Skeletal Muscle Myoblast, (HSMM) human

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Undifferentiated mononucleated muscle cells that will fuse together to form a multinucleated myotube, then mature into a muscle fibre.

Cell Type:
Myoblast
Tissue Origin:
skeletal muscle
Species:
human
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Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Skeletal Muscle Cell Growth Medium-2

SkGM-2 has been optimized for the proliferation of skeletal muscle myoblasts in a low serum environment.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P5 EY-100 1e6 78% ±1 60% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit
P5 EY-100 2e5 72% ±1 62% ±2 Plasmid (general) 400 ng 20 µl 4D X-Unit

Citations (32)

In vitro exercise model using contractile human and mouse hybrid myotubes
Categories:
Primary Cells and Media 
Authors:
Weijian Chen,1 Mazvita R. Nyasha,1 Masashi Koide,2 Masahiro Tsuchiya,3 Naoki Suzuki,4 Yoshihiro Hagiwara,2 Masashi Aoki,4 and Makoto Kanzaki 
In:
Scientific Reports (2019) 9: 11914 
Regeneration of diaphragm with bio-3D cellular patch
Categories:
Primary Cells and Media 
Authors:
Zhang XY1, Yanagi Y2, Sheng Z3, Nagata K2, Nakayama K4, Taguchi T2 
In:
Biomaterials (2018) 167: 1-14 
Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice.
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Lukjanenko L, Jung MJ, Hegde N, Perruisseau-Carrier C, Migliavacca E, Rozo M, Karaz S, Jacot G, Schmidt M, Li L, Metairon S, Raymond F, Lee U, Sizzano F, Wilson DH, Dumont NA, Palini A, Fässler R, Steiner P, Descombes P, Rudnicki MA, Fan CM, von Maltzahn J, Feige JN, Bentzinger CF 
In:
Nat Med (2016) 22 (8): 897-905 
Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shimo T1, Tachibana K1, Saito K1, Yoshida T2, Tomita E3, Waki R1, Yamamoto T1, Doi T1, Inoue T2, Kawakami J4, Obika S5 
In:
Nucleic Acids Res (2014) 42(12): 8174-87 
Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Araki K1, Kawauchi K, Hirata H, Yamamoto M, Taya Y 
In:
eLife (2013) 2: e01228 
BMS-986001, an HIV nucleoside reverse transcriptase inhibitor, does not degrade mitochondrial DNA in long-term primary cultures of cells isolated from human kidney, muscle, and adipose tissue
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang F1, Flint OP 
In:
Antimicrob Agents Chemother (2013) 57(12): 6205-12 
Simple silicone chamber system for in vitro three-dimensional skeletal muscle tissue formation.
Categories:
Primary Cells and Media 
Authors:
Snyman C1, Goetsch KP1, Myburgh KH2, Niesler CU1 
In:
Other (2013) 28(4): 349 
Characterization of primary human skeletal muscle cells from multiple commercial sources.
Categories:
Primary Cells and Media 
Authors:
Owens J1, Moreira K, Bain G. 
In:
In Vitro Cell Dev Biol Anim. (2013) 49(9): 695-705 
PAX3-FOXO1 induces up-regulation of Noxa sensitizing alveolar rhabdomyosarcoma cells to apoptosis
Categories:
Primary Cells and Media 
Authors:
Marshall AD, Picchione F, Geltink RI, Grosveld GC 
In:
Neoplasia (2013) 15(7): 738-48 
Inhibition of Chikungunya Virus Replication by Harringtonine, a Novel Antiviral That Suppresses Viral Protein Expression
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kaur P, Thiruchelvan M, Lee RC, Chen H, Chen KC, Ng ML, Chu JJ 
In:
Antimicrob Agents Chemother (2013) 57(1): 155-67 
An HMGA2-IGF2BP2 axis regulates myoblast proliferation and myogenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Li Z, Gilbert JA, Zhang Y, Zhang M, Qiu Q, Ramanujan K, Shavlakadze T, Eash JK, Scaramozza A, Goddeeris MM, Kirsch DG, Campbell KP, Brack AS, Glass DJ 
In:
Dev Cell (2012) 23(6): 1176-88 
FGFR4 blockade exerts distinct anti-tumorigenic effects in human embryonal versus alveolar rhabdomyosarcoma
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Crose LE, Etheridge KT, Chen C, Belyea B, Talbot LJ, Bentley RC, Linardic CM. 
In:
Clin Cancer Res (2012) 18(14): 3780-90 
Cockayne syndrome group B protein prevents the accumulation of damaged mitochondria by promoting mitochondrial autophagy
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Scheibye-Knudsen M, Ramamoorthy M, Sykora P, Maynard S, Lin PC, Minor RK, Wilson DM 3rd, Cooper M, Spencer R, de Cabo R, Croteau DL, Bohr VA 
In:
J Exp Med (2012) 209(4): 855-69 
HspB2/Myotonic Dystrophy Protein Kinase Binding Protein (MKBP) as a Novel Molecular Chaperone: Structural and Functional Aspects
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Prabhu S, Raman B, Ramakrishna T, Rao ChM 
In:
PLoS ONE (2012) 7(1): e29810 
Inhibition of the Notch-Hey1 Axis Blocks Embryonal Rhabdomyosarcoma Tumorigenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Belyea BC, Naini S, Bentley RC, Linardic CM 
In:
Clin Cancer Res (2011) 17(23): 7324-36 
Histone methyltransferase KMT1A restrains entry of alveolar rhabdomyosarcoma cells into a myogenic differentiated state.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee MH, Jothi M, Gudkov AV, Mal AK. 
In:
Cancer Res (2011) 71(11): 3921-31 
Aprataxin localizes to mitochondria and preserves mitochondrial function.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sykora P, Croteau DL, Bohr VA, Wilson DM 3rd. 
In:
Proc Natl Acad Sci USA (2011) 108(18): 7437-42 
Pre-microRNA and mature microRNA in human mitochondria
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Barrey E, Saint-Auret G, Bonnamy B, Damas D, Boyer O, Gidrol X 
In:
PLoS ONE (2011) 6(5): e20220 
Mapping and analysis of chromatin state dynamics in nine human cell types.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ernst J, Kheradpour P, Mikkelsen TS, Shoresh N, Ward LD, Epstein CB, Zhang X, Wang L, Issner R, Coyne M, Ku M, Durham T, Kellis M, Bernstein BE 
In:
Nature (2011) 473(7345): 43-9 
Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a dynamic nuclear and sarcomeric protein
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hanel ML, Sun CY, Jones TI, Long SW, Zanotti S, Milner D, Jones PL 
In:
Differentiation (2011) 81(2): 107-18 
Research Resource: The androgen receptor modulates expression of genes with critical roles in muscle development and function
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wyce A, Bai Y, Nagpal S, Thompson CC 
In:
Mol Endocrinol (2010) 24(8): 1665-74 
Promoter DNA Methylation Patterns of Differentiated Cells Are Largely Programmed at the Progenitor Stage
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sørensen AL, Jacobsen BM, Reiner AH, Andersen IS, Collas P 
In:
Mol Biol Cell (2010) 21(12): 2066-77 
Human skeletal muscle drug transporters determine local exposure and toxicity of statins
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Knauer MJ, Urquhart BL, Meyer zu Schwabedissen HE, Schwarz UI, Lemke CJ, Leake BF, Kim RB, Tirona RG 
In:
Circ Res (2010) 106(2): 297-306 
Continuous mild heat stress induces differentiation of mammalian myoblasts, shifting fiber type from fast to slow
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yamaguchi T, Suzuki T, Arai H, Tanabe S, Atomi Y 
In:
Am J Physiol Cell Physiol (2010) 298(1): C140-8 
Chromatin analysis of occluded genes
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee JH, Gaetz J, Bugarija B, Fernandes CJ, Snyder GE, Bush EC, Lahn BT 
In:
Hum Mol Genet (2009) 18(14): 2567-74 
Type 2 deiodinase expression is induced by peroxisomal proliferator-activated receptor-gamma agonists in skeletal myocytes
Categories:
Primary Cells and Media 
Authors:
Grozovsky R, Ribich S, Rosene ML, Mulcahey MA, Huang SA, Patti ME, Bianco AC, Kim BW. 
In:
Endocrinology (2009) 150(4): 1976-83 
Regulation of human myoblast differentiation by PEBP4
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Garcia R, Grindlay J, Rath O, Fee F, Kolch W. 
In:
EMBO Rep (2009) 10(3): 278-84 
Defining the Cooperative Genetic Changes that Temporally Drive Alveolar Rhabdomyosarcoma
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Naini S, Etheridge KT, Adam SJ, Qualman SJ, Bentley RC, Counter CM, Linardic CM 
In:
Cancer Res (2008) 68(23): 9583-8 
Genetic Modeling of Ras-Induced Human Rhabdomyosarcoma
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Linardic CM, Counter CM 
In:
Methods Enzymol (2008) 438: 419-427 
The IGF-I splice variant MGF increases progenitor cells in ALS, dystrophic, and normal muscle.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ates K, Yang SY, Orrell RW, Sinanan AC, Simons P, Solomon A, Beech S, Goldspink G, Lewis MP. 
In:
FEBS Lett (2007) 581(14): 2727-32 
1,1-Bis(3'-indolyl)-1-(p-biphenyl)methane inhibits basal-like breast cancer growth in athymic nude mice
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Su Y, Vanderlaag K, Ireland C, Ortiz J, Grage H, Safe S, Frankel AE. 
In:
Breast Cancer Res (2007) 9(4): R56 
TWEAK, via its receptor Fn14, is a novel regulator of mesenchymal progenitor cells and skeletal muscle regeneration
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Girgenrath M, Weng S, Kostek CA, Browning B, Wang M, Brown SA, Winkles JA, Michaelson JS, Allaire N, Schneider P, Scott ML, Hsu YM, Yagita H, Flavell RA, Miller JB, Burkly LC, Zheng TS 
In:
EMBO J (2006) 25(24): 5826-39 
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