CD4+, human

CD4+ T helper cells are white blood cells that are an essential part of the human immune system. CD4+ cells are mad in the spleen, lymph nodes and thumus gland. Mononuclear cells (MNCs or PBMCs) are isolated from apheresis derived peripheral blood and are further purified via immunomagnetic selection directed against CD4.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Transfection Information

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Citations (16)

Categories:
Transfection 
Authors:
Manuel Albanese, Adrian Ruhle, Jennifer Mittermaier, Ernesto Mejías-Pérez, Madeleine Gapp, Andreas Linder, Niklas A Schmacke, Katharina Hofmann, Alexandru A Hennrich, David N Levy, Andreas Humpe, Karl-Klaus Conzelmann, Veit Hornung, Oliver T Fackler, Oliver T Keppler 
In:
Nat Methods (2021) 1: 1-27 
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Thomas R Müller , Sebastian Jarosch 1, Monika Hammel , Justin Leube , Simon Grassmann  , Bettina Bernard 1, Manuel Effenberger , Immanuel Andrä , M Zeeshan Chaudhry , Theresa Käuferle , Antje Malo , Luka Cicin-Sain , Peter Steinberger , Tobias Feuchtinger , Ulrike Protzer , Kathrin Schumann , Michael Neuenhahn , Kilian Schober , Dirk H Busch  
In:
Cell Rep Med (2021) 2(8): 100374 
Categories:
Transfection 
Authors:
Zelda Odé , Jose Condori , Nicolas Peterson , Sheng Zhou , Giedre Krenciute  
In:
Cancers (2020) 12(6): 1704 
Categories:
Transfection 
Authors:
Jeffrey R. Johnson, David C. Crosby, Judd F. Hultquist, Donna Li, John Marlett, Justine Swann, Ruth Hüttenhain, Erik Verschueren, Tasha L. Johnson, Billy W. Newton, Michael Shales,  View ORCID ProfilePedro Beltrao, Alan D. Frankel, Alexander Marson, Oliver I. Fregoso, John A. T. Young, Nevan J. Krogan 
In:
BioResearch Open Access (2020) 1: 1 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A. 
In:
Nat Biotechnol (2019) 1: 1 
Categories:
Primary Cells and Media, Transfection 
Authors:
Schober K, Müller TR, Gökmen F, Grassmann S, Effenberger M, Poltorak M, Stemberger C, Schumann K, Roth TL, Marson A, Busch DH. 
In:
Nat Biomed Eng (2019) 10: 01 
Categories:
Primary Cells and Media, Transfection 
Authors:
Martufi M, Good RB, Rapiteanu R, Schmidt T, Patili E, Tvermosegaard K, New M, Nanthakumar CB, Betts J, Blanchard AD, Maratou K. 
In:
CRISPR J (2019) 2: 31-40 
Categories:
Transfection 
Authors:
Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F. 
In:
Nature (2019) 10(1): 212 
Categories:
Primary Cells and Media, Transfection 
Authors:
Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ. 
In:
Nat Protocols (2019) 14(1): 1-27 
Categories:
Primary Cells and Media, Transfection 
Authors:
Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ.  
In:
Nat Protocols (2019) 14(1): 1-27 
Categories:
Primary Cells and Media, Transfection 
Authors:
Seki A, Rutz S. 
In:
J Exp Med (2018) 215(3): 985-997 
Categories:
Primary Cells and Media 
Authors:
Schultz HS1, Reedtz-Runge SL2, Bäckström BT1, Lamberth K1, Pedersen CR3, Kvarnhammar AM1; ABIRISK consortium. 
In:
PLoS ONE (2017) 12: 5 
Categories:
Transfection 
Authors:
Rupp LJ1, Schumann K, Roybal KT, Gate RE, Ye CJ, Lim WA, Marson A. 
In:
Scientific Reports (2017) 7 (1): 737 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha , Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. 
In:
Nat Genet (2017) 49(2): 193-203 
Categories:
Transfection 
Authors:
Hultquist JF, Schumann K, Woo JM, Manganaro L, McGregor MJ, Doudna J, Simon V, Krogan NJ, Marson A. 
In:
Cell Rep (2016) 17(5): 1438-1452 
Categories:
Primary Cells and Media 
Authors:
Liu G1, Bi Y2, Xue L3, Zhang Y4, Yang H4, Chen X4, Lu Y4, Zhang Z4, Liu H4, Wang X4, Wang R5, Chu Y4, Yang R2. 
In:
Proc Natl Acad Sci USA (2015) 112(9): E957-65 
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