cells

Cardiac fibroblasts isolated from ventricular cardiac tissue

Cell Type:

Fibroblast

Tissue Origin:

heart

Species:

human

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Research Area:

Cancer Research/Cell Biology

Cardiovascular

Cell Characteristics:

Adherent

Recommended Media

Fibroblast Growth Medium-3

FGM-3 is specially formulated for Cardiac Fibroblast growth.
Contains a vial of FBS for a final serum concentration of 10%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
P2	EN-150	1e5	100%	97%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
P2	DS-150	1e5	96%	99%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
P2	CA-137	1e5	96%	99%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit

Citations

Egr-1 upregulates Siva-1 expression and induces cardiac fibroblast apoptosis

Categories:

Primary Cells and Media, Serum-free and Speciality Media

Authors:

Zins K1, Pomyje J2, Hofer E3, Abraham D4, Lucas T5, Aharinejad S6.

In:

Int J Mol Sci (2014) 15(1): 1538-53

Cenderitide-eluting film for potential cardiac patch applications.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Ng XW, Huang Y, Chen HH, Burnett JC Jr, Boey FY, Venkatraman SS.

In:

PLoS ONE (2013) 8(7): e68346

Fibroblast, ventricular cardiac, human

Recommended Media

Transfection Information

Citations