Karpas 299
Export
Human Non-Hodgkin's Ki-positive Large Cell Lymphoma
Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
A-030
5e6
56%
62%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
D-023
2e6
47.2%
60.7%
Plasmid (general)
1 µg
100 µl
I/II/2b
V
D-023
2e6
68.9%
64.8%
Plasmid (general)
1 µg
100 µl
I/II/2b
Citations
CD30-induced signaling is absent in Hodgkin's cells but present in anaplastic large cell lymphoma cells
Categories:
Transfection
Authors:
Hirsch B, Hummel M, Bentink S, Fouladi F, Spang R, Zollinger R, Stein H, Dürkop H
In:
Am J Pathol (2008) 172(2): 510-20
The oncoprotein NPM-ALK of anaplastic large cell lymphoma induces JUNB transcription via ERK1/2 and JunB translation via mTOR signaling
Categories:
Transfection
Authors:
Staber PB, Vesely P, Haq N, Ott RG, Funato K, Bambach I, Fuchs C, Schauer S, Linkesch W, Hrzenjak A, Dirks WG, Sexl V, Bergler H, Kadin ME, Sternberg DW, Kenner L, Hoefler G
In:
Blood (2007) 110(9): 3374-83
Activation of Peroxisome Proliferator-Activated Receptor Contributes to the Survival of T Lymphoma Cells by Affecting Cellular Metabolism
Categories:
Transfection
Authors:
Yang C, Jo SH, Csernus B, Hyjek E, Liu Y, Chadburn A, Wang YL
In:
Am J Pathol (2007) 170(2): 722-32
Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells
Categories:
Transfection
Authors:
Krysov SV, Rowley TF, Al-Shamkhani A
In:
Mol Cancer Ther (2007) 6(2): 703-11
SHP1 tyrosine phosphatase negatively regulates NPM-ALK tyrosine kinase signaling
Categories:
Transfection
Authors:
Honorat JF, Ragab A, Lamant L, Delsol G, Ragab-Thomas J
In:
Blood (2006) 107(10): 4130-8