MDCK

Madin-Darby canine kidney

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
canine
Cell Characteristics:
Adherent

Recommended Media

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

UltraMDCK Serum-free Renal Cell Medium is designed to support the growth of MADIN-DARBY Canine Kidney (MDCK) cells at low and high plating densities.
The medium contains only two proteins: recombinant human insulin and bovine transferrin, yielding a very low protein formulation.

Storage = 2ºC to 8ºC

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of MDCK and vero cells. It contains HEPES and sodium bicarbonate buffer.
The absence of proteins and only very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.

Some Vero cell strains require additional supplementation with 5.0 µg/L rhEGF for optimal vero cell growth.

Storage = 2°C to 8°C

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

ProMDCK™ 2D is a non-animal-origin (NAO) serum free medium that supports the growth of Madin-Darby Canine Kidney Cells (MDCK) in cell culture.  ProMDCK™ 2D medium is optimized for expansion and virus infection of MDCK cells in planar culture (2D)

ProMDCK™ 3D is designed for growth of MDCK cells in 3D culture on microcarrier. ProMDCK™ 3D medium allows direct transition of MDCK cells from ProMDCK™ 2D cultures onto microcarriers

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L A-024 5e5 69-77% 79-87% Plasmid (general) 5 µg 100 µl I/II/2b
SE CA-152 1e6 87-89% 50-64% Plasmid (general) 5 µg 100 µl 4D X-Unit
SE CA-152 2e5 81-85% 41-61% Plasmid (general) 1 µg 20 µl 4D X-Unit
SF FF-150 2e5 90-99% 90-99% Plasmid (general) 200 ng 20 µl Shuttle
SF FF-138 2e5 80-90% 80-90% Plasmid (general) 200 ng 20 µl Shuttle

Citations (20)

Categories:
Transfection 
Authors:
Deborde S, Perret E, Gravotta D, Deora A, Salvarezza S, Schreiner R, Rodriguez-Boulan E 
In:
Nature (2008) 452(7188): 719-23 
Categories:
Transfection 
Authors:
Kanda I, Nishimura N, Nakatsuji H, Yamamura R, Nakanishi H, Sasaki T 
In:
Oncogene (2008) 27(12): 1687-95 
Categories:
Transfection 
Authors:
Sakamoto Y, Ogita H, Komura H, Takai Y 
In:
J Biol Chem (2008) 283(1): 496-505 
Categories:
Transfection 
Authors:
Kim M, Datta A, Brakeman P, Yu W, Mostov KE 
In:
J Cell Sci (2007) 120(Pt 14): 2309-17 
Categories:
Transfection 
Authors:
Storey SM, Gibbons TF, Williams CV, Parr RD, Schroeder F, Ball JM 
In:
J Virol (2007) 81(11): 5472-83 
Categories:
Transfection 
Authors:
Cohen D, Tian Y, Musch A 
In:
Mol Biol Cell (2007) 18(6): 2203-15 
Categories:
Transfection 
Authors:
Ozaki M, Ogita H, Takai Y 
In:
Genes Cells (2007) 12(5): 651-62 
Categories:
Transfection 
Authors:
Stewart GS, King SL, Potter EA, Smith CP 
In:
Am J Physiol Renal Physiol (2007) 292(4): F1157-63 
Categories:
Transfection 
Authors:
Florek M, Bauer N, Janich P, Wilsch-Braeuninger M, Fargeas CA, Marzesco AM, Ehninger G, Thiele C, Huttner WB, Corbeil D 
In:
Cell Tissue Res (2007) 328(1): 31-47 
Categories:
Transfection 
Authors:
Capaldo CT, Macara IG 
In:
Mol Biol Cell (2007) 18(1): 189-200 
Categories:
Transfection 
Authors:
Vieira OV, Gaus K, Verkade P, Fullekrug J, Vaz WL, Simons K 
In:
Proc Natl Acad Sci USA (2006) 103(49): 18556-61 
Categories:
Transfection 
Authors:
Yamada A, Fujita N, Sato T, Okamoto R, Ooshio T, Hirota T, Morimoto K, Irie K, Takai Y 
In:
Oncogene (2006) 25(37): 5085-102 
Categories:
Transfection 
Authors:
Elbert M, Cohen D, Musch A 
In:
Mol Biol Cell (2006) 17(8): 3345-55 
Categories:
Transfection 
Authors:
Potter EA, Stewert G, Smith CP 
In:
Am J Physiol Renal Physiol (2006) 291(1): F122-8 
Categories:
Transfection 
Authors:
McNeil E, Capaldo C and Macara IG 
In:
Mol Biol Cell (2006) 17(4): 1922-32 
Categories:
Transfection 
Authors:
Sato T, Fujita N, Yamada A, Ooshio T, Okamoto R, Irie K and Takai Y 
In:
J Biol Chem (2006) 281(8): 5288-99 
Categories:
Transfection 
Authors:
Chen X, Macara IG 
In:
J Cell Biol (2006) 172(5): 671-8 
Categories:
Transfection 
Authors:
Meder D, Moreno MJ, Verkade P, Vaz WL and Simons K 
In:
Proc Natl Acad Sci USA (2006) 103(2): 329-334 
Categories:
Transfection 
Authors:
Chen X, Macara IG 
In:
Methods Enzymol (2006) 406: 362-74 
Categories:
Transfection 
Authors:
Simons M, Gloy J, Ganner A, Bullerkotte A, Bashkurov M, Kronig C, Schermer B, Benzing T, Cabello OA, Jenny A, Mlodzik M, Polok B, Driever W, Obara T and Walz G 
In:
Nat Genet (2005) 37(5): 537-543 
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