Vero

normal kidney

Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
monkey
Cell Characteristics:
Adherent

Recommended Media

Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:

  • Vitamins 4X greater than MEM. Vitamins and amino acids greater than BME
  • Types and quantities of amino acids greater than MEM and BME
  • Iron (ferric nitrate)

It is used in a wide range of mammalian cell culture applications.  The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Storage = 2ºC to 8ºC

Liquid:
12-614 = 4.5 g/L glucose, without L-glutamine
12-604 = 4.5 g/L glucose, with L-glutamine
BE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I
12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)
12-917 = 4.5 g/L glucose, without L-glutamine or phenol red
12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine
12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate
12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate

12-707 = 1.0 g/L glucose, without L-glutamine
12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutamine

Powder:
15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate
15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of MDCK and vero cells. It contains HEPES and sodium bicarbonate buffer.
The absence of proteins and only very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.

Some Vero cell strains require additional supplementation with 5.0 µg/L rhEGF for optimal vero cell growth.

Storage = 2°C to 8°C

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V V-001 1e6 77-81% 96-98% Plasmid (general) 2 µg 100 µl I/II/2b
SF DN-100 1e6 89-93% 79% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF DN-100 2e5 92-94% 93% Plasmid (general) 400 ng 20 µl 4D X-Unit

Citations (10)

Categories:
Primary Cells and Media 
Authors:
Kolb AW, Lee K, Larsen I, Craven M, Brandt CR 
In:
PLoS Pathog (2016) 12(3): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miao Q, Santamaria C, Bailey D, Genest J, Ward BJ, Ndao M 
In:
Am J Pathol (2014) 184(4): 976-84 
Categories:
Primary Cells and Media 
Authors:
Wahl-Jensen V, Kurz S, Feldmann F, Buehler LK, Kindrachuk J, DeFilippis V, da Silva Correia J, Früh K, Kuhn JH, Burton DR, Feldmann H. 
In:
Other (2011) 5(10): ePub 
Categories:
Transfection 
Authors:
Konzack S, Thies E, Marx A, Mandelkow EM, Mandelkow E 
In:
J Neurosci (2007) 27(37): 9916-27 
Categories:
Transfection 
Authors:
Sereinig S, Stukova M, Zabolotnyh N, Ferko B, Kittel C, Romanova J, Vinogradova T, Katinger H, Kiselev O, Egorov A 
In:
Clin Vaccine Immunol (2006) 13(8): 898-904 
Categories:
Transfection 
Authors:
Plant EP, Dinman JD 
In:
RNA (2006) 12(4): 666-73 
Categories:
Transfection 
Authors:
Sbalzarini I, Mezzacasa A, Helenius A and Koumoutsakos P 
In:
Biophys J (2005) 89(3): 1482-1492 
Categories:
Transfection 
Authors:
Vonderheit A, Helenius A 
In:
PLoS Biol (2005) 3(7): e233 
Categories:
Transfection 
Authors:
Plant EP, Perez-Alvarado GC, Jacobs JL, Mukhopadhyay B, Hennig M, Dinman JD 
In:
PLoS Biol (2005) 3(6): e172 
Categories:
Transfection 
Authors:
Kittel C, Sereinig S, Ferko B, Stasakova J, Romanova J, Wolkerstorfer A, Katinger H, Egorov A 
In:
Virology (2004) 324(1): 67-73 
+ Show All