Endothelial, aortic, cow (bAEC)

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Primary endothelial cells from bovine aorta

Cell Type:
Aortic Endo.
Tissue Origin:
aortic
Species:
bovine
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Endothelial Cell Growth Medium - Microvascular

This medium has been discontinued end of 2023. As alternative we recommend the EGM-2 MV CC-3202

Developed for microvascular and coronary artery endothelial cells
Same basal medium as in EGM
Final serum concentration is 5%
EGM-MV can be used to grow most Clonetics Endothelial Cells

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Endothelial, basic M-003 5e5 70-90% siRNA 35 ng 100 µl I/II/2b

Citations

Rap1 and its effector KRIT1/CCM1 regulate beta-catenin signaling.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Glading AJ, Ginsberg MH. 
In:
Dis Model Mech (2010) 3(1-2): 73-83 
KRIT-1/CCM1 is a Rap1 effector that regulates endothelial cell cell junctions
Categories:
Transfection 
Authors:
Glading A, Han J, Stockton RA, Ginsberg MH 
In:
J Cell Biol (2007) 179(2): 247-54 
Induction of Vascular Permeability: betaPIX and GIT1 Scaffold the Activation of Extracellular Signal-regulated Kinase by PAK
Categories:
Transfection 
Authors:
Stockton R, Reutershan J, Scott D, Sanders J, Ley K, Schwartz MA 
In:
Mol Biol Cell (2007) 18(6): 2346-55 
Hypertonicity triggers RhoA-dependent assembly of myosin-containing striated polygonal actin networks in endothelial cells
Categories:
Transfection 
Authors:
Malek AM, Xu C, Kim ES, Alper SL 
In:
Am J Physiol Cell Physiol (2007) 292(5): C1645-59 

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