Jurkat

HumanT cell leukemia established from the peripheral blood of a 14 year old boy by Schneider et al.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE 96-CL-120 2e5 66-76% 76-84% Plasmid (general) 1 µg 20 µl Shuttle
V X-001 1e6 69-79% 64-84% Plasmid (general) 2 µg 100 µl I/II/2b
V X-001 1e6 87-89% 87-93% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 1e6 81-87% 64-86% Plasmid (general) 2 µg 100 µl I/II/2b
SE CL-120 2e5 75.9-77.9% 57-63% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CL-120 1e6 72-74% 69.7-73.7% Plasmid (general) 2 µg 100 µl 4D X-Unit
Automation AA1+AA2 96-CM-137 6e4 90-94% 70-72% Plasmid (general) 1 µg 20 µl Shuttle
SE CK-116 2e5 74% ±4 51% ±11 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CK-116 1e6 70% ±5 65% ±11 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (90)

Categories:
Transfection 
Authors:
Wang J, Li T, Zhou M, Hu Z, Zhou X, Zhou S, Wang N, Huang L, Zhao L, Cao Y, Xiao M, Ma D, Zhou P, Shang Z, Zhou J 
In:
Scientific Reports (2015) 5:18454: 10.1038 
Categories:
Transfection 
Authors:
Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M. 
In:
Nucleic Acids Res (2014) 42: 1-16 
Categories:
Transfection 
Authors:
Prabha Chandrasekaran, Victoria Moore, Monica Buckley, Joshua Spurrier, John H. Kehrl, Sundararajan Venkatesan 
In:
PLoS ONE (2014) 9(1): e86998 
Categories:
Transfection 
Authors:
Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E. 
In:
Genome Res (2013) 23(7): 1182-93 
Categories:
Primary Cells and Media 
Authors:
Miyamura K, Nishimura Y, Abo M, Wakita T, Shimizu H 
In:
J Gen Virol (2011) 92(Pt2): 287-91 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Pan W, Zhu S, Yuan M, Cui H, Wang L, Luo X, Li J, Zhou H, Tang Y, Shen N. 
In:
J Immunol (2010) 184(12): 6773-81 
Categories:
Transfection 
Authors:
Terme JM, Wencker M, Favre-Bonvin A, Bex F, Gazzolo L, Duc Dodon M, Jalinot P 
In:
J Virol (2008) 82(16): 7913-22 
Categories:
Transfection 
Authors:
Kirchhof MG, Chau LA, Lemke CD, Vardhana S, Darlington PJ, MÜ¡rquez ME, Taylor R, Rizkalla K, Blanca I, Dustin ML, Madrenas J 
In:
J Immunol (2008) 181(3): 1927-36 
Categories:
Transfection 
Authors:
Maucksch C, Aneja MK, Hennen E, Bohla A, Hoffmann F, Elfinger M, Rosenecker J, Rudolph C 
In:
Nucleic Acids Res (2008) epub: online 
Categories:
Transfection 
Authors:
Zhang SL, Kozak JA, Jiang W, Yeromin AV, Chen J, Yu Y, Penna A, Shen W, Chi V, Cahalan MD 
In:
J Biol Chem (2008) 283(25): 17662-71 
Categories:
Transfection 
Authors:
Fala F, Blalock WL, Tazzari P, Cappellini A, Chiarini F, Martinelli G, Tafuri A, McCubrey JA, Cocco L, Martelli AM 
In:
Mol Pharmacol (2008) epub: online 
Categories:
Transfection 
Authors:
Ku SC, Lee J, Lau J, Gurumurthy M, Ng R, Lwa SH, Lee J, Klase Z, Kashanchi F, Chao SH 
In:
J Virol (2008) 82(9): 4343-53 
Categories:
Transfection 
Authors:
Roesslein M, Schibilsky D, Muller L, Goebel U, Schwer C, Humar M, Schmidt R, Geiger KK, Pahl HL, Pannen BH, Loop T 
In:
J Pharmacol Exp Ther (2008) 325(1): 217-25 
Categories:
Transfection 
Authors:
Sowinski S, Jolly C, Berninghausen O, Purbhoo MA, Chauveau A, Köhler K, Oddos S, Eissmann P, Brodsky FM, Hopkins C, Onfelt B, Sattentau Q, Davis DM 
In:
Nat Cell Biol (2008) 10(2): 211-9 
Categories:
Transfection 
Authors:
Liu L, Schwartz B, Tsubota Y, Raines E, Kiyokawa H, Yonekawa K, Harlan JM, Schnapp LM 
In:
J Immunol (2008) 180(3): 1808-17 
Categories:
Transfection 
Authors:
Lioudyno MI, Kozak JA, Penna A, Safrina O, Zhang SL, Sen D, Roos J, Stauderman KA, Cahalan MD 
In:
Proc Natl Acad Sci USA (2008) 105(6): 2011-6 
Categories:
Transfection 
Authors:
Bras M, Yuste VJ, Roué G, Barbier S, Sancho P, Virely C, Rubio M, Baudet S, Esquerda JE, Merle-Béral H, Sarfati M, Susin SA 
In:
Mol Cell Biol (2007) 27(20): 7073-88 
Categories:
Transfection 
Authors:
Cheng J, Kydd AR, Nakase K, Noonan KM, Murakami A, Tao H, Dwyer M, Xu C, Zhu Q, Marasco WA 
In:
Blood (2007) 110(6): 2110-20 
Categories:
Transfection 
Authors:
Prasad A, Qamri Z, Wu J, Ganju RK 
In:
J Leukoc Biol (2007) 82(3): 465-76 
Categories:
Transfection 
Authors:
Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM 
In:
J Virol (2007) 81(17): 9346-56 
Categories:
Transfection 
Authors:
Razani-Boroujerdi S, Boyd RT, DÜ¡vila-GarcÜ­a MI, Nandi JS, Mishra NC, Singh SP, Pena-Philippides JC, Langley R, Sopori ML 
In:
J Immunol (2007) 179(5): 2889-98 
Categories:
Transfection 
Authors:
Takeuchi H, Buckler-White A, Goila-Gaur R, Miyagi E, Khan MA, Opi S, Kao S, Sokolskaja E, Pertel T, Luban J, Strebel K 
In:
J Virol (2007) 81(15): 8080-90 
Categories:
Transfection 
Authors:
Sekine Y, Tsuji S, Ikeda O, Sugiyma K, Oritani K, Shimoda K, Muromoto R, Ohbayashi N, Yoshimura A, Matsuda T 
In:
J Immunol (2007) 179(4): 2397-407 
Categories:
Transfection 
Authors:
Giri B, Dixit VD, Ghosh MC, Collins GD, Khan IU, Madara K, Weeraratna AT, Taub DD 
In:
Eur J Immunol (2007) 37(8): 2104-16 
Categories:
Transfection 
Authors:
Mitchell DA, Morton SU, Fernhoff NB, Marletta MA 
In:
Proc Natl Acad Sci USA (2007) 104(28): 11609-14 
Categories:
Transfection 
Authors:
Mor A, Campi G, Du G, Zheng Y, Foster DA, Dustin ML, Philips MR 
In:
Nat Cell Biol (2007) 9(6): 713-9 
Categories:
Transfection 
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA 
In:
Oncogene (2007) 26(26): 3797-810 
Categories:
Transfection 
Authors:
Bernhagen J, Krohn R, Lue H, Gregory JL, Zernecke A, Koenen RR, Dewor M, Georgiev I, Schober A, Leng L, Kooistra T, Fingerle-Rowson G, Ghezzi P, Kleemann R, McColl SR, Bucala R, Hickey MJ, Weber C 
In:
Nat Med (2007) 13(5): 587-596 
Categories:
Transfection 
Authors:
Kim HR, Hwang KA, Kim KC, Kang I 
In:
J Immunol (2007) 178(9): 5473-9 
Categories:
Transfection 
Authors:
Leal-Sanchez J, Couzinet A, Rossin A, Abdel-Sater F, Chakrabandhu K, Luci C, Anjuere F, Stebe E, Hancock D, Hueber AO 
In:
Cell Death Differ (2007) 14(4): 795-806 
Categories:
Transfection 
Authors:
Hirano S, Furutama D, Hanafusa T 
In:
Am J Physiol Regul Integr Comp Physiol (2007) 292(4): R1465-71 
Categories:
Transfection 
Authors:
Rueda D, Gaide O, Ho L, Lewkowicz E, Niedergang F, Hailfinger S, Rebeaud F, Guzzardi M, Conne B, Thelen M, Delon J, Ferch U, Mak TW, Ruland J, Schwaller J, Thome M 
In:
J Immunol (2007) 178(7): 4373-84 
Categories:
Transfection 
Authors:
Zimmerer JM, Lesinski GB, Kondadasula SV, Karpa VI, Lehman A, Raychaudhury A, Becknell B, Carson WE 3rd 
In:
J Immunol (2007) 178(8): 4832-45 
Categories:
Transfection 
Authors:
Gobessi S, Laurenti L, Longo PG, Sica S, Leone G, Efremov DG 
In:
Blood (2007) 109(5): 2032-9 
Categories:
Transfection 
Authors:
Ohnuma K, Uchiyama M, Yamochi T, Nishibashi K, Hosono O, Takahashi N, Kina S, Tanaka H, Lin X, Dang NH, Morimoto C 
In:
J Biol Chem (2007) 282(13): 10117-31 
Categories:
Transfection 
Authors:
Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, Reynes J, Corbeau P, Jeang KT, Benkirane M 
In:
Science (2007) 315(5818): 1579-82 
Categories:
Transfection 
Authors:
Mazurov D, Heidecker G, Derse D 
In:
J Biol Chem (2007) 282(6): 3896-903 
Categories:
Transfection 
Authors:
Huang Y, Yang H, Borg BB, Su X, Rhodes SL, Yang K, Tong X, Tang G, Howell CD, Rosen HR, Thio CL, Thomas DL, Alter HJ, Sapp RK, Liang TJ 
In:
Proc Natl Acad Sci USA (2007) 104(3): 985-90 
Categories:
Transfection 
Authors:
Crotti A, Neri F, Corti D, Ghezzi S, Heltai S, Baur A, Poli G, Santagostino E, Vicenzi E 
In:
J Virol (2006) 80(21): 10663-74 
Categories:
Transfection 
Authors:
Lopez-Anton N, Rudy A, Barth N, Schmitz ML, Pettit GR, Schulze-Osthoff K, Dirsch VM, Vollmar AM 
In:
J Biol Chem (2006) 281(44): 33078-86 
Categories:
Transfection 
Authors:
Tavano R, Contento RL, Baranda SJ, Soligo M, Tuosto L, Manes S, Viola A 
In:
Nat Cell Biol (2006) 8(11): 1270-6 
Categories:
Transfection 
Authors:
Vandenbussche CJ, Dakshanamurthy S, Posch PE, Hurley CK 
In:
J Immunol (2006) 177(8): 5347-57 
Categories:
Transfection 
Authors:
Thurau M, Everett H, Tapernoux M, Tschopp J, Thome M 
In:
Cell Death Differ (2006) 13(9): 1577-85 
Categories:
Transfection 
Authors:
Weigert A, Johann AM, von Knethen A, Schmidt H, Geisslinger G, Brune B 
In:
Blood (2006) 108(5): 1635-42 
Categories:
Transfection 
Authors:
Schieke SM, Phillips D, McCoy JP Jr, Aponte AM, Shen RF, Balaban RS, Finkel T 
In:
J Biol Chem (2006) 281(37): 27643-52 
Categories:
Transfection 
Authors:
Houtman JC, Yamaguchi H, Barda-Saad M, Braiman A, Bowden B, Appella E, Schuck P, Samelson LE 
In:
Nat Struct Mol Biol (2006) 13(9): 798-805 
Categories:
Transfection 
Authors:
Ozaki K, Sato H, Iida A, Mizuno H, Nakamura T, Miyamoto Y, Takahashi A, Tsunoda T, Ikegawa S, Kamatani N, Hori M, Nakamura Y, Tanaka T 
In:
Nat Genet (2006) 38(8): 921-5 
Categories:
Transfection 
Authors:
Jia W, Hegde VL, Singh NP, Sisco D, Grant S, Nagarkatti M, Nagarkatti PS 
In:
Mol Cancer Res (2006) 4(8): 549-62 
Categories:
Transfection 
Authors:
Mahmoudi T, Parra M, Vries RG, Kauder SE, Verrijzer CP, Ott M, Verdin E 
In:
J Biol Chem (2006) 281(29): 19960-8 
Categories:
Transfection 
Authors:
Fernandez-Vidal A, Ysebaert L, Didier C, Betous R, De Toni F, Prade-Houdellier N, Demur C, Contour-Galcera MO, Prevost GP, Ducommun B, Payrastre B, Racaud-Sultan C, Manenti S 
In:
Cancer Res (2006) 66(14): 7128-35 
Categories:
Transfection 
Authors:
Lietman SA, Kalinchinko N, Deng X, Kohanski R, Levine MA 
In:
Hum Mutat (2006) 27(7): 717-8 
Categories:
Transfection 
Authors:
Perlman M, Resh MD 
In:
Traffic (2006) 7(6): 731-45 
Categories:
Transfection 
Authors:
von Essen M, Nielsen MW, Bonefeld CM, Boding L, Larsen JM, Leitges M, Baier G, Odum N, Geisler C 
In:
J Immunol (2006) 176(12): 7502-10 
Categories:
Transfection 
Authors:
Krueger A, Fas SC, Giaisi M, Bleumink M, Merling A, Stumpf C, Baumann S, Holtkotte D, Bosch V, Krammer PH and Li-Weber M 
In:
Blood (2006) 107(10): 3933-9 
Categories:
Transfection 
Authors:
Anand AR, Ganju RK 
In:
J Biol Chem (2006) 281(18): 12289-99 
Categories:
Transfection 
Authors:
Imai K, Okamoto T 
In:
J Biol Chem (2006) 281(18): 12495-505 
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Categories:
Transfection 
Authors:
Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN and Figdor CG 
In:
Blood (2006) 107(8): 3212-20 
Categories:
Transfection 
Authors:
Huang C, Bi E, Hu Y, Deng W, Tian Z, Dong C, Hu Y, Sun B 
In:
J Immunol (2006) 176(7): 4173-81 
Categories:
Transfection 
Authors:
Lettau M, Qian J, Linkermann A, Latreille M, Larose L, Kabelitz D, Janssen O 
In:
Proc Natl Acad Sci USA (2006) 103(15): 5911-6 
Categories:
Transfection 
Authors:
Plant EP, Dinman JD 
In:
RNA (2006) 12(4): 666-73 
Categories:
Transfection 
Authors:
Xie L, Yamamoto B, Haoudi A, Semmes OJ and Green PL 
In:
Blood (2006) 107(5): 1980-8 
Categories:
Transfection 
Authors:
Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ 
In:
J Cell Biol (2006) 172(6): 923-35 
Categories:
Transfection 
Authors:
Weinberg MS, Villeneuve LM, Ehsani A, Amarzguioui M, Aagaard L, Chen ZX, Riggs AD, Rossi JJ and Morris KV 
In:
RNA (2006) 12(2): 256-262 
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Categories:
Transfection 
Authors:
Perez-Galan P, Roue G, Villamor N, Montserrat E, Campo E and Colomer D 
In:
Blood (2006) 107(1): 257-264 
Categories:
Transfection 
Authors:
Lieto LD, Maasho K, West D, Borrego F and Coligan JE 
In:
Genes Immun (2006) 7(1): 36-43 
Categories:
Transfection 
Authors:
Zipfel PA, Bunnell SC, Witherow DS, Gu JJ, Chislock EM, Ring C, Pendergast AM 
In:
Curr Biol (2006) 16(1): 35-46 
Categories:
Transfection 
Authors:
Guo Y, Chen C, Zheng Y, Zhang J, Tao X, Liu S, Zheng D and Liu Y 
In:
J Biol Chem (2005) 280(51): 41940-41952 
Categories:
Transfection 
Authors:
Skov S, Pedersen MT, Andresen L, Thor Straten P, Woetmann A and Odum N 
In:
Cancer Res (2005) 65(23): 11136-11145 
Categories:
Transfection 
Authors:
Sommer K, Guo B, Pomerantz JL, Bandaranayake AD, Moreno-Garcia ME, Ovechkina YL, Rawlings DJ 
In:
Immunity (2005) 23(6): 561-74 
Categories:
Transfection 
Authors:
Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck TJ, Ellisman MH, Stauderman KA and Cahalan MD 
In:
Nature (2005) 437(7060): 902-905 
Categories:
Transfection 
Authors:
Dreikhausen U, Hiebenthal-Millow K, Bartels M, Resch K and Nourbakhsh M 
In:
Mol Cell Biol (2005) 25(17): 7473-7483 
Categories:
Transfection 
Authors:
Nejmeddine M, Barnard AL, Tanaka Y, Taylor GP and Bangham CR 
In:
J Biol Chem (2005) 280(33): 29653-29660 
Categories:
Transfection 
Authors:
Hiraragi H, Michael B, Nair A, Silic-Benussi M, Ciminale V and Lairmore M 
In:
J Virol (2005) 79(15): 9449-9457 
Categories:
Transfection 
Authors:
Yang Y, Yang F, Xiong Z, Yan Y, Wang X, Nishino M, Mirkovic D, Nguyen J, Wang H, Yang XF 
In:
Oncogene (2005) 24(30): 4778-4788 
Categories:
Transfection 
Authors:
Kiyokawa E, Baba T, Otsuka N, Makino A, Ohno S and Kobayashi T 
In:
J Biol Chem (2005) 280(25): 24072-24084 
Categories:
Transfection 
Authors:
Kawaida R, Yamada R, Kobayashi K, Tokuhiro S, Suzuki A, Kochi Y, Chang X, Sekine A, Tsunoda T, Sawada T, Furukawa H, Nakamura Y and Yamamoto K 
In:
Genes Immun (2005) 6(3): 194-202 
Categories:
Transfection 
Authors:
Ingold K, Zumsteg A, Tardivel A, Huard B, Steiner QG, Cachero TG, Qiang F, Gorelik L, Kalled SL, Acha-Orbea H, Rennert PD, Tschopp J, Schneider P 
In:
J Exp Med (2005) 201(9): 1375-1383 
Categories:
Transfection 
Authors:
Wirtz S, Becker C, Fantini MC, Nieuwenhuis EE, Tubbe I, Galle PR, Schild HJ, Birkenbach M, Blumberg RS and Neurath MF 
In:
J Immunol (2005) 174(5): 2814-2824 
Categories:
Transfection 
Authors:
Gaikwad A, Poblenz A, Haridas V, Zhang C, Duvic M and Gutterman J 
In:
Clin Cancer Res (2005) 11(5): 1953-1962 
Categories:
Transfection 
Authors:
Song EJ, Yim SH, Kim E, Kim NS and Lee KJ 
In:
Mol Cell Biol (2005) 25(6): 2511-2524 
Categories:
Transfection 
Authors:
Puissant B, Roubinet F, Dellacasagrande J, Massip P, Abbal M, Pasquier C, Izopet J, Blancher A 
In:
AIDS (2005) 19(6): 627-30 
Categories:
Transfection 
Authors:
Zhang Y, Erdmann F, Baumgrass R, Schutkowski M and Fischer G 
In:
J Biol Chem (2005) 280(6): 4842-4850 
Categories:
Transfection 
Authors:
Dombroski D, Houghtling RA, Labno CM, Precht P, Takesono A, Caplen NJ, Billadeau DD, Wange RL, Burkhardt JK and Schwartzberg PL 
In:
J Immunol (2005) 174(3): 1385-1392 
Categories:
Transfection 
Authors:
Lacalle RA, Gomez-Mouton C, Barber DF, Jimenez-Baranda S, Mira E, Martinez-A C, Carrera AC, Manes S 
In:
J Cell Sci (2004) 117(Pt 25): 6207-6215 
Categories:
Transfection 
Authors:
Frazer-Abel AA, Baksh S, Fosmire SP, Willis D, Pierce AM, Meylemans H, Linthicum DS, Burakoff SJ, Coons T, Bellgrau D and Modiano JF 
In:
J Pharmacol Exp Ther (2004) 311(2): 758-769 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109 
Categories:
Transfection 
Authors:
Bunnell SC, Hong DI, Kardon JR, Yamazaki T, McGlade CJ, Barr VA and Samelson LE 
In:
J Cell Biol (2002) 158(7): 1263-1275 
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