U-937

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human histiocytic lymphoma, promonocytic, T cell like

Cell Type:
Monocyte
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF

EP-100

 2e5 32-39% 85% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
C W-001 1e6 66.9% ±2 84.9% ±1 Plasmid (general) 2 µg 100 µl I/II/2b
SF 96-EP-100 2e5 36% ±3 85% ±4 Plasmid (general) 0.2 µg 20 µl 96-well

Citations (45)

Activating c-KIT mutations confer oncogenic cooperativity and rescue RUNX1/ETO-induced DNA damage and apoptosis in human primary CD34+ hematopoietic progenitors.
Categories:
Primary Cells and Media 
Authors:
Wichmann C, Quagliano-Lo Coco I, Yildiz Ö, Chen-Wichmann L, Weber H, Syzonenko T, Döring C, Brendel C, Ponnusamy K, Kinner A, Brandts C, Henschler R, Grez M 
In:
Leukemia (2015) 29(2): 279-89 
A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee JH, Sohn JH, Ryu SY, Hong CS, Moon KD, Park JW 
In:
J Cell Mol Med (2013) 17(10): 1271-81 
Rap1 activation is required for Fc gamma receptor-dependent phagocytosis
Categories:
Transfection 
Authors:
Chung J, Serezani CH, Huang SK, Stern JN, Keskin DB, Jagirdar R, Brock TG, Aronoff DM, Peters-Golden M 
In:
J Immunol (2008) 181(8): 5501-9 
Constitutive activation of PI3-kinase signaling pathway downregulates TLR4-mediated TNF-alpha release in alveolar macrophages from asymptomatic HIV+ persons in vitro
Categories:
Transfection 
Authors:
Tachado SD, Li X, Swan K, Patel N, Koziel H 
In:
J Biol Chem (2008) 283(48): 33191-8 
Regulation of arsenic trioxide-induced cellular responses by Mnk1 and Mnk2
Categories:
Transfection 
Authors:
Dolniak B, Katsoulidis E, Carayol N, Altman JK, Redig AJ, Tallman MS, Ueda T, Watanabe-Fukunaga R, Fukunaga R, Platanias LC 
In:
J Biol Chem (2008) 283(18): 12034-42 
Filamin A Stabilizes Fc-gamma-RI Surface Expression and Prevents Its Lysosomal Routing
Categories:
Transfection 
Authors:
Beekman JM, van der Poel CE, van der Linden JA, van den Berg DL, van den Berghe PV, van de Winkel JG, Leusen JH 
In:
J Immunol (2008) 180(6): 3938-45 
Chemokine stromal cell-derived factor-1 induction by C/EBP activation is associated with all-trans-retinoic acid-induced leukemic cell differentiation
Categories:
Transfection 
Authors:
Kim K, Kim HH, Kim JH, Choi YH, Kim YH, Cheong J 
In:
J Leukoc Biol (2007) 82(5): 1332-9 
Target proteins of C/EBPp30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9
Categories:
Transfection 
Authors:
Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre G 
In:
Blood (2007) 110(9): 3301-9 
Pathogenesis of aryl hydrocarbon receptor-mediated development of lymphoma is associated with increased cyclooxygenase-2 expression
Categories:
Transfection 
Authors:
Vogel CF, Li W, Sciullo E, Newman J, Hammock B, Reader JR, Tuscano J, Matsumura F 
In:
Am J Pathol (2007) 171(5): 1538-48 
Novel RUNX1 isoforms determine the fate of acute myeloid leukemia cells by controlling CD56 expression
Categories:
Transfection 
Authors:
Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A 
In:
Blood (2007) 110(6): 2027-33 
A post-transcriptional pathway represses monocyte VEGF-A expression and angiogenic activity
Categories:
Transfection 
Authors:
Ray PS, Fox PL 
In:
EMBO J (2007) 26(14): 3360-72 
Does HLA-B27 influence the monocyte inflammatory response to lipopolysaccharide?
Categories:
Transfection 
Authors:
Goodall JC, Ellis L, Yeo GS, Gaston JS 
In:
Rheumatology (2007) 46(2): 232-7 
Protein Kinase CK2 as an Unfavorable Prognostic Marker and Novel Therapeutic Target in Acute Myeloid Leukemia
Categories:
Transfection 
Authors:
Kim JS, Eom JI, Cheong JW, Choi AJ, Lee JK, Yang WI, Min YH 
In:
Clin Cancer Res (2007) 13(3): 1019-28 
Nitric oxide down-regulates polo-like kinase 1 through a proximal promoter cell cycle gene homology region
Categories:
Transfection 
Authors:
Zhang J, Wang S, Kern S, Cui X, Danner RL 
In:
J Biol Chem (2007) 282(2): 1003-9 
Mcl-1 down-regulation potentiates ABT-737 lethality by cooperatively inducing Bak activation and Bax translocation
Categories:
Transfection 
Authors:
Chen S, Dai Y, Harada H, Dent P, Grant S 
In:
Cancer Res (2007) 67(2): 782-91 
L13a blocks 48S assembly: role of a general initiation factor in mRNA-specific translational control
Categories:
Transfection 
Authors:
Kapasi P, Chaudhuri S, Vyas K, Baus D, Komar AA, Fox PL, Merrick WC, Mazumder B 
In:
Mol Cell (2007) 25(1): 113-26 
Proteomic discovery of Max as a novel interacting partner of C/EBPalpha: a Myc/Max/Mad link
Categories:
Transfection 
Authors:
Zada AA, Pulikkan JA, Bararia D, Geletu M, Trivedi AK, Balkhi MY, Hiddemann WD, Tenen DG, Behre HM, Behre G 
In:
Leukemia (2006) 20(12): 2137-2146 
Histone deacetylase inhibitors deplete enhancer of zeste 2 and associated polycomb repressive complex 2 proteins in human acute leukemia cells
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K 
In:
Mol Cancer Ther (2006) 5(12): 3096-104 
Tribbles homolog 2 inactivates C/EBPalpha and causes acute myelogenous leukemia
Categories:
Transfection 
Authors:
Keeshan K, He Y, Wouters BJ, Shestova O, Xu L, Sai H, Rodriguez CG, Maillard I, Tobias JW, Valk P, Carroll M, Aster JC, Delwel R, Pear WS 
In:
Cancer Cell (2006) 10(5): 401-11 
The Three-Substituted Indolinone Cyclin-Dependent Kinase 2 Inhibitor 3-[1-(3H-Imidazol-4-yl)-meth-(Z)-ylidene]-5-methoxy-1,3-dihydro-indol-2-one (SU9516) Kills Human Leukemia Cells via Down-Regulation of Mcl-1 through a Transcriptional Mechanism
Categories:
Transfection 
Authors:
Gao N, Kramer L, Rahmani M, Dent P, Grant S 
In:
Mol Pharmacol (2006) 70(2): 645-55 
Cell Adhesion Regulates CDC25A Expression and Proliferation in Acute Myeloid Leukemia
Categories:
Transfection 
Authors:
Fernandez-Vidal A, Ysebaert L, Didier C, Betous R, De Toni F, Prade-Houdellier N, Demur C, Contour-Galcera MO, Prevost GP, Ducommun B, Payrastre B, Racaud-Sultan C, Manenti S 
In:
Cancer Res (2006) 66(14): 7128-35 
A crosstalk between the Wnt and the adhesion-dependent signaling pathways governs the chemosensitivity of acute myeloid leukemia
Categories:
Transfection 
Authors:
De Toni F, Racaud-Sultan C, Chicanne G, Mas VM, Cariven C, Mesange F, Salles JP, Demur C, Allouche M, Payrastre B, Manenti S, Ysebaert L 
In:
Oncogene (2006) 25(22): 3113-3122 
Cytoplasmic function of mutant PML and PML-RARalpha
Categories:
Transfection 
Authors:
Bellodi C, Kindle K, Bernassola F, Dinsdale D, Cossarizza A, Melino G, Heery D, Salomoni P 
In:
J Biol Chem (2006) 281(20): 14465-14473 
Regulation of apoptosis/necrosis execution in cadmium-treated human promonocytic cells under different forms of oxidative stress
Categories:
Transfection 
Authors:
Sancho P, Fernandez C, Yuste VJ, Amran D, Ramos AM, de Blas E, Susin SA, Aller P 
In:
Apoptosis (2006) 11(5): 673-86 
The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction
Categories:
Transfection 
Authors:
Yoshimi R, Yamaji S, Suzuki A, Mishima W, Okamura M, Obana T, Matsuda C, Miwa Y, Ohno S, Ishigatsubo Y 
In:
J Immunol (2006) 176(6): 3611-24 
Involvement of Group VIA Calcium-Independent Phospholipase A2 in Macrophage Engulfment of Hydrogen Peroxide-Treated U937 Cells
Categories:
Transfection 
Authors:
Perez R, Balboa MA and Balsinde J 
In:
J Immunol (2006) 176(4): 2555-2561 
The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Synergistic antileukemic interactions between 2-medroxyestradiol (2-ME) and histone deacetylase inhibitors involve Akt down-regulation and oxidative stress
Categories:
Transfection 
Authors:
Gao N, Rahmani M, Shi X, Dent P and Grant S 
In:
Blood (2006) 107(1): 241-249 
Recognition of double stranded RNA by human toll like receptor 3 and downstream receptor signaling requires multimerisation and an acidic pH
Categories:
Transfection 
Authors:
de Bouteiller O, Merck E, Hasan UA, Hubac S, Benguigui B, Trinchieri G, Bates EE and Caux C 
In:
J Biol Chem (2005) 280(46): 38133-38145 
Cleavage of p21/WAF1/CIP1 by proteinase 3 modulates differentiation of a monocytic cell line. Molecular analysis of the cleavage site
Categories:
Transfection 
Authors:
Dublet B, Ruello A, Pederzoli M, Hajjar E, Courbebaisse M, Canteloup S, Reuter N and Witko-Sarsat V 
In:
J Biol Chem (2005) 80(34): 30242-30253 
CBFB-SMMHC is correlated with increased calreticulin expression and suppresses the granulocytic differentiation factor CEBPA in AML with inv(16)
Categories:
Transfection 
Authors:
Helbling D, Mueller BU, Timchenko NA, Schardt J, Eyer M, Betts DR, Jotterand M, Meyer-Monard S, Fey MF and Pabst T 
In:
Blood (2005) 106(4): 1369-1375 
HIV-1 Nef down-regulates the hemochromatosis protein HFE, manipulating cellular iron homeostasis
Categories:
Transfection 
Authors:
Drakesmith H, Chen N, Ledermann H, Screaton G, Townsend A and Xu XN 
In:
Proc Natl Acad Sci USA (2005) 102(31): 11017-11022 
Blockade of Histone Deacetylase Inhibitor-Induced RelA/p65 Acetylation and NF-B Activation Potentiates Apoptosis in Leukemia Cells through a Process Mediated by Oxidative Damage, XIAP Downregulation, and c-Jun N-Terminal Kinase 1 Activation
Categories:
Transfection 
Authors:
Dai Y, Rahmani M, Dent P and Grant S 
In:
Mol Cell Biol (2005) 25(13): 5429-5444 
2-Methoxyestradiol-induced apoptosis in human leukemia cells proceeds through a reactive oxygen species and Akt-dependent process
Categories:
Transfection 
Authors:
Gao N, Rahmani M, Dent and Grant S 
In:
Oncogene (2005) 24(23): 3797-3809 
Rapamycin and UCN-01 synergistically induce apoptosis in human leukemia cells through a process that is regulated by the Raf-1/MEK/ERK, Akt, and JNK signal transduction pathways
Categories:
Transfection 
Authors:
Hahn M, Li W, Yu C, Rahmani M, Dent P and Grant S 
In:
Mol Cancer Ther (2005) 4(3): 457-470 
Coadministration of histone deacetylase inhibitors and perifosine synergistically induces apoptosis in human leukemia cells through Akt and ERK1/2 inactivation and the generation of ceramide and reactive oxygen species
Categories:
Transfection 
Authors:
Rahmani M, Reese E, Dai Y, Bauer C, Payne SG, Dent P, Spiegel S and Grant S 
In:
Cancer Res (2005) 65(6): 2422-2432 
Farnesyltransferase inhibitors interact synergistically with the CHK1 inhibitor UCN-01 to induce apoptosis in human leukemia cells through interruption of both AKT and MEK/ERK pathways and activation of SEK1/JNK
Categories:
Transfection 
Authors:
Dai Y, Rahmani M, Pei XY, Khanna P, Han SI, Mitchell C, Dent P, Grant S 
In:
Blood (2005) 105(4): 1706-1716 
Dynamic changes in Mcl-1 expression regulate macrophage viability or commitment to apoptosis during bacterial clearance
Categories:
Transfection 
Authors:
Marriott HM, Bingle CD, Read RC, Braley KE, Kroemer G, Hellewell PG, Craig RW, Whyte MK and Dockrell DH 
In:
J Clin Invest (2005) 115(2): 359-368 
Atypical PKC-zeta regulates SDF-1-mediated migration and development of human CD34(+) progenitor cells
Categories:
Transfection 
Authors:
Petit I, Goichberg P, Spiegel A, Peled A, Brodie C, Seger R, Nagler A, Alon R and Lapidot T 
In:
J Clin Invest (2005) 115(1): 168-176 
Protein kinase C mediates retinoic acid and phorbol myristate acetate-induced phospholipid scramblase 1 gene expression: its role in leukemic cell differentiation
Categories:
Transfection 
Authors:
Zhao KW, Li X, Zhao Q, Huang Y, Li D, Peng ZG, Shen WZ, Zhao J, Zhou Q, Chen Z, Sims PJ, Wiedmer T and Chen GQ 
In:
Blood (2004) 104(12): 3731-3738 
Interleukin (IL)-15 and IL-2 Reciprocally Regulate Expression of the Chemokine Receptor CX3CR1 through Selective NFAT1- and NFAT2-dependent Mechanisms
Categories:
Transfection 
Authors:
Barlic J, McDermott DH, Merrell MN, Gonzales J, Via LE and Murphy PM 
In:
J Biol Chem (2004) 279(47): 48520-48534 
Cytoplasmic mislocalization of p27Kip1 protein is associated with constitutive phosphorylation of Akt or protein kinase B and poor prognosis in acute myelogenous leukemia
Categories:
Transfection 
Authors:
Min YH, Cheong JW, Kim JY, Eom JI, Lee ST, Hahn JS, Ko YW and Lee MH 
In:
Cancer Res (2004) 64(15): 5225-5231 
Phosphorylation of C/EBPalpha inhibits granulopoiesis
Categories:
Transfection 
Authors:
Ross SE, Radomska HS, Wu B, Zhang P, Winnay JN, Bajnok L, Wright WS, Schaufele F, Tenen DG and MacDougald OA 
In:
Mol Cell Biol (2004) 24(2): 675-686 
Nucleofection as an efficient nonviral transfection method for human monocytic cells
Categories:
Transfection 
Authors:
Martinet W, Schrijvers DM and Kockx MM 
In:
Biotechnol Lett (2003) 25: 1025-1029 
Oxidation-triggered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways for apoptosis in human leukaemic cells stimulated by epigallocatechin-3-gallate (EGCG): a distinct pathway from those of chemically induced and receptor-mediated apoptosis
Categories:
Transfection 
Authors:
Saeki K, Kobayashi N, Inazawa Y, Zhang H, Nishitoh H, Ichijo H, Saeki K, Isemura M, Yuo A 
In:
Biochem J (2002) 368(Pt 3): 705-20 
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