CHO-Flp-In

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic

Cell Type:
Ovary Epith.
Tissue Origin:
Species:
hamster
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Developed for low density (clonal) growth of CHO cells.

Storage = 2ºC to 8ºC

12-618 = with L-glutamine
BE02-014 = with UltraGlutamine I, without thymidine

Ham's F12 Medium is a nutrient mixture designed to cultivate a wide variety of mammalian and hybridoma cells (including low density (clonal) growth of CHO cells) when used with serum in combination with hormones and transferrin.

ProCHO™ Protein-free CHO Media were developed specifically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These protein-free formulations support high-density cultures without the need for animal derived components.
Very low levels of recombinant insulin facilitate both downstream purification and regulatory compliance.

The following media systems are available:
- ProCHO™ 4 Medium – For concurrent transition of adherent CHO cells to serum-free and suspension culture; supports faster doubling times
- ProCHO™ 5 Medium – For CHO cells already growing in suspension; supports increased protein production
- ProCHO™ AT Medium – For adherent culture of CHO cells

Storage = 2°C to 8°C

12-029Q - ProCHO™ 4 with phenol red and 0.1% Pluronic® F-68; but without L-glutamine, hypoxanthine, or thymidine.
04-919Q - ProCHO™ 4 with 0.1% Pluronic® F-68; and without phenol red, L-glutamine, hypoxanthine, or thymidine.

12-766Q - ProCHO™ 5  with 0.1% Pluronic® F-68; and without L-glutamine, phenol red, hypoxanthine, or thymidine.
BE15-766 - ProCHO™ 5 powder without phenol red

BE02-016 - ProCHO™-AT contains L-glutamine and does not contain hypoxanthine or thymidine.

PowerCHO™ Chemically Defined, Serum-free and hydrolysate-free CHO Media are non-animal origin media designed to support the growth of CHO cell lines, particularly high-density suspension cultures.

For therapeutic bioprocessing applications, these protein-free formulations also facilitate both downstream purification and regulatory compliance.

Storage = 2°C to 8°C

12-770Q - PowerCHO™-1 (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)
12-771Q - PowerCHO™-2 (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)
BE15-771N - PowerCHO™-2 powder (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)
12-772Q - PowerCHO™-3 (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)

BE12-776Q - PowerCHO™-GS (with HEPES and Pluronic® F-68, without insulin, phenol red or L-glutamine)
PowerCHO™ -GS is designed for use with Lonza's Proprietary GS Gene Expression System™.

12-929Q - PowerCHO™ - Advance

ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS), which is particularly advantageous for freezing of cells cultured in a serum-free and animal component-free environment.
This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.

ProFreeze™ Medium requires the addition of 15% reagent or spectrophotometric grade dimethylsulfoxide (DMSO) at time of use.

Storage = 2°C to 8°C

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.