B3Z

T cell hybridoma expressing a TCR that specifically recognizes OVA(257–264) (SIINFEKL) in the context of H-2Kb. The cells carry a beta-galactosidase (lacZ) construct driven by NF-AT elements from the interleukin 2 promoter
Cell Type:
T Cell
Tissue Origin:
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

UltraDOMA Serum-free Hybridoma Medium is a formulation designed for the cultivation of murine, human, and chimeric hybridomas in batch culture and in hollow fiber bioreactors. UltraDOMA™ Medium is supplemented with recombinant human insulin, bovine transferrin and bovine albumin. The total protein concentration is 30 µg/ml.
UltraDOMA ™ Medium does not contain L-glutamine.

Storage = 2ºC to 8ºC

UltraDOMA-PF Protein-free Hydridoma Media are formulations designed for use with hybridoma cell lines of murine, human, and chimeric origin. UltraDOMA-PF Media are completely defined media and do not contain peptides or tissue extracts.
The use of UltraDOMA-PF Media significantly simplifies downstream processing since all proteins present in a given cell culture supernatant are produced by the cells. 

L-glutamine and HEPES buffer are included in the formulation.

Storage = 2ºC  to 8ºC

ProDoma™ Serum-free Hybridoma Media have been developed for cultivation of murine, human, and chimeric hybridomas. ProDoma™ Media are protein-free with a low amount of human recombinant insulin.
All ProDoma™ Media include HEPES as well as sodium bicarbonate in the formulation.

ProDOMA™ 1 is protein-free, non-animal origin, and chemically defined.
ProDOMA™ 3 is a protein-free and non-animal origin product.

ProDOMA™ media contain 0.1% Pluronic® F-68 and doe not contain phenol red or L-glutamine.

Storage = 2°C to 8°C in the dark. Opened bottles should be used rapidly.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L A-030 4e6 71.2% 69.7% Plasmid (general) 1 µg 100 µl I/II/2b
V A-030 4e6 78.4% 68.7% Plasmid (general) 1 µg 100 µl I/II/2b