Endothelial, pulmonary artery (HPAEC), human

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Large vessel endothelial cells are isolated from the pulmonary artery.

Cell Type:
Pulmonary artery endo.
Tissue Origin:
artery
Species:
human
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Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Endothelial Cell Growth Medium 2

Endothelial cell media are low serum media optimized for the proliferation of endothelial cells.

EGM-2 is a refined version of EGM.
 - Does not contain BBE
 - Final serum concentration is 2%
 - Improved cell proliferation over EGM

EGM-2 can be used to grow all of Clonetics Endothelial Cells except microvascular, coronary artery, umbilical artery, and iliac artery

Endothelial Cell Growth Medium - Microvascular

This medium has been discontinued end of 2023. As alternative we recommend the EGM-2 MV CC-3202

Developed for microvascular and coronary artery endothelial cells
Same basal medium as in EGM
Final serum concentration is 5%
EGM-MV can be used to grow most Clonetics Endothelial Cells

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
AD1 CA-215 5e4 60-80% good Plasmid (general) 16 µg 350 µl 4D Y-Unit

Citations (40)

Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts.
Categories:
Primary Cells and Media, 3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bastijanic JM, Kligman FL, Marchant RE, Kottke-Marchant K 
In:
J Biomed Mater Res A (2016) 104(1): 71-81 
Recombinant human interferon alpha 2b prevents and reverses experimental pulmonary hypertension.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bauer EM, Zheng H, Lotze MT, Bauer PM. 
In:
PLoS ONE (2014) 9(5): e96720 
Gene Expression Profiles of Human Dendritic Cells Interacting with Aspergillus fumigatus in a Bilayer Model of the Alveolar Epithelium/Endothelium Interface.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morton CO, Fliesser M, Dittrich M, Mueller T, Bauer R, Kneitz S, Hope W, Rogers TR, Einsele H, Loeffler J. 
In:
PLoS Med (2014) 9(5): e98279 
GRP78 is a novel receptor initiating a vascular barrier protective response to oxidized phospholipids.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Birukova AA, Singleton PA, Gawlak G, Tian X, Mirzapoiazova T, Mambetsariev B, Dubrovskyi O, Oskolkova OV, Bochkov VN, Birukov KG. 
In:
Mol Biol Cell (2014) mbc.E13: 12-0743 
Stiffness-Activated GEF-H1 Expression Exacerbates LPS-Induced Lung Inflammation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mambetsariev I, Tian Y, Wu T, Lavoie T, Solway J, Birukov KG, Birukova AA. 
In:
PLoS ONE (2014) 9(4): e92670 
Control of vascular permeability by atrial natriuretic peptide via a GEF-H1-dependent mechanism.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tian X, Tian Y, Gawlak G, Sarich N, Wu T, Birukova AA. 
In:
J Biol Chem (2014) 289(8): 5168-83 
Tumor stiffness is unrelated to myosin light chain phosphorylation in cancer cells
Categories:
Primary Cells and Media 
Authors:
Yu HJ1, Serebryannyy LA, Fry M, Greene M, Chernaya O, Hu WY, Chew TL, Mahmud N, Kadkol SS, Glover S, Prins G, Strakova Z, de Lanerolle P 
In:
PLoS ONE (2013) 8(11): e79776 
Hypoxia mediates mutual repression between microRNA-27a and PPAR? in the pulmonary vasculature.
Categories:
Primary Cells and Media 
Authors:
Kang BY, Park KK, Green DE, Bijli KM, Searles CD, Sutliff RL, Hart CM. 
In:
PLoS ONE (2013) 8(11): e79503 
Mechanisms of haptoglobin protection against hemoglobin peroxidation triggered endothelial damage
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schaer CA, Deuel JW, Bittermann AG, Rubio IG, Schoedon G, Spahn DR, Wepf RA, Vallelian F, Schaer DJ. 
In:
Cell Death Differ (2013) 20(11): 1569-79 
Inhibition of IGF-1R prevents ionizing radiation-induced primary endothelial cell senescence.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Panganiban RA, Day RM. 
In:
PLoS ONE (2013) 8(10): e78589. 
Rap-afadin axis in control of Rho signaling and endothelial barrier recovery.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Birukova AA, Tian X, Tian Y, Higginbotham K, Birukov KG. 
In:
Mol Biol Cell (2013) 24(17): 2678-88 
Serum can overcome contact inhibition in confluent human pulmonary artery smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Solodushko V, Khader HA, Fouty BW 
In:
PLoS ONE (2013) 8(8): e71490 
Ezrin/radixin/moesin proteins differentially regulate endothelial hyperpermeability after thrombin.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Adyshev DM, Dudek SM, Moldobaeva N, Kim KM, Ma SF, Kasa A, Garcia JG, Verin AD. 
In:
Am J Physiol Lung Cell Mol Physiol (2013) 305(3): L240-55 
Double-stranded RNA attenuates the barrier function of human pulmonary artery endothelial cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bálint Z, Zabini D, Konya V, Nagaraj C, Végh AG, Váró G, Wilhelm I, Fazakas C, Krizbai IA, Heinemann A, Olschewski H, Olschewski A. 
In:
PLoS ONE (2013) 8(6): e63776 
Endothelial GATA-6 deficiency promotes pulmonary arterial hypertension.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ghatnekar A, Chrobak I, Reese C, Stawski L, Seta F, Wirrig E, Paez-Cortez J, Markiewicz M, Asano Y, Harley R, Silver R, Feghali-Bostwick C, Trojanowska M. 
In:
Am J Pathol (2013) 182(6): 2391-406 
Thrombin has biphasic effects on the nitric oxide-cGMP pathway in endothelial cells and contributes to experimental pulmonary hypertension.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nickel KF, Laux V, Heumann R, von Degenfeld G. 
In:
PLoS ONE (2013) 8(6): e63504 
Coronin 1B regulates S1P-induced human lung endothelial cell chemotaxis: role of PLD2, protein kinase C and Rac1 signal transduction
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Usatyuk PV, Burns M, Mohan V, Pendyala S, He D, Ebenezer DL, Harijith A, Fu P, Huang LS, Bear JE, Garcia JG, Natarajan V. 
In:
PLoS ONE (2013) 8(5): e63007 
Acidosis activation of the proton-sensing GPR4 receptor stimulates vascular endothelial cell inflammatory responses revealed by transcriptome analysis.
Categories:
Primary Cells and Media 
Authors:
Dong L, Li Z, Leffler NR, Asch AS, Chi JT, Yang LV. 
In:
PLoS ONE (2013) 8(4): e61991 
Measurement of local permeability at subcellular level in cell models of agonist- and ventilator-induced lung injury.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Dubrovskyi O, Birukova AA, Birukov KG. 
In:
Kidney Int (2013) 93(2): 254-63 
Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rajput C, Kini V, Smith M, Yazbeck P, Chavez A, Schmidt T, Zhang W, Knezevic N, Komarova Y, Mehta D. 
In:
J Biol Chem (2013) 288(6): 4241-50 
RACK1 is involved in endothelial barrier regulation via its two novel interacting partners.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Boratkó A, Gergely P, Csortos C. 
In:
Other (2013) 11(1): 2 
Critical role of non-muscle myosin light chain kinase in thrombin-induced endothelial cell inflammation and lung PMN infiltration
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fazal F, Bijli KM, Murrill M, Leonard A, Minhajuddin M, Anwar KN, Finkelstein JN, Watterson DM, Rahman A. 
In:
PLoS ONE (2013) 8(3): e59965 
Mitochondrial DAMPs increase endothelial permeability through neutrophil dependent and independent pathways.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sun S, Sursal T, Adibnia Y, Zhao C, Zheng Y, Li H, Otterbein LE, Hauser CJ, Itagaki K. 
In:
PLoS ONE (2013) 8(3): e59989 
PPARd agonist GW501516 inhibits PDGF-stimulated pulmonary arterial smooth muscle cell function related to pathological vascular remodeling
Categories:
Primary Cells and Media 
Authors:
Liu G, Li X, Li Y, Tang X, Xu J, Li R, Hao P, Sun Y. 
In:
Biomed Res Int. (2013) 2013: 903947 
High mobility group Box 1 inhibits human pulmonary artery endothelial cell migration via a Toll-like receptor 4- and interferon response factor 3-dependent mechanism(s).
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bauer EM, Shapiro R, Billiar TR, Bauer PM. 
In:
J Cell Biochem (2013) 288(2): 1365-73 
Critical role of S1PR1 and integrin ß4 in HGF/c-Met-mediated increases in vascular integrity.
Categories:
 
Authors:
Ephstein Y, Singleton PA, Chen W, Wang L, Salgia R, Kanteti P, Dudek SM, Garcia JG, Jacobson JR. 
In:
J Biol Chem (2013) 288(4): 2191-200 
Iloprost improves endothelial barrier function in lipopolysaccharide-induced lung injury.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Birukova AA1, Wu T, Tian Y, Meliton A, Sarich N, Tian X, Leff A, Birukov KG. 
In:
Eur Respir J (2013) 41(1): 165-76 
Combination of voriconazole and anidulafungin for treatment of triazole-resistant aspergillus fumigatus in an in vitro model of invasive pulmonary aspergillosis
Categories:
Primary Cells and Media 
Authors:
Jeans AR, Howard SJ, Al-Nakeeb Z, Goodwin J, Gregson L, Warn PA, Hope WW. 
In:
Antimicrob Agents Chemother (2012) 56(10): 5180-5 
A role for VEGFR2 activation in endothelial responses caused by barrier disruptive OxPAPC concentrations.
Categories:
Primary Cells and Media 
Authors:
Birukova AA, Lee S, Starosta V, Wu T, Ho T, Kim J, Berliner JA, Birukov KG. 
In:
PLoS ONE (2012) 1: e30957 
BMP-9 induced endothelial cell tubule formation and inhibition of migration involves Smad1 driven endothelin-1 production
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Park JE, Shao D, Upton PD, Desouza P, Adcock IM, Davies RJ, Morrell NW, Griffiths MJ, Wort SJ. 
In:
PLoS ONE (2012) 7(1): e30075 
Altered MicroRNA processing in heritable pulmonary arterial hypertension: an important role for Smad-8
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drake KM, Zygmunt D, Mavrakis L, Harbor P, Wang L, Comhair SA, Erzurum SC, Aldred MA 
In:
Am J Respir Crit Care Med (2011) 184(12): 1400-8 
Somatic chromosome abnormalities in the lungs of patients with pulmonary arterial hypertension
Categories:
Primary Cells and Media 
Authors:
Aldred MA, Comhair SA, Varella-Garcia M, Asosingh K, Xu W, Noon GP, Thistlethwaite PA, Tuder RM, Erzurum SC, Geraci MW, Coldren CD 
In:
Am J Respir Crit Care Med (2010) 182(9): 1153-60 
Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool
Categories:
Primary Cells and Media 
Authors:
Shahani T, Lavend\'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. 
In:
Blood (2010) 115(23): 4902-9 
Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD).
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Patel N, Sundaram N, Yang M, Madigan C, Kalra VK, Malik P. 
In:
J Biol Chem (2010) 285(22): 16713-22 
Rap1 and its effector KRIT1/CCM1 regulate beta-catenin signaling.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Glading AJ, Ginsberg MH. 
In:
Dis Model Mech (2010) 3(1-2): 73-83 
T-cadherin is located in the nucleus and centrosomes in endothelial cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Andreeva AV, Kutuzov MA, Tkachuk VA, Voyno-Yasenetskaya TA. 
In:
Am J Physiol Cell Physiol (2009) 297(5): C1168-77 
MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan SY, Zhang YY, Hemann C, Mahoney CE, Zweier JL, Loscalzo J 
In:
Cell Metab (2009) 10(4): 273-84 
Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hao Q, Wang L, Zhao ZJ, Tang H. 
In:
J Biol Chem (2009) 284(2): 799-806 
Paxillin is involved in the differential regulation of endothelial barrier by HGF and VEGF
Categories:
Primary Cells and Media 
Authors:
Birukova AA, Cokic I, Moldobaeva N, Birukov KG. 
In:
Am J Respir Cell Mol Biol (2009) 40(1): 99-107 
Endothelial cell diversity revealed by global expression profiling
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8 
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