, Oh U, Grant C, Jacobson S. 
In:
Blood (2008) 112(6): 2400-10 
Modulation of T cell activation by stomatin-like protein 2
Categories:
Transfection 
Authors:
Kirchhof MG, Chau LA, Lemke CD, Vardhana S, Darlington PJ, MÜ¡rquez ME, Taylor R, Rizkalla K, Blanca I, Dustin ML, Madrenas J 
In:
J Immunol (2008) 181(3): 1927-36 
CREB, ATF, and AP-1 transcription factors regulate IFN-gamma secretion by human T cells in response to mycobacterial antigen
Categories:
Transfection 
Authors:
Samten B, Townsend JC, Weis SE, Bhoumik A, Klucar P, Shams H, Barnes PF 
In:
J Immunol (2008) 181(3): 2056-64 
IFN regulatory factor-1 negatively regulates CD4+ CD25+ regulatory T cell differentiation by repressing Foxp3 expression
Categories:
Transfection 
Authors:
Fragale A, Gabriele L, Stellacci E, Borghi P, Perrotti E, Ilari R, Lanciotti A, Remoli AL, Venditti M, Belardelli F, Battistini A 
In:
J Immunol (2008) 181(3): 1673-82 
Acquisition of suppressive function by activated human CD4+ CD25- T cells is associated with the expression of CTLA-4 not FoxP3
Categories:
Transfection 
Authors:
Zheng Y, Manzotti CN, Burke F, Dussably L, Qureshi O, Walker LS, Sansom DM 
In:
J Immunol (2008) 181(3): 1683-91 
ICOS ligation recruits the p50alpha PI3K regulatory subunit to the immunological synapse
Categories:
Transfection 
Authors:
Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, Nunes JA 
In:
J Immunol (2008) 181(3): 1969-77 
Quantitative proteomics reveals GIMAP family proteins 1 and 4 to be differentially regulated during human T helper cell differentiation
Categories:
Transfection 
Authors:
Filen JJ, Filén S, Moulder R, Tuomela S, Ahlfors H, West A, Kouvonen P, Kantola S, Björkman M, Katajamaa M, Rasool O, Nyman TA, Lahesmaa R 
In:
Mol Cell Proteomics (2008) epub: online 
Cell type differences in activity of the Streptomyces bacteriophage phi-C31 integrase
Categories:
Transfection 
Authors:
Maucksch C, Aneja MK, Hennen E, Bohla A, Hoffmann F, Elfinger M, Rosenecker J, Rudolph C 
In:
Nucleic Acids Res (2008) epub: online 
Signaling through ephrin-A ligand leads to activation of Src-family kinases, Akt phosphorylation, and inhibition of antigen receptor-induced apoptosis
Categories:
Transfection 
Authors:
Holen HL, Shadidi M, Narvhus K, Kjܸsnes O, Tierens A, Aasheim HC 
In:
J Leukoc Biol (2008) epub: online 
CXCR4-CCR5: a couple modulating T cell functions
Categories:
Transfection 
Authors:
Contento RL, Molon B, Boularan C, Pozzan T, Manes S, Marullo S, Viola A 
In:
Proc Natl Acad Sci USA (2008) 105(29): 10101-6 
Orphan nuclear receptor NR4A2 expressed in T cells from multiple sclerosis mediates production of inflammatory cytokines
Categories:
Transfection 
Authors:
Doi Y, Oki S, Ozawa T, Hohjoh H, Miyake S, Yamamura T 
In:
Proc Natl Acad Sci USA (2008) 105(24): 8381-6 
SUMOylation Regulates the Transcriptional Activity of JunB in T Lymphocytes
Categories:
Transfection 
Authors:
Garaude J, Farras R, Bossis G, Charni S, Piechaczyk M, Hipskind RA, Villalba M 
In:
J Immunol (2008) 180(9): 5983-90 
The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor
Categories:
Transfection 
Authors:
Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE 
In:
J Immunol (2008) 180(10): 6713-24 
Sleeping Beauty Transposon-mediated Engineering of Human Primary T Cells for Therapy of CD19(+) Lymphoid Malignancies
Categories:
Transfection 
Authors:
Huang X, Guo H, Kang J, Choi S, Zhou TC, Tammana S, Lees CJ, Li ZZ, Milone M, Levine BL, Tolar J, June CH, Scott McIvor R, Wagner JE, Blazar BR, Zhou X 
In:
Mol Ther (2008) 16(3): 580-9 
Transcription factor FOXO3a controls the persistence of memory CD4(+) T cells during HIV infection
Categories:
Transfection 
Authors:
van Grevenynghe J, Procopio FA, He Z, Chomont N, Riou C, Zhang Y, Gimmig S, Boucher G, Wilkinson P, Shi Y, Yassine-Diab B, Said EA, Trautmann L, El Far M, Balderas RS, Boulassel MR, Routy JP, Haddad EK, Sekaly RP 
In:
Nat Med (2008) 14(3): 266-74 
Orai1 and STIM1 move to the immunological synapse and are up-regulated during T cell activation
Categories:
Transfection 
Authors:
Lioudyno MI, Kozak JA, Penna A, Safrina O, Zhang SL, Sen D, Roos J, Stauderman KA, Cahalan MD 
In:
Proc Natl Acad Sci USA (2008) 105(6): 2011-6 
Differential contribution of NFATc2 and NFATc1 to TNF-alpha gene expression in T cells
Categories:
Transfection 
Authors:
Kaminuma O, Kitamura F, Kitamura N, Hiroi T, Miyoshi H, Miyawaki A, Miyatake S 
In:
J Immunol (2008) 180(1): 319-26 
Regulation of human DAP10 gene expression in NK and T cells by Ap-1 transcription factors
Categories:
Transfection 
Authors:
Marusina AI, Burgess SJ, Pathmanathan I, Borrego F, Coligan JE 
In:
J Immunol (2008) 180(1): 409-17 
Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration
Categories:
Transfection 
Authors:
Morin NA, Oakes PW, Hyun YM, Lee D, Chin EY, King MR, Springer TA, Shimaoka M, Tang JX, Reichner JS, Kim M 
In:
J Exp Med (2008) 205(1): 195-205 
4-1BBL Induces TNF Receptor-Associated Factor 1-Dependent Bim Modulation in Human T Cells and Is a Critical Component in the Costimulation-Dependent Rescue of Functionally Impaired HIV-Specific CD8 T Cells
Categories:
Transfection 
Authors:
Wang C, Wen T, Routy JP, Bernard NF, Sekaly RP, Watts TH 
In:
J Immunol (2007) 179(12): 8252-63 
Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin-mediated human T cell adhesion
Categories:
Transfection 
Authors:
Ghandour H, Cullere X, Luscinskas FW, Mayadas TN 
In:
Blood (2007) 110(10): 3682-90 
Cutting Edge: Atypical PKCs Regulate T Lymphocyte Polarity and Scanning Behavior
Categories:
Transfection 
Authors:
Real E, Faure S, Donnadieu E, Delon J 
In:
J Immunol (2007) 179(9): 5649-52 
High-throughput transfection and engineering of primary cells and cultured cell lines - an invaluable tool for research as well as drug development
Categories:
Transfection 
Authors:
Müller-Hartmann H, Faust N, Kazinski M, Kretzschmar T 
In:
Exp Opin Drug Discov (2007) 2(11): 1453-1465 
Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef
Categories:
Transfection 
Authors:
Laforge M, Petit F, Estaquier J, Senik A 
In:
J Virol (2007) 81(20): 11426-40 
Elf-1 Binds to GGAA Elements on the FcR Promoter and Represses Its Expression
Categories:
Transfection 
Authors:
Juang YT, Sumibcay L, Tolnay M, Wang Y, Kyttaris VC, Tsokos GC 
In:
J Immunol (2007) 179(7): 4884-9 
Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase a type I by the a-kinase anchoring protein ezrin
Categories:
Transfection 
Authors:
Ruppelt A, Mosenden R, Grönholm M, Aandahl EM, Tobin D, Carlson CR, Abrahamsen H, Herberg FW, Carpén O, Taskén K 
In:
J Immunol (2007) 179(8): 5159-68 
Impaired T Cell Protein Kinase C{delta} Activation Decreases ERK Pathway Signaling in Idiopathic and Hydralazine-Induced Lupus
Categories:
Transfection 
Authors:
Gorelik G, Fang JY, Wu A, Sawalha AH, Richardson B 
In:
J Immunol (2007) 179(8): 5553-63 
Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function
Categories:
Serum-free and Speciality Media, Transfection 
Authors:
Kubach J, Lutter P, Bopp T, Stoll S, Becker C, Huter E, Richter C, Weingarten P, Warger T, Knop J, Mullner S, Wijdenes J, Schild H, Schmitt E, Jonuleit H 
In:
Blood (2007) 110(5): 1550-8 
ATF2 impairs glucocorticoid receptor-mediated transactivation in human CD8+ T cells
Categories:
Transfection 
Authors:
Li LB, Leung DY, Strand MJ, Goleva E 
In:
Blood (2007) 110(5): 1570-7 
Negative regulation of the SH2-homology-containing protein-tyrosine phosphatase-1 (SHP-1) P2 promoter by the HTLV-1 tax oncoprotein
Categories:
Transfection 
Authors:
Cheng J, Kydd AR, Nakase K, Noonan KM, Murakami A, Tao H, Dwyer M, Xu C, Zhu Q, Marasco WA 
In:
Blood (2007) 110(6): 2110-20 
Interaction between Transmembrane TNF and TNFR1/2 Mediates the Activation of Monocytes by Contact with T Cells
Categories:
Transfection 
Authors:
Rossol M, Meusch U, Pierer M, Kaltenhäuser S, Häntzschel H, Hauschildt S, Wagner U 
In:
J Immunol (2007) 179(6): 4239-48 
CXCL12-induced partitioning of flotillin-1 with lipid rafts plays a role in CXCR4 function
Categories:
Transfection 
Authors:
Giri B, Dixit VD, Ghosh MC, Collins GD, Khan IU, Madara K, Weeraratna AT, Taub DD 
In:
Eur J Immunol (2007) 37(8): 2104-16 
HIV-1 Protease Cleaves Procaspase 8 In Vivo
Categories:
Transfection 
Authors:
Nie Z, Bren GD, Vlahakis SR, Schimnich AA, Brenchley JM, Trushin SA, Warren S, Schnepple DJ, Kovacs CM, Loutfy MR, Douek DC, Badley AD 
In:
J Virol (2007) 81(13): 6947-56 
Cutting Edge: T Cells Respond to Lipopolysaccharide Innately via TLR4 Signaling
Categories:
Transfection 
Authors:
Zanin-Zhorov A, Tal-Lapidot G, Cahalon L, Cohen-Sfady M, Pevsner-Fischer M, Lider O, Cohen IR 
In:
J Immunol (2007) 179(1): 41-4 
Apoptosis-inducing factor regulates death in peripheral T cells
Categories:
Transfection 
Authors:
Srivastava S, Banerjee H, Chaudhry A, Khare A, Sarin A, George A, Bal V, Durdik JM, Rath S 
In:
J Immunol (2007) 179(2): 797-803 
Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression
Categories:
Transfection 
Authors:
Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E 
In:
J Exp Med (2007) 204(6): 1303-1310 
Interaction of PRP4 with Kruppel-Like Factor 13 Regulates CCL5 Transcription
Categories:
Transfection 
Authors:
Huang B, Ahn YT, McPherson L, Clayberger C, Krensky AM 
In:
J Immunol (2007) 178(11): 7081-7 
The Loss of Telomerase Activity in Highly Differentiated CD8+CD28-CD27- T Cells Is Associated with Decreased Akt (Ser473) Phosphorylation
Categories:
Transfection 
Authors:
Plunkett FJ, Franzese O, Finney HM, Fletcher JM, Belaramani LL, Salmon M, Dokal I, Webster D, Lawson AD, Akbar AN 
In:
J Immunol (2007) 178(12): 7710-9 
CD4+ T Cells from Simian Immunodeficiency Virus Disease-Resistant Sooty Mangabeys Produce More IL-2 Than Cells from Disease-Susceptible Species: Involvement of p300 and CREB at the Proximal IL-2 Promoter in IL-2 Up-Regulation
Categories:
Transfection 
Authors:
Bostik P, Noble ES, Stephenson ST, Villinger F, Ansari AA 
In:
J Immunol (2007) 178(12): 7720-9 
Genetic variants of chemokine receptor CCR7 in patients with systemic lupus erythematosus, Sjogren's syndrome and systemic sclerosis
Categories:
Transfection 
Authors:
Kahlmann D, Davalos-Misslitz AC, Ohl L, Stanke F, Witte T, Förster R 
In:
BMC Genet (2007) 8: 33 
Inhibition of BCL11B expression leads to apoptosis of malignant but not normal mature T cells
Categories:
Transfection 
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA 
In:
Oncogene (2007) 26(26): 3797-810 
ADAM10 regulates FasL cell surface expression and modulates FasL-induced cytotoxicity and activation-induced cell death
Categories:
Transfection 
Authors:
Schulte M, Reiss K, Lettau M, Maretzky T, Ludwig A, Hartmann D, de Strooper B, Janssen O, Saftig P 
In:
Cell Death Differ (2007) 14(5): 1040-9 
Epstein-Barr Virus (EBV) Latent Membrane Protein-1 Down-Regulates Tumor Necrosis Factor- (TNF-) Receptor-1 and Confers Resistance to TNF--Induced Apoptosis in T Cells: Implication for the Progression to T-Cell Lymphoma in EBV-Associated Hemophagocytic Syndrome
Categories:
Transfection 
Authors:
Chuang HC, Lay JD, Chuang SE, Hsieh WC, Chang Y, Su IJ 
In:
Am J Pathol (2007) 170(5): 1607-17 
Activation signal transduction by beta1 integrin in T cells from patients with systemic lupus erythematosus
Categories:
Transfection 
Authors:
Nakayamada S, Saito K, Nakano K, Tanaka Y 
In:
Arthritis Rheum (2007) 56(5): 1559-1568 
Up-Regulation of Gene Related to Anergy in Lymphocytes Is Associated with Notch-Mediated Human T Cell Suppression
Categories:
Transfection 
Authors:
Kostianovsky AM, Maier LM, Baecher-Allan C, Anderson AC, Anderson DE 
In:
J Immunol (2007) 178(10): 6158-6163 
CCR7 ligands control basal T cell motility within lymph node slices in a phosphoinositide 3-kinase- independent manner
Categories:
Transfection 
Authors:
Asperti-Boursin F, Real E, Bismuth G, Trautmann A, Donnadieu E 
In:
J Exp Med (2007) 204(5): 1167-79 
NRAS mutation causes a human autoimmune lymphoproliferative syndrome
Categories:
Transfection 
Authors:
Oliveira JB, Bidère N, Niemela JE, Zheng L, Sakai K, Nix CP, Danner RL, Barb J, Munson PJ, Puck JM, Dale J, Straus SE, Fleisher TA, Lenardo MJ 
In:
Proc Natl Acad Sci USA (2007) 104(21): 8953-8958 
Unusual Interplay of Two Types of Ras Activators, RasGRP and SOS, Establishes Sensitive and Robust Ras Activation in Lymphocytes
Categories:
Transfection 
Authors:
Roose JP, Mollenauer M, Ho M, Kurosaki T, Weiss A 
In:
Mol Cell Biol (2007) 27(7): 2732-45 
Extracellular Isoforms of CD6 Generated by Alternative Splicing Regulate Targeting of CD6 to the Immunological Synapse
Categories:
Transfection 
Authors:
Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM 
In:
J Immunol (2007) 178(7): 4351-61 
Human TIM-1 Associates with the TCR Complex and Up-Regulates T Cell Activation Signals
Categories:
Transfection 
Authors:
Binne LL, Scott ML, Rennert PD 
In:
J Immunol (2007) 178(7): 4342-50 
Cutting Edge: Immature Human Dendritic Cells Express Latency-Associated Peptide and Inhibit T Cell Activation in a TGF-beta-Dependent Manner
Categories:
Transfection 
Authors:
Gandhi R, Anderson DE, Weiner HL 
In:
J Immunol (2007) 178(7): 4017-4021 
IFN-alpha-Induced Signal Transduction, Gene Expression, and Antitumor Activity of Immune Effector Cells Are Negatively Regulated by Suppressor of Cytokine Signaling Proteins
Categories:
Transfection 
Authors:
Zimmerer JM, Lesinski GB, Kondadasula SV, Karpa VI, Lehman A, Raychaudhury A, Becknell B, Carson WE 3rd 
In:
J Immunol (2007) 178(8): 4832-45 
Differential Expression and Function of Phosphodiesterase 4 (PDE4) Subtypes in Human Primary CD4+ T Cells: Predominant Role of PDE4D
Categories:
Transfection 
Authors:
Peter D, Jin SL, Conti M, Hatzelmann A, Zitt C 
In:
J Immunol (2007) 178(8): 4820-31 
c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression
Categories:
Transfection 
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM 
In:
Mol Cell Biol (2007) 27(6): 2048-58 
Genome-wide identification of novel genes involved in early Th1 and Th2 cell differentiation
Categories:
Transfection 
Authors:
Lund RJ, Loytomaki M, Naumanen T, Dixon C, Chen Z, Ahlfors H, Tuomela S, Tahvanainen J, Scheinin J, Henttinen T, Rasool O, Lahesmaa R 
In:
J Immunol (2007) 178(6): 3648-60 
Phosphorylated ERM Is Responsible for Increased T Cell Polarization, Adhesion, and Migration in Patients with Systemic Lupus Erythematosus
Categories:
Transfection 
Authors:
Li Y, Harada T, Juang YT, Kyttaris VC, Wang Y, Zidanic M, Tung K, Tsokos GC 
In:
J Immunol (2007) 178(3): 1938-47 
Wnt signaling induces matrix metalloproteinase expression and regulates T cell transmigration
Categories:
Transfection 
Authors:
Wu B, Crampton SP, Hughes CC 
In:
Immunity (2007) 26(2): 227-39 
Dynamic Interplay of Transcriptional Machinery and Chromatin Regulates "Late" Expression of the Chemokine RANTES in T Lymphocytes
Categories:
Transfection 
Authors:
Ahn YT, Huang B, McPherson L, Clayberger C, Krensky AM 
In:
Mol Cell Biol (2007) 27(1): 253-66 
Tumor-induced oxidative stress perturbs nuclear factor-kappaB activity-augmenting tumor necrosis factor-alpha-mediated T-cell death: protection by curcumin
Categories:
Transfection 
Authors:
Bhattacharyya S, Mandal D, Sen GS, Pal S, Banerjee S, Lahiry L, Finke JH, Tannenbaum CS, Das T, Sa G 
In:
Cancer Res (2007) 67(1): 362-70 
Induction of inducible NO synthase in bystander human T cells increases allogeneic responses in the vasculature
Categories:
Transfection 
Authors:
Choy JC, Wang Y, Tellides G, Pober JS 
In:
Proc Natl Acad Sci USA (2007) 104(4): 1313-8 
Cutting Edge: TCR-induced NAB2 enhances T cell function by coactivating IL-2 transcription
Categories:
Transfection 
Authors:
Collins S, Wolfraim LA, Drake CG, Horton MR, Powell JD 
In:
J Immunol (2006) 177(12): 8301-5 
Orchestration of lymphocyte chemotaxis by mitochondrial dynamics
Categories:
Transfection 
Authors:
Campello S, Lacalle RA, Bettella M, Manes S, Scorrano L, Viola A 
In:
J Exp Med (2006) 203(13): 2879-86 
Histidine Phosphorylation of the Potassium Channel KCa3.1 by Nucleoside Diphosphate Kinase B Is Required for Activation of KCa3.1 and CD4 T Cells
Categories:
Transfection 
Authors:
Srivastava S, Li Z, Ko K, Choudhury P, Albaqumi M, Johnson AK, Yan Y, Backer JM, Unutmaz D, Coetzee WA, Skolnik EY 
In:
Mol Cell (2006) 24(5): 665-75 
A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes
Categories:
Transfection 
Authors:
Sheng WY, Chen YR, Wang TC 
In:
FEBS Lett (2006) 580(30): 6819-24 
Measles virus induces expression of SIP110, a constitutively membrane clustered lipid phosphatase, which inhibits T cell proliferation
Categories:
Transfection 
Authors:
Avota E, Harms H, Schneider-Schaulies S 
In:
Cell Microbiol (2006) 8(11): 1826-39 
Human immunodeficiency virus type 1 vpr induces DNA replication stress in vitro and in vivo
Categories:
Transfection 
Authors:
Zimmerman ES, Sherman MP, Blackett JL, Neidleman JA, Kreis C, Mundt P, Williams SA, Warmerdam M, Kahn J, Hecht FM, Grant RM, de Noronha CM, Weyrich AS, Greene WC, Planelles V 
In:
J Virol (2006) 80(21): 10407-18 
CD28 interaction with filamin-A controls lipid raft accumulation at the T-cell immunological synapse
Categories:
Transfection 
Authors:
Tavano R, Contento RL, Baranda SJ, Soligo M, Tuosto L, Manes S, Viola A 
In:
Nat Cell Biol (2006) 8(11): 1270-6 
TGF-beta1 in SP-A preparations influences immune suppressive properties of SP-A on human CD4+ T lymphocytes
Categories:
Transfection 
Authors:
Kunzmann S, Wright JR, Steinhilber W, Kramer BW, Blaser K, Speer CP, Schmidt-Weber C 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 291(4): L747-56 
Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN
Categories:
Transfection 
Authors:
Lowenberg M, Verhaar AP, Bilderbeek J, Marle JV, Buttgereit F, Peppelenbosch MP, van Deventer SJ, Hommes DW 
In:
EMBO Rep (2006) 7(10): 1023-9 
DOCK2 is required for chemokine-promoted human T lymphocyte adhesion under shear stress mediated by the integrin alpha4beta1
Categories:
Transfection 
Authors:
Garcia-Bernal D, Sotillo-Mallo E, Nombela-Arrieta C, Samaniego R, Fukui Y, Stein JV, Teixido J 
In:
J Immunol (2006) 177(8): 5215-25 
Splice variants of human FOXP3 are functional inhibitors of human CD4 T-cell activation
Categories:
Transfection 
Authors:
Smith EL, Finney HM, Nesbitt AM, Ramsdell F, Robinson MK 
In:
Immunology (2006) 119(2): 203-11 
T cell receptor for antigen induces linker for activation of T cell-dependent activation of a negative signaling complex involving Dok-2, SHIP-1, and Grb-2
Categories:
Transfection 
Authors:
Dong S, Corre B, Foulon E, Dufour E, Veillette A, Acuto O, Michel F 
In:
J Exp Med (2006) 203(11): 2509-18 
CTLA-4IG suppresses reactive oxygen species by preventing synovial adherent cell-induced inactivation of Rap1, a Ras family GTPASE mediator of oxidative stress in rheumatoid arthritis T cells
Categories:
Transfection 
Authors:
Remans PH, Wijbrandts CA, Sanders ME, Toes RE, Breedveld FC, Tak PP, van Laar JM, Reedquist KA 
In:
Arthritis Rheum (2006) 54(10): 3135-43 
Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells
Categories:
Transfection 
Authors:
Jones KS, Fugo K, Petrow-Sadowski C, Huang Y, Bertolette DC, Lisinski I, Cushman SW, Jacobson S, Ruscetti FW 
In:
J Virol (2006) 80(17): 8291-302 
Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells
Categories:
Transfection 
Authors:
Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K 
In:
Leukemia (2006) 20(9): 1551-6 
Proprotein convertase furin is preferentially expressed in T helper 1 cells and regulates interferon-
Categories:
Transfection 
Authors:
Pesu M, Muul L, Kanno Y, O'shea JJ 
In:
Blood (2006) 108(3): 983-5 
Lymphocyte Chemotaxis Is Regulated by Histone Deacetylase 6, Independently of Its Deacetylase Activity
Categories:
Transfection 
Authors:
Cabrero JR, Serrador JM, Barreiro O, Mittelbrunn M, Naranjo-Suarez S, Martin-Cofreces N, Vicente-Manzanares M, Mazitschek R, Bradner JE, Avila J, Valenzuela-Fernandez A, Sanchez-Madrid F 
In:
Mol Biol Cell (2006) 17(8): 3435-3445 
Filamin A is required for T cell activation mediated by protein kinase C-theta
Categories:
Transfection 
Authors:
Hayashi K, Altman A 
In:
J Immunol (2006) 177(3): 1721-8 
Autophagy is involved in T cell death after binding of HIV-1 envelope proteins to CXCR4
Categories:
Transfection 
Authors:
Espert L, Denizot M, Grimaldi M, Robert-Hebmann V, Gay B, Varbanov M, Codogno P, Biard-Piechaczyk M 
In:
J Clin Invest (2006) 116(8): 2161-72 
A2A adenosine receptor protects tumors from antitumor T cells
Categories:
Transfection 
Authors:
Ohta A, Gorelik E, Prasad SJ, Ronchese F, Lukashev D, Wong MK, Huang X, Caldwell S, Liu K, Smith P, Chen JF, Jackson EK, Apasov S, Abrams S, Sitkovsky M 
In:
Proc Natl Acad Sci USA (2006) 103(35): 13132-7 
Small Interfering RNA-Mediated Knockdown of Notch Ligands in Primary CD4+ T Cells and Dendritic Cells Enhances Cytokine Production
Categories:
Transfection 
Authors:
Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ 
In:
J Immunol (2006) 177(2): 885-95 
Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7
Categories:
Transfection 
Authors:
Granum S, Sundvold-Gjerstad V, Dai KZ, Kolltveit KM, Hildebrand K, Huitfeldt HS, Lea T, Spurkland A 
In:
BMC Immunol (2006) 7: 15 
Nuclear retention of multiply spliced HIV-1 RNA in resting CD4+ T cells
Categories:
Transfection 
Authors:
Lassen KG, Ramyar KX, Bailey JR, Zhou Y, Siliciano RF 
In:
PLoS Pathog (2006) 2(7): e68 
Dysregulated T cell expression of TIM3 in multiple sclerosis
Categories:
Transfection 
Authors:
Koguchi K, Anderson DE, Yang L, O'connor KC, Kuchroo VK, Hafler DA 
In:
J Exp Med (2006) 203(6): 1413-8 
Protein Kinase C (PKC) and PKC Are the Major PKC Isotypes Involved in TCR Down-Regulation
Categories:
Transfection 
Authors:
von Essen M, Nielsen MW, Bonefeld CM, Boding L, Larsen JM, Leitges M, Baier G, Odum N, Geisler C 
In:
J Immunol (2006) 176(12): 7502-10 
Loss of Siglec expression on T lymphocytes during human evolution
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development
Categories:
Transfection 
Authors:
Gavin MA, Torgerson TR, Houston E, Deroos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY 
In:
Proc Natl Acad Sci USA (2006) 103(17): 6659-64 
Loss of interleukin-2-dependency in HTLV-I-infected T cells on gene silencing of thioredoxin-binding protein-2
Categories:
Transfection 
Authors:
Ahsan MK, Masutani H, Yamaguchi Y, Kim YC, Nosaka K, Matsuoka M, Nishinaka Y, Maeda M, Yodoi J 
In:
Oncogene (2006) 25(15): 2181-91 
Foxp3 represses retroviral transcription by targeting both NF-kappaB and CREB pathways
Categories:
Transfection 
Authors:
Grant C, Oh U, Fugo K, Takenouchi N, Griffith C, Yao K, Newhook TE, Ratner L, Jacobson S 
In:
PLoS Pathog (2006) 2(4): e33 
Modulation of specific surface receptors and activation sensitization in primary resting CD4+ T lymphocytes by the Nef protein of HIV-1
Categories:
Transfection 
Authors:
Keppler OT, Tibroni N, Venzke S, Rauch S and Fackler OT 
In:
J Leukoc Biol (2006) 79(3): 616-27 
Molecular Mechanisms Underlying FOXP3 Induction in Human T Cells
Categories:
Transfection 
Authors:
Mantel PY, Ouaked N, Ruckert B, Karagiannidis C, Welz R, Blaser K, Schmidt-Weber CB 
In:
J Immunol (2006) 176(6): 3593-602 
Lack of Tyrosine 320 Impairs Spontaneous Endocytosis and Enhances Release of HLA-B27 Molecules
Categories:
Transfection 
Authors:
Santos SG, Antoniou AN, Sampaio P, Powis SJ, Arosa FA 
In:
J Immunol (2006) 176(5): 2942-9 
Enrichment of nucleofected primary human CD4(+) T cells: A novel and efficient method for studying gene function and role in human primary T helper cell differentiation
Categories:
Transfection 
Authors:
Tahvanainen J, Pykalainen M, Kallonen T, Lahteenmaki H, Rasool O, Lahesmaa R 
In:
J Immunol Methods (2006) 310(1-2): 30-9 
Phosphatidylinositol 3-kinase functions as a ras effector in the signaling cascade that regulates dephosphorylation of the actin-remodeling protein cofilin after costimulation of untransformed human T lymphocytes
Categories:
Transfection 
Authors:
Wabnitz GH, Nebl G, Klemke M, Schroder AJ, and Samstag Y 
In:
J Immunol (2006) 176(3): 1668-1674 
Suppression of MIP-1beta transcription in human T cells is regulated by inducible cAMP early repressor (ICER)
Categories:
Transfection 
Authors:
Barabitskaja O, Foulke JS Jr, Pati S, Bodor J, Reitz MS Jr 
In:
J Leukoc Biol (2006) 79(2): 378-87 
Cellular Notch responsiveness is defined by phosphoinositide 3-kinase-dependent signals
Categories:
Transfection 
Authors:
McKenzie G, Ward G, Stallwood Y, Briend E, Papadia S, Lennard A, Turner M, Champion B, Hardingham GE 
In:
BMC Cell Biol (2006) 7: 10 
Stable gene transfer and expression in human primary T cells by the Sleeping Beauty transposon system
Categories:
Transfection 
Authors:
Huang X, Wilber AC, Bao L, Tuong D, Tolar J, Orchard PJ, Levine BL, June CH, McIvor RS, Blazar BR and Zhou X 
In:
Blood (2006) 107(2): 483-491 
Early Growth Response-1 Is Required for CD154 Transcription
Categories:
Transfection 
Authors:
Cron RQ, Bandyopadhyay R, Genin A, Brunner M, Kersh GJ, Yin J, Finkel TH and Crow MK 
In:
J Immunol (2006) 176(2): 811-818 
ß2-chimaerin provides a diacylglycerol-dependent mechanism for regulation of adhesion and chemotaxis of T cells
Categories:
Transfection 
Authors:
Siliceo M, Garcia-Bernal D, Carrasco S, Diaz-Flores E, Leskow FC, Teixido J, Kazanietz MG and Merida I 
In:
J Cell Sci (2006) 119(Pt 1): 141-152 
Azathioprine Suppresses Ezrin-Radixin-Moesin-Dependent T Cell-APC Conjugation through Inhibition of Vav Guanosine Exchange Activity on Rac Proteins
Categories:
Transfection 
Authors:
Poppe D, Tiede I, Fritz G, Becker C, Bartsch B, Wirtz S, Strand D, Tanaka S, Galle PR, Bustelo XR and Neurath MF 
In:
J Immunol (2006) 176(1): 640-651 
cIAP2 is a ubiquitin protein ligase for BCL10 and is dysregulated in mucosa-associated lymphoid tissue lymphomas
Categories:
Transfection 
Authors:
Hu S, Du MQ, Park SM, Alcivar A, Qu L, Gupta S, Tang J, Baens M, Ye H, Lee TH, Marynen P, Riley JL and Yang X 
In:
J Clin Invest (2006) 116(1): 174-181 
Constitutive NF-kappaB and NFAT activation in aggressive B-cell lymphomas synergistically activates the CD154 gene and maintains lymphoma cell survival
Categories:
Transfection 
Authors:
Pham LV, Tamayo AT, Yoshimura LC, Lin-Lee YC and Ford RJ 
In:
Blood (2005) 106(12): 3940-3947 
Autoimmune-associated lymphoid tyrosine phosphatase is a gain-of-function variant
Categories:
Transfection 
Authors:
Vang T, Congia M, Macis MD, Musumeci L, Orru V, Zavattari P, Nika K, Tautz L, Tasken K, Cucca F, Mustelin T and Bottini N 
In:
Nat Genet (2005) 37(12): 1317-1319 
Short-Interfering RNA-Mediated Lck Knockdown Results in Augmented Downstream T Cell Responses
Categories:
Transfection 
Authors:
Methi T, Ngai J, Mahic M, Amarzguioui M, Vang T and Tasken K 
In:
J Immunol (2005) 175(11): 7398-7406 
Preligand assembly domain-mediated ligand- independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis
Categories:
Transfection 
Authors:
Clancy L, Mruk K, Archer K, Woelfel M, Mongkolsapaya J, Screaton G, Lenardo MJ and Chan FK 
In:
Proc Natl Acad Sci USA (2005) 102(50): 18099-18104 
Ligation of CD28 by its natural ligand CD86 in the absence of TCR stimulation induces lipid raft polarization in human CD4 T cells
Categories:
Transfection 
Authors:
Kovacs B, Parry RV, Ma Z, Fan E, Shivers DK, Freiberg BA, Thomas AK, Rutherford R, Rumbley CA, Riley JL and Finkel TH 
In:
J Immunol (2005) 175(12): 7848-7854 
The transcription factor GATA-1 potently represses the expression of the HIV-1 co-receptor CCR5 in human T cells and dendritic cells
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
In:
Blood (2005) 106(10): 3440-3448 
Protein phosphatase 2A is a negative regulator of IL-2 production in patients with systemic lupus erythematosus
Categories:
Transfection 
Authors:
Katsiari CG, Kyttaris VC, Juang YT and Tsokos GC 
In:
J Clin Invest (2005) 115(11): 3193-3204 
Interleukin-21 receptor gene induction in human T cells is mediated by T-cell receptor-induced sp1 activity
Categories:
Transfection 
Authors:
Wu Z, Kim HP, Xue HH, Liu H, Zhao K and Leonard WJ 
In:
Mol Cell Biol (2005) 25(22): 9741-9752 
The Cyclic AMP Response Element Modulator Regulates Transcription of the TCR -Chain
Categories:
Transfection 
Authors:
Tenbrock K, Kyttaris VC, Ahlmann M, Ehrchen JM, Tolnay M, Melkonyan H, Mawrin C, Roth J, Sorg C, Juang YT and Tsokos GC 
In:
J Immunol (2005) 175(9): 5975-5980 
Sphingosine Kinase 1 Is a Negative Regulator of CD4+ Th1 Cells
Categories:
Transfection 
Authors:
Yang J, Castle BE, Hanidu A, Stevens L, Yu Y, Li X, Stearns C, Papov V, Rajotte D and Li J 
In:
J Immunol (2005) 175(10): 6580-6588 
Evidence for the Involvement of Tyrosine Kinase ZAP 70 in Nuclear Retinoid Receptor-dependent Transactivation in T Lymphocytes
Categories:
Transfection 
Authors:
Ishaq M, Degray G and Natarajan V 
In:
J Biol Chem (2005) 280(40): 34152-34158 
Negative regulation of CXCR4-mediated chemotaxis by the lipid phosphatase activity of tumor suppressor PTEN
Categories:
Transfection 
Authors:
Gao P, Wange RL, Zhang N, Oppenheim JJ and Howard OM 
In:
Blood (2005) 106(8): 2619-2626 
RNA-based gene therapy for Haemophilia B
Categories:
Transfection 
Authors:
Peppelenbosch MP, Milano F, Ferreira CV, Knapisnka A, Diks SH, Spek A 
In:
Curr Genomics (2005) 6(6): 401-4 
Pharmacologic profiling of transcriptional targets deciphers promoter logic
Categories:
Transfection 
Authors:
Freebern WJ, Haggerty CM, Montano I, McNutt MC, Collins I, Graham A, Chandramouli GV, Stewart DH, Biebuyck HA, Taub DD and Gardner K 
In:
Pharmacogenomics J (2005) 5(5): 305-323 
CTLA-4 up-regulation of lymphocyte function- associated antigen 1 adhesion and clustering as an alternate basis for coreceptor function
Categories:
Transfection 
Authors:
Schneider H, Valk E, da Rocha Dias S, Wei B and Rudd CE 
In:
Proc Natl Acad Sci USA (2005) 102(36): 12861-12866 
Human Immunodeficiency Virus Reactivation by Phorbol Esters or T-Cell Receptor Ligation Requires both PKC and PKC
Categories:
Transfection 
Authors:
Trushin SA, Bren GD, Asin S, Pennington KN, Paya CV and Badley AD 
In:
J Virol (2005) 79(15): 9821-9830 
Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin 41
Categories:
Transfection 
Authors:
Garcia-Bernal D, Wright N, Sotillo-Mallo E, Nombela-Arrieta C, Stein JV, Bustelo XR and Teixido J 
In:
Mol Biol Cell (2005) 16(7): 3223-3235 
Heat shock protein 60 activates cytokine-associated negative regulator suppressor of cytokine signaling 3 in T cells: effects on signaling, chemotaxis, and inflammation
Categories:
Transfection 
Authors:
Zanin-Zhorov A, Tal G, Shivtiel S, Cohen M, Lapidot T, Nussbaum G, Margalit R, Cohen IR and Lider O 
In:
J Immunol (2005) 175(1): 276-285 
TGF- signaling of human T cells is modulated by the ancillary TGF- receptor endoglin
Categories:
Transfection 
Authors:
Schmidt-Weber CB, Letarte M, Kunzmann S, Ruckert B, Bernabeu C and Blaser K 
In:
Int Immunol (2005) 17(7): 921-930 
Defective Vav expression and impaired F-actin reorganization in a subset of patients with common variable immunodeficiency characterized by T-cell defects
Categories:
Transfection 
Authors:
Paccani SR, Boncristiano M, Patrussi L, Ulivieri C, Wack A, Valensin S, Hirst TR, Amedei A, Del Prete G, Telford JL, D'Elios MM and Baldari CT 
In:
Blood (2005) 106(2): 626-634 
Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA
Categories:
Transfection 
Authors:
Mestas J, Crampton SP, Hori T and Hughes CC 
In:
Int Immunol (2005) 17(6): 737-747 
Modulation of specific protein expression levels by PTEN: identification of AKAP121, DHFR, G3BP, Rap1, and RCC1 as potential targets of PTEN
Categories:
Transfection 
Authors:
Huang Y, Wernyj RP, Norton DD, Precht P, Seminario MC and Wange RL 
In:
Oncogene (2005) 24(23): 3819-3829 
T cell costimulation by chemokine receptors
Categories:
Transfection 
Authors:
Molon B, Gri G, Bettella M, Gomez-Mouton C, Lanzavecchia A, Martinez-A C, Manes S and Viola A 
In:
Nat Immunol (2005) 6(5): 465-471 
Lymphocyte arrest requires instantaneous induction of an extended LFA-1 conformation mediated by endothelium-bound chemokines
Categories:
Transfection 
Authors:
Shamri R, Grabovsky V, Gauguet JM, Feigelson S, Manevich E, Kolanus W, Robinson MK, Staunton DE, von Andrian UH and Alon R 
In:
Nat Immunol (2005) 6(5): 497-506 
Cyclic AMP Response Element-Binding Protein Positively Regulates Production of IFN- by T Cells in Response to a Microbial Pathogen
Categories:
Transfection 
Authors:
Samten B, Howard ST, Weis SE, Wu S, Shams H, Townsend JC, Safi H and Barnes PF 
In:
J Immunol (2005) 174(10): 6357-6363 
A novel E3 ubiquitin ligase TRAC-1 positively regulates T cell activation
Categories:
Transfection 
Authors:
Zhao H, Li CC, Pardo J, Chu PC, Liao CX, Huang J, Dong JG, Zhou X, Huang Q, Huang B, Bennett MK, Molineaux SM, Lu H, Daniel-Issakani S, Payan DG and Masuda ES 
In:
J Immunol (2005) 174(9): 5288-5297 
Virus-induced dysfunction of CD4(+)CD25(+) T cells in patients with HTLV-I-associated neuroimmunological disease
Categories:
Transfection 
Authors:
Yamano Y, Takenouchi N, Li HC, Tomaru U, Yao K, Grant CW, Maric DA and Jacobson S 
In:
J Clin Invest (2005) 115(5): 1361-1368 
Cellular APOBEC3G restricts HIV-1 infection in resting CD4+ T cells
Categories:
Transfection 
Authors:
Chiu YL, Soros VB, Kreisberg JF, Stopak K, Yonemoto W and Greene WC 
In:
Nature (2005) 435(7038): 108-114 
Role of GATA-3 in IL-5 gene transcription by CD4+ T cells of asthmatic patients
Categories:
Transfection 
Authors:
Kaminuma O, Mori A, Kitamura N, Hashimoto T, Kitamura F, Inokuma S, Miyatake S 
In:
Int Arch Allergy Immunol (2005) 137 Suppl 1: 55-9 
Stable Activation of Phosphatidylinositol 3-Kinase in the T Cell Immunological Synapse Stimulates Akt Signaling to FoxO1 Nuclear Exclusion and Cell Growth Control
Categories:
Transfection 
Authors:
Fabre S, Lang V, Harriague J, Jobart A, Unterman TG, Trautmann A and Bismuth G 
In:
J Immunol (2005) 174(7): 4161-4171 
Systemic lupus erythematosus serum IgG increases CREM binding to the IL-2 promoter and suppresses IL-2 production through CaMKIV
Categories:
Transfection 
Authors:
Juang YT, Wang Y, Solomou EE, Li Y, Mawrin C, Tenbrock K, Kyttaris VC and Tsokos GC 
In:
J Clin Invest (2005) 115(4): 996-1005 
Kinase-Independent Functions for Itk in TCR-Induced Regulation of Vav and the Actin Cytoskeleton
Categories:
Transfection 
Authors:
Dombroski D, Houghtling RA, Labno CM, Precht P, Takesono A, Caplen NJ, Billadeau DD, Wange RL, Burkhardt JK and Schwartzberg PL 
In:
J Immunol (2005) 174(3): 1385-1392 
Deficient translocation of c-Rel is associated with impaired Th1 cytokine production in T cells from atopic dermatitis patients
Categories:
Transfection 
Authors:
Dieckhoff K, Graf P, Beinhauer B, Schwaerzler C, Carballido JM, Neumann C, Zachmann K and Jung T 
In:
Exp Dermatol (2005) 14(1): 17-25 
IL-2/B7.1 (CD80) fusagene transduction of AML blasts by a self-inactivating lentiviral vector stimulates T cell responses in vitro: a strategy to generate whole cell vaccines for AML.
Categories:
Serum-free and Speciality Media 
Authors:
Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F. 
In:
Mol Ther (2005) 11(1): 120-131 
T-cell Activation Leads to Poor Activation of the HIV-1 Clade E Long Terminal Repeat and Weak Association of Nuclear Factor-B and NFAT with Its Enhancer Region
Categories:
Transfection 
Authors:
Lemieux AM, Pare ME, Audet B, Legault E, Lefort S, Boucher N, Landry S, van Opijnen T, Berkhout B, Naghavi MH, Tremblay MJ and Barbeau B 
In:
J Biol Chem (2004) 279(51): 52949-52960 
CXCR4-Tropic HIV-1 Envelope Glycoprotein Functions as a Viral Chemokine in Unstimulated Primary CD4+ T Lymphocytes
Categories:
Transfection 
Authors:
Balabanian K, Harriague J, Decrion C, Lagane B, Shorte S, Baleux F, Virelizier JL, Arenzana-Seisdedos F and Chakrabarti LA 
In:
J Immunol (2004) 173(12): 7150-7160 
Plant alkaloid tetrandrine downregulates IB kinases-IB-NF-B signaling pathway in human peripheral blood T cell
Categories:
Transfection 
Authors:
Ho LJ, Juan TY, Chao P, Wu WL, Chang DM, Chang SY and Lai JH 
In:
Br J Pharmacol (2004) 143(7): 919-927 
SD-208, a novel transforming growth factor beta receptor I kinase inhibitor, inhibits growth and invasiveness and enhances immunogenicity of murine and human glioma cells in vitro and in vivo
Categories:
Transfection 
Authors:
Uhl M, Aulwurm S, Wischhusen J, Weiler M, Ma JY, Almirez R, Mangadu R, Liu YW, Platten M, Herrlinger U, Murphy A, Wong DH, Wick W, Higgins LS and Weller M 
In:
Cancer Res (2004) 64(21): 7954-7961 
CD28 and Lipid Rafts Coordinate Recruitment of Lck to the Immunological Synapse of Human T Lymphocytes
Categories:
Transfection 
Authors:
Tavano R, Gri G, Molon B, Marinari B, Rudd CE, Tuosto L and Viola A 
In:
J Immunol (2004) 173(9): 5392-5397 
AILIM/ICOS signaling induces T-cell migration/polarization of memory/effector T-cells
Categories:
Transfection 
Authors:
Okamoto N, Nukada Y, Tezuka K, Ohashi K, Mizuno K and Tsuji T 
In:
Int Immunol (2004) 16(10): 1515-1522 
TCR- and CD28-mediated recruitment of phosphodiesterase 4 to lipid rafts potentiates TCR signaling
Categories:
Transfection 
Authors:
Abrahamsen H, Baillie G, Ngai J, Vang T, Nika K, Ruppelt A, Mustelin T, Zaccolo M, Houslay M and Tasken K 
In:
J Immunol (2004) 173(8): 4847-4858 
Rac1 mediates collapse of microvilli on chemokine-activated T lymphocytes
Categories:
Transfection 
Authors:
Nijhara R, van Hennik PB, Gignac ML, Kruhlak MJ, Hordijk PL, Delon J and Shaw S 
In:
J Immunol (2004) 173(8): 4985-4993 
Measles virus interacts with and alters signal transduction in T-cell lipid rafts
Categories:
Transfection 
Authors:
Avota E, Muller N, Klett M and Schneider-Schaulies S 
In:
J Virol (2004) 78(17): 9552-9559 
Tec kinase migrates to the T cell-APC interface independently of ist pleckstrin homology domain
Categories:
Transfection 
Authors:
Garcon F, Bismuth G, Isnardon D, Olive D and Nunes JA 
In:
J Immunol (2004) 173(2): 770-775 
Engagement of ICAM-3 provides a costimulatory signal for human immunodeficiency virus type 1 replication in both activated and quiescent CD4+ T lymphocytes: implications for virus pathogenesis
Categories:
Transfection 
Authors:
Barat C, Gervais P and Tremblay MJ 
In:
J Virol (2004) 78(12): 6692-6697 
Photobleaching-Corrected FRET Efficiency Imaging of Live Cells
Categories:
Transfection 
Authors:
Zal T and Gascoigne NR 
In:
Biophys J (2004) 86(6): 3923-3939 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Cutting Edge: TGF-beta Induces a Regulatory Phenotype in CD4(+)CD25(-) T Cells through Foxp3 Induction and Down-Regulation of Smad7
Categories:
Transfection 
Authors:
Fantini MC, Becker C, Monteleone G, Pallone F, Galle PR and Neurath MF 
In:
J Immunol (2004) 172(9): 5149-5153 
Transcriptional activation of the SH2D2A gene is dependent on a cyclic adenosine 5'-monophosphate-responsive element in the proximal SH2D2A promoter
Categories:
Transfection 
Authors:
Dai KZ, Johansen FE, Kolltveit KM, Aasheim HC, Dembic Z, Vartdal F and Spurkland A 
In:
J Immunol (2004) 172(10): 144-151 
ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation
Categories:
Transfection 
Authors:
Faure S, Salazar-Fontana LI, Semichon M, Tybulewicz VL, Bismuth G, Trautmann A, Germain RN and Delon J 
In:
Nat Immunol (2004) 5(3): 272-279 
GATA-3 in Human T Cell Helper Type 2 Development
Categories:
Transfection 
Authors:
Skapenko A, Leipe J, Niesner U, Devriendt K, Beetz R, Radbruch A, Kalden JR, Lipsky PE and Schulze-Koops H 
In:
J Exp Med (2004) 199(3): 423-428 
Substitution in position 3 of cyclosporin A abolishes the cyclophilin-mediated gain-of-function mechanism but not immunosuppression
Categories:
Transfection 
Authors:
Baumgrass R, Zhang Y, Erdmann F, Thiel A, Weiwad M, Radbruch A and Fischer G 
In:
J Biol Chem (2004) 279(4): 2470-2479 
Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCRzeta chain
Categories:
Transfection 
Authors:
Finney HM, Akbar AN and Lawson ADG 
In:
J Immunol (2004) 172(1): 104-113 
Interleukin 10 regulates cell surface and soluble LIR-2 (CD85d) expression on dendritic cells resulting in T cell hyporesponsiveness in vitro
Categories:
Transfection 
Authors:
Beinhauer BG, McBride JM, Graf P, Pursch E, Bongers M, Rogy M, Korthauer U, de Vries JE, Aversa G and Jung T 
In:
Eur J Immunol (2004) 34: 74-80 
Chemokine stimulation of human periphereal blood T lymphocytes induces rapid dephosphorylation of ERMs which facilitates loss of microvilli and polarization
Categories:
Transfection 
Authors:
Brown MJ, Nijhara R, Hallam JA, Gignac M, Yamada KM, Erlandsen SL, Delon J, Kruhlak M and Shaw S 
In:
Blood (2003) 102(12): 3890-3899 
The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes
Categories:
Transfection 
Authors:
Ganesh L, Burstein E, Guha-Niyogi A, Louder MK, Mascola JR, Klomp LW, Wijmenga C, Duckett CS and Nabel GJ 
In:
Nature (2003) 426: 853-857 
LIME: A new membrane raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling
Categories:
Transfection 
Authors:
Brdickova N, Brdicka T, Angelisova P, Horvath O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B and Horejsi V 
In:
J Exp Med (2003) 198(10): 1453-1462 
CD28 engagement promotes actin polymerization through the activation of the small Rho GTPase Cdc42 in human T cells
Categories:
Transfection 
Authors:
Salazar-Fontana LI, Barr V, Samelson LE and Bierer BE 
In:
J Immunol (2003) 171: 2225-2232 
Targeting combinatorial transcriptional complex assembly at specific modules within the interleukin-2 promoter by immunosuppressant SB203580
Categories:
Transfection 
Authors:
Smith JL, Collins I, Chandramouli GVR, Butscher WG, Zaitseva E, Freebern WJ, Haggerty CM, Doseeva V and Gardner K 
In:
J Biol Chem (2003) 278(42): 41034-41046 
T-cell receptor/CD28 engagement when combined with prostaglandin E2 treatment leads to potent activation of human T-cell leukemia virus type 1
Categories:
Transfection 
Authors:
Dumais N, Pare ME, Mercier S, Bounou S, Marriot SJ, Barbeau B and Tremblay MJ 
In:
J Virol (2003) 77(20): 11170-11179 
Cathepsin D triggers Bax activation, resulting in selective AIF relocation in T lymphocytes entering the early commitment phase to apoptosis
Categories:
Transfection 
Authors:
Bidère N, Lorenzo HK, Carmona S, Laforge M, Harper F, Dumont C and Senik A 
In:
J Biol Chem (2003) 278(33): 31401-31411 
Differential localization and function of ADP-ribosylation factor-6 in anergic human T cells: a potential marker for their identification
Categories:
Transfection 
Authors:
Tzachanis D, Appleman LJ, van Puijenbroek AAFL, Berezovskaya A, Nadler LM and Boussiotis VA 
In:
J Immunol (2003) 171: 1691-1696 
CD154 transcriptional regulation in primary human CD4 T cells
Categories:
Transfection 
Authors:
Cron RQ 
In:
Immunol Res (2003) 27(2-3): 185-202 
Reconstitution of deficient T cell receptor zeta chain restores T cell signaling and 8ments T cell receptor/CD3-induced interleukin-2 production in patients with systemic lupus erythematosus
Categories:
Transfection 
Authors:
Nambiar MP, Fisher CU, Warke VG, Krishnan S, Mitchell JP, Delaney N and Tsokos GC 
In:
Arthritis Rheum (2003) 48(7): 1948-1955 
Histamine enhances TGF-beta1-mediated suppression of TH2 responses
Categories:
Transfection 
Authors:
Kunzmann S, Mantel PY, Wohlfahrt JG, Akdis CA, Blaser K and Schmidt-Weber CB 
In:
FASEB J (2003) 17(9): 1089-1095 
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase A inhibits T cell receptor signaling
Categories:
Transfection 
Authors:
Vang T, Abrahamsen H, Myklebust S, Horejesi V and Taskén K 
In:
J Biol Chem (2003) 278(20): 17597-17600 
Forced expression of the Fc Receptor gamma-chain renders human T cells hyperresponsive to TCR/CD3 stimulation
Categories:
Transfection 
Authors:
Nambiar MP, Fisher CU, Kumar A, Tsokos CG, Warke VG and Tsokos GC 
In:
J Immunol (2003) 170: 2871-2876 
The cyclic adenosine 5' monophosphate response element modulator supresses IL-2 production in stimulated T cells by a chromatin-dependent mechanism
Categories:
Transfection 
Authors:
Tenbrock K, Juang YT, Tolnay M and Tsokos GC 
In:
J Immunol (2003) 170: 2971-2976 
SARA and Hgs attenuate susceptibility to TGF-beta1-mediated T cell supression
Categories:
Transfection 
Authors:
Kunzmann S, Wohlfahrt JG, Itoh S, Asao H, Komada M, Akdis CA, Blaser K and Schmidt-Weber CB 
In:
FASEB J (2003) 17: 194-202 
Memory and flexibility of cytokine gene expression as separable properties of human TH1 and TH2 lymphocytes
Categories:
Transfection 
Authors:
Messi M, Giacchetto I, Nagata K, Lanzavecchia A, Natoli G and Sallusto F 
In:
Nat Immunol (2003) 4: 78-86 
Imaging antigen-induced PI3K activation in T cells
Categories:
Transfection 
Authors:
Harriague J and Bismuth G 
In:
Nat Immunol (2002) 3(11): 1090-1096 
Endothelial cells stimulate T cell NFAT nuclear translocation in the presence of cyclosporin A: involvement of the wnt/glycogen synthase kinase-3beta pathway
Categories:
Transfection 
Authors:
Salazar Murphy LL and Hughes CCW 
In:
J Immunol (2002) 169(7): 3717-3725 
Pleiotropic defects in lymphocyte activation caused by caspase-8 mutations lead to human immunodeficiency
Categories:
Transfection 
Authors:
Chun HJ, Zheng L, Ahmad M, Wang J, Speirs CK, Siegel RM, Dale JK, Puck J, Davis J, Hall CG, Sgota-Smith S, Atkinson TP, Straus SE and Lenardo MJ 
In:
Nature (2002) 419: 395-399 
The phosphatidylinositol phosphatase PTEN is under control of costimulation and regulates proliferation in human T cells
Categories:
Transfection 
Authors:
Schmidt-Weber CB, Wohlfahrt JG, Akdis CA and Blaser K 
In:
Eur J Immunol (2002) 32: 1196-1204 
The immunosuppressant rapamycin represses human immunodeficiency virus type 1 replication
Categories:
Transfection 
Authors:
Roy J, Paquette JS, Fortin JF and Tremblay MJ 
In:
Antimicrob Agents Chemother (2002) 46(11): 3447-3455 
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