CH12F3

subclone of the lgM+ CH12.LX lymphoma B cell line

Cell Type:
B cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L D-023 2e6 99% 84% Plasmid (general) 2 µg 100 µl I/II/2b
L X-001 2e6 85% 80% Plasmid (general) 2 µg 100 µl I/II/2b
L X-005 2e6 97% 80% Plasmid (general) 2 µg 100 µl I/II/2b
SF CA-137 1e6 93.2% 69.6% Plasmid (general) 0.5 µg 20 µl 4D X-Unit