J558L
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B lymphocyte, BALB/c myeloma
Cell Type:
B cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
A-023
3e6
40%
85%
Plasmid (general)
5 µg
100 µl
I/II/2b
T
T-016
3e6
63%
37%
Plasmid (general)
5 µg
100 µl
I/II/2b
T
T-001
3e6
35%
88%
Plasmid (general)
5 µg
100 µl
I/II/2b
T
G-016
3e6
50%
70%
Plasmid (general)
5 µg
100 µl
I/II/2b
Citations
The initiation of antigen-induced B cell antigen receptor signaling viewed in living cells by fluorescence resonance energy transfer
Categories:
Transfection
Authors:
Tolar P, Sohn HW and Pierce SK
In:
Nat Immunol (2005) 6(11): 1168-1176