Neuron, hippocampal, mouse

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Nerve cells isolated from mouse hippocampus.

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, mouse O-005 4e6 39-77% Plasmid (general) 3 µg 100 µl I/II/2b
AD1 ED-158 8e4 90% 90% Plasmid (general) 18 µg 350 µl 4D Y-Unit

Citations (25)

Methylation in HT22 cells and primary hippocampal neurons with and without isoflurane exposure
Categories:
Primary Cells and Media 
Authors:
Stefanie Klenke , Christian Specking , Maike Stegen , Andrea Engler , Jürgen Peters 
In:
BMC Anesthesiology (2020) 20 (1): 66 
Homozygous SLC6A17 mutations cause autosomal-recessive intellectual disability with progressive tremor, speech impairment, and behavioral problems.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Iqbal Z1, Willemsen MH1, Papon MA2, Musante L3, Benevento M4, Hu H3, Venselaar H5, Wissink-Lindhout WM1, Vulto-van Silfhout AT1, Vissers LE1, de Brouwer AP6, Marouillat S2, Wienker TF3, Ropers HH3, Kahrizi K7, Nadif Kasri N6, Najmabadi H7, Laumonnier F8, Kleefstra T1, van Bokhoven H9. 
In:
Am J Hum Genet (2015) 96(3): 386-396 
Amiodarone biokinetics, the formation of its major oxidative metabolite and neurotoxicity after acute and repeated exposure of brain cell cultures.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pomponio G, Zurich MG, Schultz L, Weiss DG, Romanelli L, Gramowski-Voss A, Di Consiglio E, Testai E. 
In:
Toxicol In Vitro (2015) (15)00013-2: 1 
The kinesin KIF21B participates in the cell surface delivery of ?2 subunit-containing GABAA receptors.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Labonté D, Thies E, Kneussel M. 
In:
Eur J Cell Biol (2014) 93(8-9): 338-346 
The human language and epilepsy associated gene SRPX2 regulates synapse formation and vocalization in mice
Categories:
Transfection 
Authors:
Sia GM, Clem RL, Huganir RL. 
In:
Science (2013) 342: 987-991 
Tuberous sclerosis complex proteins control axon formation
Categories:
Transfection 
Authors:
Choi YJ, Di Nardo A, Kramvis I, Meikle L, Kwiatkowski DJ, Sahin M, He X 
In:
Genes Dev (2008) 22(18): 2485-95 
The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility
Categories:
Transfection 
Authors:
Pandithage R, Lilischkis R, Harting K, Wolf A, Jedamzik B, Lüscher-Firzlaff J, Vervoorts J, Lasonder E, Kremmer E, Knöll B, Lüscher B 
In:
J Cell Biol (2008) 180(5): 915-29 
Flotillin-dependent clustering of the amyloid precursor protein regulates its endocytosis and amyloidogenic processing in neurons
Categories:
Transfection 
Authors:
Schneider A, Rajendran L, Honsho M, Gralle M, Donnert G, Wouters F, Hell SW, Simons M 
In:
J Neurosci (2008) 28(11): 2874-82 
Pyramidal neuron polarity axis is defined at the bipolar stage
Categories:
Transfection 
Authors:
Calderon de Anda F, Gärtner A, Tsai LH, Dotti CG 
In:
J Cell Sci (2008) 121(Pt 2): 178-85 
Cdc42 regulates cofilin during the establishment of neuronal polarity
Categories:
Transfection 
Authors:
Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, Brakebusch C, Bamburg JR, Bradke F 
In:
J Neurosci (2007) 27(48): 13117-29 
High-efficiency transfection of mammalian neurons via nucleofection
Categories:
Transfection 
Authors:
Zeitelhofer M, Vessey JP, Xie Y, Tübing F, Thomas S, Kiebler M, Dahm R 
In:
Nat Protocols (2007) 2(7): 1692-704 
Essential Roles for GSK-3s and GSK-3-Primed Substrates in Neurotrophin-Induced and Hippocampal Axon Growth
Categories:
Transfection 
Authors:
Kim WY, Zhou FQ, Zhou J, Yokota Y, Wang YM, Yoshimura T, Kaibuchi K, Woodgett JR, Anton ES, Snider WD 
In:
Neuron (2006) 52(6): 981-96 
Neuronal polarity is regulated by glycogen synthase kinase-3 (GSK-3) independently of Akt/PKB serine phosphorylation
Categories:
Transfection 
Authors:
Gaertner A, Huang X, Hall A 
In:
J Cell Sci (2006) 119(Pt 19): 3927-34 
Early presynaptic changes during plasticity in cultured hippocampal neurons
Categories:
Transfection 
Authors:
Ninan I, Liu S, Rabinowitz D, Arancio O 
In:
EMBO J (2006) 25(18): 4361-71 
Interneurite affinity is regulated by heterophilic nectin interactions in concert with the cadherin machinery
Categories:
Transfection 
Authors:
Togashi H, Miyoshi J, Honda T, Sakisaka T, Takai Y, Takeichi M 
In:
J Cell Biol (2006) 174(1): 141-51 
Serum response factor controls neuronal circuit assembly in the hippocampus
Categories:
Transfection 
Authors:
Knoell B, Kretz O, Fiedler C, Alberti S, Schutz G, Frotscher M, Nordheim A 
In:
Nat Neurosci (2006) 9(2): 195-204 
Calcium-dependent interaction of Lis1 with IQGAP1 and Cdc42 promotes neuronal motility
Categories:
Transfection 
Authors:
Kholmanskikh SS, Koeller HB, Wynshaw-Boris A, Gomez T, Letourneau PC, Ross ME 
In:
Nat Neurosci (2006) 9(1): 50-7 
Identification of an ectodomain within the LAR protein tyrosine phosphatase receptor that binds homophilically and activates signalling pathways promoting neurite outgrowth
Categories:
Transfection 
Authors:
Yang T, Yin W, Derevyanny VD, Moore LA and Longo FM 
In:
Eur J Neurosci (2005) 22(9): 2159-2170 
Wnt signalling regulates adult hippocampal neurogenesis
Categories:
Transfection 
Authors:
Lie DC, Colamarino SA, Song HJ, Desire L, Mira H, Consiglio A, Lein ES, Jessberger S, Lansford H, Dearie AR, Gage FH 
In:
Nature (2005) 437(7063): 1370-5 
RanBPM is an L1-interacting protein that regulates L1-mediated mitogen-activated protein kinase activation
Categories:
Transfection 
Authors:
Cheng L, Lemmon S and Lemmon V 
In:
J Neurochem (2005) 94(4): 1102-1110 
5-HT7 receptor is coupled to Galpha subunits of heterotrimeric G12-protein to regulate gene transcription and neuronal morphology
Categories:
Transfection 
Authors:
Kvachnina E, Liu G, Dityatev A, Renner U, Dumuis A, Richter DW, Dityateva G, Schachner M, Voyno-Yasenetskaya TA and Ponimaskin EG 
In:
J Neurosci (2005) 25(34): 7821-7830 
Neurite extension in central neurons: a novel role for the receptor tyrosine kinases Ror1 and Ror2
Categories:
Transfection 
Authors:
Paganoni S and Ferreira A 
In:
J Cell Sci (2005) 118(Pt 2): 433-446 
Novel single chain cAMP sensors for receptor-induced signal propagation
Categories:
Transfection 
Authors:
Nikolaev VO, Bunemann M, Hein L, Hannawacker A and Lohse MJ 
In:
J Biol Chem (2004) 279(36): 37215-8 
Differential targeting of the L-type Ca2+ channel alpha 1C (CaV1.2) to synaptic and extrasynaptic compartments in hippocampal neurons
Categories:
Transfection 
Authors:
Obermair GJ, Szabo Z, Bourinet E and Flucher BE 
In:
Eur J Neurosci (2004) 19(8): 2109-2122 
Rapid and efficient electroporation-based gene transfer into primary dissociated neurons
Categories:
Transfection 
Authors:
Dityateva G, Hammond M, Thiel C, Ruonala MO, Delling M, Siebenkotten G, Nix M and Dityatev A 
In:
J Neurosci Methods (2003) 130(1): 65-73 
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