Epithelial, Small Airway, human (SAEC)

Human small airway epithelial cells isolated from the distal portion of the lung in the 1 mm bronchiole area

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Airway cell media are serum-free media that have been optimized for the proliferation of airway cells.
The SAGM BulletKit was specifically developed to provide superior growth for Human Small Airway Epithelial Cells

The medium is designed specifically for culturing small airway epithelial cells in an air-liquid interface system.

The S-ALI™ BulletKit™ contains:
- S-ALI™ Growth Basal Media
- S-ALI™ Differentiation Basal Medium
- S-ALI™SingleQuots™ kit

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic T-013 5e5 47% ±14 96% ±17 Plasmid (general) 2 µg 100 µl I/II/2b
Epithelial, basic T-020 5e5 46% ±13 96% ±25 Plasmid (general) 2 µg 100 µl I/II/2b
Epithelial, basic T-023 5e5 47% ±14 99% ±24 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (55)

Categories:
Primary Cells and Media, 3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chandorkar P, Posch W, Zaderer V, Blatzer M, Steger M, Ammann CG, Binder U, Hermann M, Hörtnagl P, Lass-Flörl C, Wilflingseder D 
In:
Scientific Reports (2017) 7(1): 10.1038/s41598-017-11271-4 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Carpenter PM, Sivadas P, Hua SS, Xiao C, Gutierrez AB, Ngo T, Gershon PD 
In:
Cancer Medicine (2017) 6(1): 220-234 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Scheraga RG, Thompson C, Tulapurkar ME, Nagarsekar AC, Cowan M, Potla R, Sun J, Cai R, Logun C, Shelhamer J, Todd NW, Singh IS, Luzina IG, Atamas SP, Hasday JD 
In:
Am J Physiol Lung Cell Mol Physiol (2016) 311(5): L941-L955 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Daidoji T, Watanabe Y, Ibrahim MS, Yasugi M, Maruyama H, Masuda T, Arai F, Ohba T, Honda A, Ikuta K, Nakaya T. 
In:
J Biol Chem (2015) unknown: unknown 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Furukawa M, Wheeler S, Clark AM, Wells A 
In:
PLoS ONE (2015) 10 (1): e0118060 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Khabibullin D, Medvetz DA, Pinilla M, Hariharan V, Li C, Hergrueter A, Laucho Contreras M, Zhang E, Parkhitko A, Yu JJ, Owen CA, Huang H, Baron RM, Henske EP 
In:
Respir Res (2014) 2(8): e12107 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Heyl KA, Klassert TE, Heinrich A, Müller MM, Klaile E, Dienemann H, Grünewald C, Bals R, Singer BB, Slevogt H 
In:
mBio (2014) 5(5): e01492-14 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Geraghty P, Hardigan A, Foronjy RF 
In:
Am J Respir Cell Mol Biol (2014) 50(3): 559-70 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Siegrist KJ, Reynolds SH, Kashon ML, Lowry DT, Dong C, Hubbs AF, Young SH, Salisbury JL, Porter DW, Benkovic SA, McCawley M, Keane MJ, Mastovich JT, Bunker KL, Cena LG, Sparrow MC, Sturgeon JL, Dinu CZ1, Sargent LM 
In:
Particle and Fibre Toxicology (2014) 11(6): 1-15 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kwon M, Lee SJ, Reddy S, Rybak Y, Adem A, Libutti SK 
In:
PLoS ONE (2013) 8(12): e82620 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Snyder-Talkington BN1, Dymacek J, Porter DW, Wolfarth MG, Mercer RR, Pacurari M, Denvir J, Castranova V, Qian Y, Guo NL 
In:
Toxicol Appl Pharmacol (2013) 272(2): 476-89 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Roberts JK1, Moore CD1, Romero EG1, Ward RM2, Yost GS1, Reilly CA1. 
In:
Other (2013) 2: 173 
Categories:
 
Authors:
Verway M, Bouttier M, Wang TT, Carrier M, Calderon M, An BS, Devemy E, McIntosh F, Divangahi M, Behr MA, White JH. 
In:
PLoS Pathog (2013) 9(6): e1003407 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Petrache I, Kamocki K, Poirier C, Pewzner-Jung Y, Laviad EL, Schweitzer KS, Van Demark M, Justice MJ, Hubbard WC, Futerman AH. 
In:
PLoS ONE (2013) 8(5): e62968 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Petrache I, Kamocki K, Poirier C, Pewzner-Jung Y, Laviad EL, Schweitzer KS, Van Demark M, Justice MJ, Hubbard WC, Futerman AH 
In:
PLoS ONE (2013) 8(5): e62968 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sato T, Kaneda A, Tsuji S, Isagawa T, Yamamoto S, Fujita T, Yamanaka R, Tanaka Y, Nukiwa T, Marquez VE, Ishikawa Y, Ichinose M, Aburatani H 
In:
Scientific Reports (2013) 3: 1911 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Watanabe K, Iwahara C, Nakayama H, Iwabuchi K, Matsukawa T, Yokoyama K, Yamaguchi K, Kamiyama Y, Inada E 
In:
British Journal of Anaesthsia (2013) 110(4):: 637-45 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bartis D, Csongei V, Weich A, Kiss E, Barko S, Kovacs T, Avdicevic M, D\\\\\\\'Souza VK, Rapp J, Kvell K, Jakab L, Nyitrai M, Molnar TF, Thickett DR, Laszlo T, Pongracz JE 
In:
PLoS ONE (2013) 8(3): e57393 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zemans RL1, McClendon J, Aschner Y, Briones N, Young SK, Lau LF, Kahn M, Downey GP 
In:
Am J Physiol Lung Cell Mol Physiol (2013) 304(6): 415-27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Geller R, Andino R, Frydman J 
In:
PLoS ONE (2013) 8(2): e56762 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yadav UC, Naura AS, Aguilera-Aguirre L, Boldogh I, Boulares HA, Calhoun WJ, Ramana KV, Srivastava SK 
In:
PLoS ONE (2013) 8(2): e57442 
Categories:
Primary Cells and Media 
Authors:
Bartis D, Csongei V, Weich A, Kiss E, Barko S, Kovacs T, Avdicevic M, D'Souza VK, Rapp J, Kvell K, Jakab L, Nyitrai M, Molnar TF, Thickett DR, Laszlo T, Pongracz JE. 
In:
PLoS ONE (2013) 8(3): e57393 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akram KM, Samad S, Spiteri MA, Forsyth NR. 
In:
Respir Res (2013) 14: 9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hsiao HM, Sapinoro RE, Thatcher TH, Croasdell A, Levy EP, Fulton RA, Olsen KC, Pollock SJ, Serhan CN, Phipps RP, Sime PJ. 
In:
PLoS ONE (2013) 8(3): e58258 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Geller R, Andino R, Frydman J. 
In:
PLoS ONE (2013) 8(2): e56762 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akram KM, Samad S, Spiteri MA, Forsyth NR 
In:
Respir Res (2013) 14: 9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ivarsson M, Schollin J, Björkqvist M 
In:
Acta Paediatr. (2012) 102(10): 1010-6 
Categories:
Primary Cells and Media, Bioassay  
Authors:
Valles I, Pajares MJ, Segura V, Guruceaga E, Gomez-Roman J, Blanco D, Tamura A, Montuenga LM, Pio R. 
In:
PLoS ONE (2012) Identificati: 7(8) 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lillehoj EP, Hyun SW, Feng C, Zhang L, Liu A, Guang W, Nguyen C, Luzina IG, Atamas SP, Passaniti A, Twaddell WS, Puché AC, Wang LX, Cross AS, Goldblum SE. 
In:
J Biol Chem (2012) 287(11): 8214-31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou J, Alvarez-Elizondo MB, Botvinick E, George SC 
In:
Journal of Applied Physiology (2012) 112(4): 627-37 
Categories:
Primary Cells and Media 
Authors:
Thomas KC, Roberts JK, Deering-Rice CE, Romero EG, Dull RO, Lee J, Yost GS, Reilly CA. 
In:
Am J Physiol Lung Cell Mol Physiol (2012) 302(1):: L111-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sundar IK, Chung S, Hwang JW, Lapek JD Jr, Bulger M, Friedman AE, Yao H, Davie JR, Rahman I 
In:
PLoS ONE (2012) 7: 2 
Categories:
Primary Cells and Media 
Authors:
Lizak M, Yarovinsky TO 
In:
Cell Host & Microbe (2012) 11(1): 70-80 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rao M1, Chinnasamy N, Hong JA, Zhang Y, Zhang M, Xi S, Liu F, Marquez VE, Morgan RA, Schrump DS. 
In:
Cancer Res (2011) 71(12): 4192-204 
Categories:
Primary Cells and Media 
Authors:
Fong V, Osterbur M, Capella C, Kim YE, Hine C, Gorbunova V, Seluanov A, Dewhurst S 
In:
PLoS ONE (2011) 6(12): e28714 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yadav UC, Aguilera-Aguirre L, Ramana KV, Boldogh I, Srivastava SK 
In:
PLoS ONE (2010) 5(12): e14440 
Categories:
Primary Cells and Media 
Authors:
Ezponda T, Pajares MJ, Agorreta J, Echeveste JI, López-Picazo JM, Torre W, Pio R, Montuenga LM. 
In:
Clin Cancer Res (2010) 16(16): 4113-25 
Categories:
Primary Cells and Media 
Authors:
Nguyen MH, Koinuma J, Ueda K, Ito T, Tsuchiya E, Nakamura Y, Daigo Y. 
In:
Cancer Res (2010) 70(13): 5337-47 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Harada Y, Kanehira M, Fujisawa Y, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y, Katagiri T. 
In:
Cancer Res (2010) 70(14): 5829-39 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu W, Tundwal K, Liang Q, Goplen N, Rozario S, Quayum N, Gorska M, Wenzel S, Balzar S, Alam R 
In:
Nat Cell Biol (2010) 30(7): 1783-99 
Categories:
Primary Cells and Media 
Authors:
Glazer CA, Smith IM, Ochs MF, Begum S, Westra W, Chang SS, Sun W, Bhan S, Khan Z, Ahrendt S, Califano JA. 
In:
PLoS ONE (2009) 4(12): e8189 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yadav UC, Naura AS, Aguilera-Aguirre L, Ramana KV, Boldogh I, Sur S, Boulares HA, Srivastava SK 
In:
J Immunol (2009) 183(7): 4723-32 
Categories:
Primary Cells and Media 
Authors:
Kadara H, Lacroix L, Behrens C, Solis L, Gu X, Lee JJ, Tahara E, Lotan D, Hong WK, Wistuba II, Lotan R. 
In:
Cancer Res (2009) 2(8): 702-11 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yadav UC, Ramana KV, Aguilera-Aguirre L, Boldogh I, Boulares HA, Srivastava SK 
In:
PLoS ONE (2009) 4(8): e6535 
Categories:
Primary Cells and Media 
Authors:
Martin FJ, Gomez MI, Wetzel DM, Memmi G, O\'Seaghdha M, Soong G, Schindler C, Prince A 
In:
J Clin Invest (2009) 119(7): 1931-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kolliputi N, Waxman AB. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 297(1): L6-16 
Categories:
Primary Cells and Media 
Authors:
Gras D, Chanez P, Urbach V, Vachier I, Godard P, Bonnans C 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 296(6): L970-8 
Categories:
Primary Cells and Media 
Authors:
Scharf S, Hippenstiel S, Flieger A, Suttorp N, N\'Guessan PD 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 298(5): L687-95 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moretto N1, Facchinetti F, Southworth T, Civelli M, Singh D, Patacchini R. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 296(5): L839-48 
Categories:
Primary Cells and Media 
Authors:
Luxen S, Noack D, Frausto M, Davanture S, Torbett BE, Knaus UG 
In:
J Cell Sci (2009) 122(Pt 8): 1238-47 
Categories:
Primary Cells and Media 
Authors:
Thaikoottathil JV, Martin RJ, Zdunek J, Weinberger A, Rino JG, Chu HW 
In:
Eur Respir J (2009) 33(4): 835-43 
Categories:
Primary Cells and Media 
Authors:
Hussain M, Rao M, Humphries AE, Hong JA, Liu F, Yang M, Caragacianu D, Schrump DS 
In:
Cancer Res (2009) 69(8): 3570-8 
Categories:
Primary Cells and Media 
Authors:
Lehmann M, Noack D, Wood M, Perego M, Knaus UG. 
In:
PLoS ONE (2009) 4(3): e4755 
Categories:
Primary Cells and Media 
Authors:
Chun J, Prince A. 
In:
Cell Host & Microbe (2009) 5(1): 47-58 
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