Epithelial, Small Airway, human (SAEC)

Human small airway epithelial cells isolated from the distal portion of the lung in the 1 mm bronchiole area

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Airway cell media are serum-free media that have been optimized for the proliferation of airway cells.
The SAGM BulletKit was specifically developed to provide superior growth for Human Small Airway Epithelial Cells

The medium is designed specifically for culturing small airway epithelial cells in an air-liquid interface system.

The S-ALI™ BulletKit™ contains:
- S-ALI™ Growth Basal Media
- S-ALI™ Differentiation Basal Medium
- S-ALI™SingleQuots™ kit

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic T-013 5e5 47% ±14 96% ±17 Plasmid (general) 2 µg 100 µl I/II/2b
Epithelial, basic T-020 5e5 46% ±13 96% ±25 Plasmid (general) 2 µg 100 µl I/II/2b
Epithelial, basic T-023 5e5 47% ±14 99% ±24 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (59)

Categories:
Primary Cells and Media, Transfection 
Authors:
Dickson A, Mullooly N, Serrano A, Escudero-Ibarz L, Wiggins C, Gianni D 
In:
SLAS Discov (2023) 2: 29-35 
Categories:
Primary Cells and Media 
Authors:
Anne Sturrock, Elizabeth Zimmerman, My Helms, Theodore G Liou, Robert Paine 3rd 
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Physiol Rep (2021) 9: e14854 
Categories:
Primary Cells and Media 
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In:
Scientific Reports (2017) 7(1): 11644 
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Chandorkar P, Posch W, Zaderer V, Blatzer M, Steger M, Ammann CG, Binder U, Hermann M, Hörtnagl P, Lass-Flörl C, Wilflingseder D 
In:
Scientific Reports (2017) 7(1): 10.1038/s41598-017-11271-4 
Categories:
Primary Cells and Media 
Authors:
Carpenter PM, Sivadas P, Hua SS, Xiao C, Gutierrez AB, Ngo T, Gershon PD 
In:
Cancer Medicine (2017) 6(1): 220-234 
Categories:
Primary Cells and Media 
Authors:
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Primary Cells and Media, 3D Cell Culture  
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PLoS ONE (2015) 10: 6 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Am J Respir Cell Mol Biol (2014) 50(3): 559-70 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Particle and Fibre Toxicology (2014) 11(6): 1-15 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS ONE (2013) 8(12): e82620 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Toxicol Appl Pharmacol (2013) 272(2): 476-89 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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PLoS Pathog (2013) 9(6): e1003407 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Scientific Reports (2013) 3: 1911 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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British Journal of Anaesthsia (2013) 110(4):: 637-45 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS ONE (2013) 8(3): e57393 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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In:
Am J Physiol Lung Cell Mol Physiol (2013) 304(6): 415-27 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Respir Res (2013) 14: 9 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ivarsson M, Schollin J, Björkqvist M 
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Acta Paediatr. (2012) 102(10): 1010-6 
Categories:
Primary Cells and Media, Bioassay  
Authors:
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PLoS ONE (2012) Identificati: 7(8) 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media 
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